Immunochemical quantification of metallothioneins in marine mollusks: Characterization of a metal exposure bioindicator
Abstract A recombinant metallothionein CgMT1, from the Pacific oyster Crassostrea gigas , was synthesized and used as antigen in the development of antibodies and a specific enzyme‐linked immunosorbent assay (ELISA). The ELISA showed that rabbit anti‐CgMT1 IgG reacted with purified CgMT1 and MTs fro...
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crwiley:10.1002/etc.5620210517 2024-06-23T07:52:17+00:00 Immunochemical quantification of metallothioneins in marine mollusks: Characterization of a metal exposure bioindicator Boutet, Isabelle Tanguy, Arnaud Auffret, Michel Riso, Ricardo Moraga, Dario 2002 http://dx.doi.org/10.1002/etc.5620210517 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.5620210517 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5620210517 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Toxicology and Chemistry volume 21, issue 5, page 1009-1014 ISSN 0730-7268 1552-8618 journal-article 2002 crwiley https://doi.org/10.1002/etc.5620210517 2024-06-04T06:46:48Z Abstract A recombinant metallothionein CgMT1, from the Pacific oyster Crassostrea gigas , was synthesized and used as antigen in the development of antibodies and a specific enzyme‐linked immunosorbent assay (ELISA). The ELISA showed that rabbit anti‐CgMT1 IgG reacted with purified CgMT1 and MTs from other marine bivalves, indicating its suitability as a reagent to quantify MTs and for monitoring of metal contamination in field animals. Application of this assay to tissues excised from metal exposed C. gigas showed that MT induction reached a saturation level in gills that was not observed in digestive gland. Quantification of MTs in gills and digestive gland of field‐collected C. gigas showed that the MT level depended on the metal concentrations at the collection sites and may have been influenced by salinity variations at estuarine sites. Oysters from metal‐contaminated estuarine sites showed lower MT concentrations than those from nonestuarine contaminated sites. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific Environmental Toxicology and Chemistry 21 5 1009 1014 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract A recombinant metallothionein CgMT1, from the Pacific oyster Crassostrea gigas , was synthesized and used as antigen in the development of antibodies and a specific enzyme‐linked immunosorbent assay (ELISA). The ELISA showed that rabbit anti‐CgMT1 IgG reacted with purified CgMT1 and MTs from other marine bivalves, indicating its suitability as a reagent to quantify MTs and for monitoring of metal contamination in field animals. Application of this assay to tissues excised from metal exposed C. gigas showed that MT induction reached a saturation level in gills that was not observed in digestive gland. Quantification of MTs in gills and digestive gland of field‐collected C. gigas showed that the MT level depended on the metal concentrations at the collection sites and may have been influenced by salinity variations at estuarine sites. Oysters from metal‐contaminated estuarine sites showed lower MT concentrations than those from nonestuarine contaminated sites. |
format |
Article in Journal/Newspaper |
author |
Boutet, Isabelle Tanguy, Arnaud Auffret, Michel Riso, Ricardo Moraga, Dario |
spellingShingle |
Boutet, Isabelle Tanguy, Arnaud Auffret, Michel Riso, Ricardo Moraga, Dario Immunochemical quantification of metallothioneins in marine mollusks: Characterization of a metal exposure bioindicator |
author_facet |
Boutet, Isabelle Tanguy, Arnaud Auffret, Michel Riso, Ricardo Moraga, Dario |
author_sort |
Boutet, Isabelle |
title |
Immunochemical quantification of metallothioneins in marine mollusks: Characterization of a metal exposure bioindicator |
title_short |
Immunochemical quantification of metallothioneins in marine mollusks: Characterization of a metal exposure bioindicator |
title_full |
Immunochemical quantification of metallothioneins in marine mollusks: Characterization of a metal exposure bioindicator |
title_fullStr |
Immunochemical quantification of metallothioneins in marine mollusks: Characterization of a metal exposure bioindicator |
title_full_unstemmed |
Immunochemical quantification of metallothioneins in marine mollusks: Characterization of a metal exposure bioindicator |
title_sort |
immunochemical quantification of metallothioneins in marine mollusks: characterization of a metal exposure bioindicator |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1002/etc.5620210517 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.5620210517 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5620210517 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Environmental Toxicology and Chemistry volume 21, issue 5, page 1009-1014 ISSN 0730-7268 1552-8618 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/etc.5620210517 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
21 |
container_issue |
5 |
container_start_page |
1009 |
op_container_end_page |
1014 |
_version_ |
1802643541452652544 |