Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms
Abstract The Antarctic is a potentially important library of microbial resources and new bioactive substances. In this study, microorganisms were isolated from surface water samples collected from different sites of the Antarctic. 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide ( MT...
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crwiley:10.1002/fsn3.273 2024-06-23T07:46:05+00:00 Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms Zheng, Lanhong Yang, Kangli Liu, Jia Sun, Mi Zhu, Jiancheng Lv, Mei Kang, Daole Wang, Wei Xing, Mengxin Li, Zhao Chinese Academy of Fishery Sciences 2015 http://dx.doi.org/10.1002/fsn3.273 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Ffsn3.273 https://onlinelibrary.wiley.com/doi/pdf/10.1002/fsn3.273 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/fsn3.273 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Food Science & Nutrition volume 4, issue 2, page 198-206 ISSN 2048-7177 2048-7177 journal-article 2015 crwiley https://doi.org/10.1002/fsn3.273 2024-06-13T04:23:54Z Abstract The Antarctic is a potentially important library of microbial resources and new bioactive substances. In this study, microorganisms were isolated from surface water samples collected from different sites of the Antarctic. 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide ( MTT ) assay‐based cytotoxicity‐tracking method was used to identify Antarctic marine microorganism resources for antitumor lead compounds. The results showed that a total of 129 Antarctic microorganism strains were isolated. Twelve strains showed potent cytotoxic activities, among which a Gram‐negative, rod‐shaped bacterium, designated as N11‐8 was further studied. Phylogenetic analysis based on 16S rRNA gene sequence showed that N11‐8 belongs to the genus Bacillus . Fermented active products of N11‐8 with molecular weights of 1–30 kDa had higher inhibitory effects on different cancaer cells, such as BEL ‐7402 human hepatocellular carcinoma cells, U251 human glioma cells, RKO human colon carcinoma cells, A549 human lung carcinoma cells, and MCF ‐7 human breast carcinoma cells. However, they displayed lower cytotoxicity against HFL 1 human normal fibroblast lung cells. However, they displayed lower cytotoxicity against HFL 1 human normal fibroblast lung cells. Microscopic observations showed that the fermented active products have inhibitory activity on BEL ‐7402 cells similar to that of mitomycin C. Further studies indicated that the fermented active products have high pH and high thermal stability. In conclusion, most strains isolated in this study may be developed as promising sources for the discovery of antitumor bioactive substances. The fermented active products of Antarctic marine Bacillus sp. N11‐ 8 are expected to be applied in the prevention and treatment of cancer. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The Antarctic Food Science & Nutrition 4 2 198 206 |
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Wiley Online Library |
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English |
description |
Abstract The Antarctic is a potentially important library of microbial resources and new bioactive substances. In this study, microorganisms were isolated from surface water samples collected from different sites of the Antarctic. 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide ( MTT ) assay‐based cytotoxicity‐tracking method was used to identify Antarctic marine microorganism resources for antitumor lead compounds. The results showed that a total of 129 Antarctic microorganism strains were isolated. Twelve strains showed potent cytotoxic activities, among which a Gram‐negative, rod‐shaped bacterium, designated as N11‐8 was further studied. Phylogenetic analysis based on 16S rRNA gene sequence showed that N11‐8 belongs to the genus Bacillus . Fermented active products of N11‐8 with molecular weights of 1–30 kDa had higher inhibitory effects on different cancaer cells, such as BEL ‐7402 human hepatocellular carcinoma cells, U251 human glioma cells, RKO human colon carcinoma cells, A549 human lung carcinoma cells, and MCF ‐7 human breast carcinoma cells. However, they displayed lower cytotoxicity against HFL 1 human normal fibroblast lung cells. However, they displayed lower cytotoxicity against HFL 1 human normal fibroblast lung cells. Microscopic observations showed that the fermented active products have inhibitory activity on BEL ‐7402 cells similar to that of mitomycin C. Further studies indicated that the fermented active products have high pH and high thermal stability. In conclusion, most strains isolated in this study may be developed as promising sources for the discovery of antitumor bioactive substances. The fermented active products of Antarctic marine Bacillus sp. N11‐ 8 are expected to be applied in the prevention and treatment of cancer. |
author2 |
Chinese Academy of Fishery Sciences |
format |
Article in Journal/Newspaper |
author |
Zheng, Lanhong Yang, Kangli Liu, Jia Sun, Mi Zhu, Jiancheng Lv, Mei Kang, Daole Wang, Wei Xing, Mengxin Li, Zhao |
spellingShingle |
Zheng, Lanhong Yang, Kangli Liu, Jia Sun, Mi Zhu, Jiancheng Lv, Mei Kang, Daole Wang, Wei Xing, Mengxin Li, Zhao Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms |
author_facet |
Zheng, Lanhong Yang, Kangli Liu, Jia Sun, Mi Zhu, Jiancheng Lv, Mei Kang, Daole Wang, Wei Xing, Mengxin Li, Zhao |
author_sort |
Zheng, Lanhong |
title |
Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms |
title_short |
Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms |
title_full |
Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms |
title_fullStr |
Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms |
title_full_unstemmed |
Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms |
title_sort |
screening of microorganisms from antarctic surface water and cytotoxicity metabolites from antarctic microorganisms |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1002/fsn3.273 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Ffsn3.273 https://onlinelibrary.wiley.com/doi/pdf/10.1002/fsn3.273 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/fsn3.273 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Food Science & Nutrition volume 4, issue 2, page 198-206 ISSN 2048-7177 2048-7177 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/fsn3.273 |
container_title |
Food Science & Nutrition |
container_volume |
4 |
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2 |
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198 |
op_container_end_page |
206 |
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1802643861364801536 |