Mechanisms of petroleum hydrocarbon toxicity: Destruction of liver microsomal and mitochondrial calcium pump activities by a prudhoe bay crude oil
Abstract Administration of Prudhoe Bay crude oil (PBCO) to rats resulted in an abrupt drop in liver mitochondrial and microsomal ATP‐dependent calcium uptake activity. Also, in vitro incubations of either mitochondria or microsomes in the presence of a dimethyl sulfoxide (DMSO) extract of PBCO resul...
| Published in: | Journal of Biochemical Toxicology |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
1986
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1002/jbt.2570010404 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbt.2570010404 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbt.2570010404 |
| Summary: | Abstract Administration of Prudhoe Bay crude oil (PBCO) to rats resulted in an abrupt drop in liver mitochondrial and microsomal ATP‐dependent calcium uptake activity. Also, in vitro incubations of either mitochondria or microsomes in the presence of a dimethyl sulfoxide (DMSO) extract of PBCO resulted in a dose‐dependent inhibition of calcium influx. The release of calcium from calciumloaded mitochondria and microsomes was also observed in the presence of the PBCO extract. At concentrations which effect calcium sequestration, the PBCO extract produced swelling of mitochondria. Microsomal ATPase activity in the presence or absence of calcium was unaffected by PBCO. The results indicate that increased permeability of the membranes to calcium is a contributory factor in the inhibition of calcium uptake by PBCO. |
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