Effects of synthetic peptido‐leukotrienes on bone resorption in vitro
Abstract Peptido‐leukotrienes are short‐lived organic molecules known to have potent biological effects as mediators of inflammation, hypersensitivity and respiratory disorders. However, little is known concerning their effects on bone cells. We have shown previously that stromal cells isolated from...
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crwiley:10.1002/jbmr.5650110413 2023-12-03T10:30:48+01:00 Effects of synthetic peptido‐leukotrienes on bone resorption in vitro Garcia, C. Qiao, M. Chen, D. Kirchen, M. Gallwitz, W. Mundy, G.R. Bonewald, L.F. 1996 http://dx.doi.org/10.1002/jbmr.5650110413 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbmr.5650110413 https://onlinelibrary.wiley.com/doi/full/10.1002/jbmr.5650110413 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Bone and Mineral Research volume 11, issue 4, page 521-529 ISSN 0884-0431 1523-4681 Orthopedics and Sports Medicine Endocrinology, Diabetes and Metabolism journal-article 1996 crwiley https://doi.org/10.1002/jbmr.5650110413 2023-11-09T13:58:10Z Abstract Peptido‐leukotrienes are short‐lived organic molecules known to have potent biological effects as mediators of inflammation, hypersensitivity and respiratory disorders. However, little is known concerning their effects on bone cells. We have shown previously that stromal cells isolated from a human giant cell tumor secrete 5‐HETE (5‐hydroxyeicosatetraenoic acid) and the peptido‐leukotrienes, also known as the cysteinyl leukotrienes LTC 4 , LTD 4 , and LTE 4 . These eicosanoids were shown to stimulate the multinucleated giant cells obtained from these tumors to form resorption lacunae on sperm whale dentine. Here, we show that the peptido‐leukotrienes also stimulate isolated avian osteoclast‐like cells to form resorption lacunae and to increase their content of tartrateresistant acid phosphatase. LTD 4 increased 45 Ca release from murine calvarial bone organ cultures, but not from fetal rat long bone cultures. Isolated avian osteoclast‐like cells were chosen to perform receptor binding studies, as this population is the most homogenous source of osteoclasts available. After the precursors had fused to form multinucleated cells, receptor binding assays were performed. Scatchard analysis of saturation binding data showed a single class of binding sites, with a dissociation constant (K d ) of 0.53 nM and a receptor density of 5,200 receptors per cell. Competition binding studies showed receptor specificity using a specific LTD 4 receptor antagonist ZM 198,615. These data show that the peptido‐leukotrienes activate highly enriched populations of isolated avian osteoclast‐like cells, and also that specific LTD 4 receptors are present in this cell population. Article in Journal/Newspaper Sperm whale Wiley Online Library (via Crossref) Journal of Bone and Mineral Research 11 4 521 529 |
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Open Polar |
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Wiley Online Library (via Crossref) |
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crwiley |
language |
English |
topic |
Orthopedics and Sports Medicine Endocrinology, Diabetes and Metabolism |
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Orthopedics and Sports Medicine Endocrinology, Diabetes and Metabolism Garcia, C. Qiao, M. Chen, D. Kirchen, M. Gallwitz, W. Mundy, G.R. Bonewald, L.F. Effects of synthetic peptido‐leukotrienes on bone resorption in vitro |
topic_facet |
Orthopedics and Sports Medicine Endocrinology, Diabetes and Metabolism |
description |
Abstract Peptido‐leukotrienes are short‐lived organic molecules known to have potent biological effects as mediators of inflammation, hypersensitivity and respiratory disorders. However, little is known concerning their effects on bone cells. We have shown previously that stromal cells isolated from a human giant cell tumor secrete 5‐HETE (5‐hydroxyeicosatetraenoic acid) and the peptido‐leukotrienes, also known as the cysteinyl leukotrienes LTC 4 , LTD 4 , and LTE 4 . These eicosanoids were shown to stimulate the multinucleated giant cells obtained from these tumors to form resorption lacunae on sperm whale dentine. Here, we show that the peptido‐leukotrienes also stimulate isolated avian osteoclast‐like cells to form resorption lacunae and to increase their content of tartrateresistant acid phosphatase. LTD 4 increased 45 Ca release from murine calvarial bone organ cultures, but not from fetal rat long bone cultures. Isolated avian osteoclast‐like cells were chosen to perform receptor binding studies, as this population is the most homogenous source of osteoclasts available. After the precursors had fused to form multinucleated cells, receptor binding assays were performed. Scatchard analysis of saturation binding data showed a single class of binding sites, with a dissociation constant (K d ) of 0.53 nM and a receptor density of 5,200 receptors per cell. Competition binding studies showed receptor specificity using a specific LTD 4 receptor antagonist ZM 198,615. These data show that the peptido‐leukotrienes activate highly enriched populations of isolated avian osteoclast‐like cells, and also that specific LTD 4 receptors are present in this cell population. |
format |
Article in Journal/Newspaper |
author |
Garcia, C. Qiao, M. Chen, D. Kirchen, M. Gallwitz, W. Mundy, G.R. Bonewald, L.F. |
author_facet |
Garcia, C. Qiao, M. Chen, D. Kirchen, M. Gallwitz, W. Mundy, G.R. Bonewald, L.F. |
author_sort |
Garcia, C. |
title |
Effects of synthetic peptido‐leukotrienes on bone resorption in vitro |
title_short |
Effects of synthetic peptido‐leukotrienes on bone resorption in vitro |
title_full |
Effects of synthetic peptido‐leukotrienes on bone resorption in vitro |
title_fullStr |
Effects of synthetic peptido‐leukotrienes on bone resorption in vitro |
title_full_unstemmed |
Effects of synthetic peptido‐leukotrienes on bone resorption in vitro |
title_sort |
effects of synthetic peptido‐leukotrienes on bone resorption in vitro |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1002/jbmr.5650110413 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbmr.5650110413 https://onlinelibrary.wiley.com/doi/full/10.1002/jbmr.5650110413 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Journal of Bone and Mineral Research volume 11, issue 4, page 521-529 ISSN 0884-0431 1523-4681 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jbmr.5650110413 |
container_title |
Journal of Bone and Mineral Research |
container_volume |
11 |
container_issue |
4 |
container_start_page |
521 |
op_container_end_page |
529 |
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1784256840730673152 |