The effect of stress on toxicant‐dependent cytochrome P450 enzyme responses in the arctic charr ( Salvelinus alpinus)
Abstract In the present study we investigated the effect of stress and cortisol on cytochrome P450 (CYP) expression in Arctic charr exposed to benzo[ a ]pyrene (B a P). Expression of hepatic CYP1A and CYP3A was monitored 8 d after a single oral dose of B a P (10 mg/kg fish) and compared to that in u...
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Online Access: | http://dx.doi.org/10.1002/etc.5620201118 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.5620201118 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5620201118 |
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crwiley:10.1002/etc.5620201118 2024-06-02T08:00:04+00:00 The effect of stress on toxicant‐dependent cytochrome P450 enzyme responses in the arctic charr ( Salvelinus alpinus) Jørgensen, Even Hjalmar Celander, Malin Goksøyr, Anders Iwata, Munehico 2001 http://dx.doi.org/10.1002/etc.5620201118 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.5620201118 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5620201118 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Toxicology and Chemistry volume 20, issue 11, page 2523-2529 ISSN 0730-7268 1552-8618 journal-article 2001 crwiley https://doi.org/10.1002/etc.5620201118 2024-05-03T11:48:57Z Abstract In the present study we investigated the effect of stress and cortisol on cytochrome P450 (CYP) expression in Arctic charr exposed to benzo[ a ]pyrene (B a P). Expression of hepatic CYP1A and CYP3A was monitored 8 d after a single oral dose of B a P (10 mg/kg fish) and compared to that in unexposed fish. During this period the fish were subjected to one of the following stress regimes: no stress, no stress and cortisol implantation, 10 min of daily handling and confinement stress, and confinement stress during the last 6 h before sampling. In B a P‐exposed fish daily stress resulted in significantly lower (53%) CYP1A protein levels as compared to those in unstressed fish. For CYP1A catalytic activity (measured as 7‐ethoxyresorufin‐ O ‐deethylase [EROD] activity), the suppressive response to stress was less pronounced. These results contrast to previous findings of a potentiation by corticosteroids on xenobiotic‐dependent CYP1A induction in vitro in cultured fish hepatic cells. No effects of high cortisol levels or B a P were found on the steroid‐metabolizing CYP3A enzyme levels. The lack of any alterations in the CYP3A protein level indicates that CYP3A expression is not inducible by cortisol in the Arctic charr under the conditions used here. The conclusion was made that short‐term stress associated with sampling (i.e., 6 h of confinement stress before sampling) of wild charr does not compromise the EROD activity as a reliable biomarker. Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus Wiley Online Library Arctic Environmental Toxicology and Chemistry 20 11 2523 2529 |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract In the present study we investigated the effect of stress and cortisol on cytochrome P450 (CYP) expression in Arctic charr exposed to benzo[ a ]pyrene (B a P). Expression of hepatic CYP1A and CYP3A was monitored 8 d after a single oral dose of B a P (10 mg/kg fish) and compared to that in unexposed fish. During this period the fish were subjected to one of the following stress regimes: no stress, no stress and cortisol implantation, 10 min of daily handling and confinement stress, and confinement stress during the last 6 h before sampling. In B a P‐exposed fish daily stress resulted in significantly lower (53%) CYP1A protein levels as compared to those in unstressed fish. For CYP1A catalytic activity (measured as 7‐ethoxyresorufin‐ O ‐deethylase [EROD] activity), the suppressive response to stress was less pronounced. These results contrast to previous findings of a potentiation by corticosteroids on xenobiotic‐dependent CYP1A induction in vitro in cultured fish hepatic cells. No effects of high cortisol levels or B a P were found on the steroid‐metabolizing CYP3A enzyme levels. The lack of any alterations in the CYP3A protein level indicates that CYP3A expression is not inducible by cortisol in the Arctic charr under the conditions used here. The conclusion was made that short‐term stress associated with sampling (i.e., 6 h of confinement stress before sampling) of wild charr does not compromise the EROD activity as a reliable biomarker. |
format |
Article in Journal/Newspaper |
author |
Jørgensen, Even Hjalmar Celander, Malin Goksøyr, Anders Iwata, Munehico |
spellingShingle |
Jørgensen, Even Hjalmar Celander, Malin Goksøyr, Anders Iwata, Munehico The effect of stress on toxicant‐dependent cytochrome P450 enzyme responses in the arctic charr ( Salvelinus alpinus) |
author_facet |
Jørgensen, Even Hjalmar Celander, Malin Goksøyr, Anders Iwata, Munehico |
author_sort |
Jørgensen, Even Hjalmar |
title |
The effect of stress on toxicant‐dependent cytochrome P450 enzyme responses in the arctic charr ( Salvelinus alpinus) |
title_short |
The effect of stress on toxicant‐dependent cytochrome P450 enzyme responses in the arctic charr ( Salvelinus alpinus) |
title_full |
The effect of stress on toxicant‐dependent cytochrome P450 enzyme responses in the arctic charr ( Salvelinus alpinus) |
title_fullStr |
The effect of stress on toxicant‐dependent cytochrome P450 enzyme responses in the arctic charr ( Salvelinus alpinus) |
title_full_unstemmed |
The effect of stress on toxicant‐dependent cytochrome P450 enzyme responses in the arctic charr ( Salvelinus alpinus) |
title_sort |
effect of stress on toxicant‐dependent cytochrome p450 enzyme responses in the arctic charr ( salvelinus alpinus) |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1002/etc.5620201118 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.5620201118 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5620201118 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
Environmental Toxicology and Chemistry volume 20, issue 11, page 2523-2529 ISSN 0730-7268 1552-8618 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/etc.5620201118 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
20 |
container_issue |
11 |
container_start_page |
2523 |
op_container_end_page |
2529 |
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1800744085762342912 |