MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears
Abstract Hibernating brown bears, Ursus arctos , undergo extended periods of inactivity and yet these large hibernators are resilient to muscle disuse atrophy. Physiological characteristics associated with atrophy resistance in bear muscle have been examined (e.g., muscle mechanics, neural activity)...
Published in: | Journal of Cellular Physiology |
---|---|
Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2019
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/jcp.29294 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcp.29294 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jcp.29294 |
id |
crwiley:10.1002/jcp.29294 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/jcp.29294 2024-06-02T08:15:37+00:00 MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears Luu, Bryan E. Lefai, Etienne Giroud, Sylvain Swenson, Jon E. Chazarin, Blandine Gauquelin‐Koch, Guillemette Arnemo, Jon M. Evans, Alina L. Bertile, Fabrice Storey, Kenneth B. Natural Sciences and Engineering Research Council of Canada Université de Strasbourg Miljødirektoratet Naturvårdsverket Svenska Jägareförbundet Austrian Science Fund Centre National d’Etudes Spatiales 2019 http://dx.doi.org/10.1002/jcp.29294 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcp.29294 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jcp.29294 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Cellular Physiology volume 235, issue 4, page 3984-3993 ISSN 0021-9541 1097-4652 journal-article 2019 crwiley https://doi.org/10.1002/jcp.29294 2024-05-03T11:53:45Z Abstract Hibernating brown bears, Ursus arctos , undergo extended periods of inactivity and yet these large hibernators are resilient to muscle disuse atrophy. Physiological characteristics associated with atrophy resistance in bear muscle have been examined (e.g., muscle mechanics, neural activity) but roles for molecular signaling/regulatory mechanisms in the resistance to muscle wasting in bears still require investigation. Using quantitative reverse transcription PCR (RT‐qPCR), the present study characterized the responses of 36 microRNAs linked with development, metabolism, and regeneration of skeletal muscle, in the vastus lateralis of brown bears comparing winter hibernating and summer active animals. Relative levels of mRNA of selected genes ( mef2a, pax7, id2, prkaa1 , and mstn ) implicated upstream and downstream of the microRNAs were examined. Results indicated that hibernation elicited a myogenic microRNA, or “myomiR”, response via MEF2A‐mediated signaling. Upregulation of MEF2A‐controlled miR‐1 and miR‐206 and respective downregulation of pax7 and id2 mRNA are suggestive of responses that promote skeletal muscle maintenance. Increased levels of metabolic microRNAs, such as miR‐27, miR‐29, and miR‐33, may facilitate metabolic suppression during hibernation via mechanisms that decrease glucose uptake and fatty acid oxidation. This study identified myomiR‐mediated mechanisms for the promotion of muscle regeneration, suppression of ubiquitin ligases, and resistance to muscle atrophy during hibernation mediated by observed increases in miR‐206, miR‐221, miR‐31, miR‐23a, and miR‐29b. This was further supported by the downregulation of myomiRs associated with a muscle injury and inflammation (miR‐199a and miR‐223) during hibernation. The present study provides evidence of myomiR‐mediated signaling pathways that are activated during hibernation to maintain skeletal muscle functionality in brown bears. Article in Journal/Newspaper Ursus arctos Wiley Online Library Journal of Cellular Physiology 235 4 3984 3993 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Hibernating brown bears, Ursus arctos , undergo extended periods of inactivity and yet these large hibernators are resilient to muscle disuse atrophy. Physiological characteristics associated with atrophy resistance in bear muscle have been examined (e.g., muscle mechanics, neural activity) but roles for molecular signaling/regulatory mechanisms in the resistance to muscle wasting in bears still require investigation. Using quantitative reverse transcription PCR (RT‐qPCR), the present study characterized the responses of 36 microRNAs linked with development, metabolism, and regeneration of skeletal muscle, in the vastus lateralis of brown bears comparing winter hibernating and summer active animals. Relative levels of mRNA of selected genes ( mef2a, pax7, id2, prkaa1 , and mstn ) implicated upstream and downstream of the microRNAs were examined. Results indicated that hibernation elicited a myogenic microRNA, or “myomiR”, response via MEF2A‐mediated signaling. Upregulation of MEF2A‐controlled miR‐1 and miR‐206 and respective downregulation of pax7 and id2 mRNA are suggestive of responses that promote skeletal muscle maintenance. Increased levels of metabolic microRNAs, such as miR‐27, miR‐29, and miR‐33, may facilitate metabolic suppression during hibernation via mechanisms that decrease glucose uptake and fatty acid oxidation. This study identified myomiR‐mediated mechanisms for the promotion of muscle regeneration, suppression of ubiquitin ligases, and resistance to muscle atrophy during hibernation mediated by observed increases in miR‐206, miR‐221, miR‐31, miR‐23a, and miR‐29b. This was further supported by the downregulation of myomiRs associated with a muscle injury and inflammation (miR‐199a and miR‐223) during hibernation. The present study provides evidence of myomiR‐mediated signaling pathways that are activated during hibernation to maintain skeletal muscle functionality in brown bears. |
author2 |
Natural Sciences and Engineering Research Council of Canada Université de Strasbourg Miljødirektoratet Naturvårdsverket Svenska Jägareförbundet Austrian Science Fund Centre National d’Etudes Spatiales |
format |
Article in Journal/Newspaper |
author |
Luu, Bryan E. Lefai, Etienne Giroud, Sylvain Swenson, Jon E. Chazarin, Blandine Gauquelin‐Koch, Guillemette Arnemo, Jon M. Evans, Alina L. Bertile, Fabrice Storey, Kenneth B. |
spellingShingle |
Luu, Bryan E. Lefai, Etienne Giroud, Sylvain Swenson, Jon E. Chazarin, Blandine Gauquelin‐Koch, Guillemette Arnemo, Jon M. Evans, Alina L. Bertile, Fabrice Storey, Kenneth B. MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
author_facet |
Luu, Bryan E. Lefai, Etienne Giroud, Sylvain Swenson, Jon E. Chazarin, Blandine Gauquelin‐Koch, Guillemette Arnemo, Jon M. Evans, Alina L. Bertile, Fabrice Storey, Kenneth B. |
author_sort |
Luu, Bryan E. |
title |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_short |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_full |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_fullStr |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_full_unstemmed |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_sort |
micrornas facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1002/jcp.29294 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcp.29294 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jcp.29294 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Journal of Cellular Physiology volume 235, issue 4, page 3984-3993 ISSN 0021-9541 1097-4652 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jcp.29294 |
container_title |
Journal of Cellular Physiology |
container_volume |
235 |
container_issue |
4 |
container_start_page |
3984 |
op_container_end_page |
3993 |
_version_ |
1800739862401253376 |