Ligand‐ and proton‐linked conformational changes of the ferrous 2/2 hemoglobin of Pseudoalteromonas haloplanktis TAC125
Abstract The spectroscopic and ligand‐binding properties of a 2/2 globin from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 have been studied in the ferrous state. It displays two major conformations characterized by CO‐association rates that differ by a factor of 20, with relative f...
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crwiley:10.1002/iub.492 2024-09-15T17:46:22+00:00 Ligand‐ and proton‐linked conformational changes of the ferrous 2/2 hemoglobin of Pseudoalteromonas haloplanktis TAC125 Giordano, Daniela Russo, Roberta Ciaccio, Chiara Howes, Barry D. di Prisco, Guido Marden, Michael C. Hui Bon Hoa, Gaston Smulevich, Giulietta Coletta, Massimo Verde, Cinzia 2011 http://dx.doi.org/10.1002/iub.492 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fiub.492 https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1002/iub.492 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor IUBMB Life volume 63, issue 7, page 566-573 ISSN 1521-6543 1521-6551 journal-article 2011 crwiley https://doi.org/10.1002/iub.492 2024-07-11T04:36:49Z Abstract The spectroscopic and ligand‐binding properties of a 2/2 globin from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 have been studied in the ferrous state. It displays two major conformations characterized by CO‐association rates that differ by a factor of 20, with relative fractions that depend on pH. A dynamic equilibrium is found between the two conformations, as indicated by an enhanced slower phase when lower CO levels were used to allow a longer time to facilitate the transition. The deoxy form, in the absence of external ligands, is a mixture of a predominant six‐coordinate low spin form and a five‐coordinate high‐spin state; the proportion of low spin increasing at alkaline pH. In addition, at temperatures above the physiological temperature of 1 °C, an enhanced tendency of the protein to oxidize is observed. © 2011 IUBMB IUBMB Life, 63(7): 566–573, 2011 Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library IUBMB Life 63 7 566 573 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The spectroscopic and ligand‐binding properties of a 2/2 globin from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 have been studied in the ferrous state. It displays two major conformations characterized by CO‐association rates that differ by a factor of 20, with relative fractions that depend on pH. A dynamic equilibrium is found between the two conformations, as indicated by an enhanced slower phase when lower CO levels were used to allow a longer time to facilitate the transition. The deoxy form, in the absence of external ligands, is a mixture of a predominant six‐coordinate low spin form and a five‐coordinate high‐spin state; the proportion of low spin increasing at alkaline pH. In addition, at temperatures above the physiological temperature of 1 °C, an enhanced tendency of the protein to oxidize is observed. © 2011 IUBMB IUBMB Life, 63(7): 566–573, 2011 |
format |
Article in Journal/Newspaper |
author |
Giordano, Daniela Russo, Roberta Ciaccio, Chiara Howes, Barry D. di Prisco, Guido Marden, Michael C. Hui Bon Hoa, Gaston Smulevich, Giulietta Coletta, Massimo Verde, Cinzia |
spellingShingle |
Giordano, Daniela Russo, Roberta Ciaccio, Chiara Howes, Barry D. di Prisco, Guido Marden, Michael C. Hui Bon Hoa, Gaston Smulevich, Giulietta Coletta, Massimo Verde, Cinzia Ligand‐ and proton‐linked conformational changes of the ferrous 2/2 hemoglobin of Pseudoalteromonas haloplanktis TAC125 |
author_facet |
Giordano, Daniela Russo, Roberta Ciaccio, Chiara Howes, Barry D. di Prisco, Guido Marden, Michael C. Hui Bon Hoa, Gaston Smulevich, Giulietta Coletta, Massimo Verde, Cinzia |
author_sort |
Giordano, Daniela |
title |
Ligand‐ and proton‐linked conformational changes of the ferrous 2/2 hemoglobin of Pseudoalteromonas haloplanktis TAC125 |
title_short |
Ligand‐ and proton‐linked conformational changes of the ferrous 2/2 hemoglobin of Pseudoalteromonas haloplanktis TAC125 |
title_full |
Ligand‐ and proton‐linked conformational changes of the ferrous 2/2 hemoglobin of Pseudoalteromonas haloplanktis TAC125 |
title_fullStr |
Ligand‐ and proton‐linked conformational changes of the ferrous 2/2 hemoglobin of Pseudoalteromonas haloplanktis TAC125 |
title_full_unstemmed |
Ligand‐ and proton‐linked conformational changes of the ferrous 2/2 hemoglobin of Pseudoalteromonas haloplanktis TAC125 |
title_sort |
ligand‐ and proton‐linked conformational changes of the ferrous 2/2 hemoglobin of pseudoalteromonas haloplanktis tac125 |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1002/iub.492 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fiub.492 https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1002/iub.492 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
IUBMB Life volume 63, issue 7, page 566-573 ISSN 1521-6543 1521-6551 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/iub.492 |
container_title |
IUBMB Life |
container_volume |
63 |
container_issue |
7 |
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566 |
op_container_end_page |
573 |
_version_ |
1810494444528467968 |