Effects of piperonyl butoxide and β‐naphthoflavone on cytochrome P4501A expression and activity in Atlantic salmon ( Salmo salar L.)
Abstract The effects of piperonyl butoxide (PBO) and β‐naphthoflavone (BNF) on cytochrome P4501A (CYP1A) expression and activity in juvenile Atlantic salmon ( Salmo salar L.) with regard to time and temperature was investigated. The time exposure study was performed at 8 °C, and the results show tha...
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crwiley:10.1002/etc.5620160304 2024-06-02T08:03:37+00:00 Effects of piperonyl butoxide and β‐naphthoflavone on cytochrome P4501A expression and activity in Atlantic salmon ( Salmo salar L.) Grøsvik, Bjørn Einar Larsen, Håvard E. Goksøyr, Anders 1997 http://dx.doi.org/10.1002/etc.5620160304 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.5620160304 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5620160304 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Toxicology and Chemistry volume 16, issue 3, page 415-423 ISSN 0730-7268 1552-8618 journal-article 1997 crwiley https://doi.org/10.1002/etc.5620160304 2024-05-03T12:00:38Z Abstract The effects of piperonyl butoxide (PBO) and β‐naphthoflavone (BNF) on cytochrome P4501A (CYP1A) expression and activity in juvenile Atlantic salmon ( Salmo salar L.) with regard to time and temperature was investigated. The time exposure study was performed at 8 °C, and the results show that PBO, although acutely functioning as a CYP1A inhibitor, is able to induce CYP1A expression in salmon liver. Both PBO and BNF give highest induction of CYP1A mRNA 48 h after intraperitoneal injection (five‐ and 14‐fold, respectively). The mRNA levels induced by PBO and BNF were sustained during the 8 d of the experiment (four‐ and 11‐fold, respectively). The CYP1A protein content measured by enzyme‐linked immunosorbent assay demonstrated the highest induction by PBO 8 d after exposure (eightfold) and by BNF 4 d after exposure (11‐fold). Activity of CYP1A measured by ethoxyresorufin‐ O ‐deethylase (EROD) demonstrated inhibition after PBO treatment the first 24 h after exposure, followed by threefold induction from 48 h and to the end of the experiment (8 d). β‐Naphthoflavone strongly induced EROD activity, with the highest levels occurring 4 d after treatment (56‐fold) and 8 d after treatment (22‐fold). In the temperature study, the results demonstrated temperature compensation, as salmon acclimated to 7°C for 3 weeks had a significantly higher EROD activity than those acclimated to 11 and 15°C. This was not reflected in significantly higher levels of reduced nicotinamide adenine dinucleotide phosphate cytochrome c reductase activity or CYP1A protein. The inductive properties of PBO and BNF on CYP1A expression was also demonstrated in primary cultures of salmon hepatocytes. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Environmental Toxicology and Chemistry 16 3 415 423 |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract The effects of piperonyl butoxide (PBO) and β‐naphthoflavone (BNF) on cytochrome P4501A (CYP1A) expression and activity in juvenile Atlantic salmon ( Salmo salar L.) with regard to time and temperature was investigated. The time exposure study was performed at 8 °C, and the results show that PBO, although acutely functioning as a CYP1A inhibitor, is able to induce CYP1A expression in salmon liver. Both PBO and BNF give highest induction of CYP1A mRNA 48 h after intraperitoneal injection (five‐ and 14‐fold, respectively). The mRNA levels induced by PBO and BNF were sustained during the 8 d of the experiment (four‐ and 11‐fold, respectively). The CYP1A protein content measured by enzyme‐linked immunosorbent assay demonstrated the highest induction by PBO 8 d after exposure (eightfold) and by BNF 4 d after exposure (11‐fold). Activity of CYP1A measured by ethoxyresorufin‐ O ‐deethylase (EROD) demonstrated inhibition after PBO treatment the first 24 h after exposure, followed by threefold induction from 48 h and to the end of the experiment (8 d). β‐Naphthoflavone strongly induced EROD activity, with the highest levels occurring 4 d after treatment (56‐fold) and 8 d after treatment (22‐fold). In the temperature study, the results demonstrated temperature compensation, as salmon acclimated to 7°C for 3 weeks had a significantly higher EROD activity than those acclimated to 11 and 15°C. This was not reflected in significantly higher levels of reduced nicotinamide adenine dinucleotide phosphate cytochrome c reductase activity or CYP1A protein. The inductive properties of PBO and BNF on CYP1A expression was also demonstrated in primary cultures of salmon hepatocytes. |
format |
Article in Journal/Newspaper |
author |
Grøsvik, Bjørn Einar Larsen, Håvard E. Goksøyr, Anders |
spellingShingle |
Grøsvik, Bjørn Einar Larsen, Håvard E. Goksøyr, Anders Effects of piperonyl butoxide and β‐naphthoflavone on cytochrome P4501A expression and activity in Atlantic salmon ( Salmo salar L.) |
author_facet |
Grøsvik, Bjørn Einar Larsen, Håvard E. Goksøyr, Anders |
author_sort |
Grøsvik, Bjørn Einar |
title |
Effects of piperonyl butoxide and β‐naphthoflavone on cytochrome P4501A expression and activity in Atlantic salmon ( Salmo salar L.) |
title_short |
Effects of piperonyl butoxide and β‐naphthoflavone on cytochrome P4501A expression and activity in Atlantic salmon ( Salmo salar L.) |
title_full |
Effects of piperonyl butoxide and β‐naphthoflavone on cytochrome P4501A expression and activity in Atlantic salmon ( Salmo salar L.) |
title_fullStr |
Effects of piperonyl butoxide and β‐naphthoflavone on cytochrome P4501A expression and activity in Atlantic salmon ( Salmo salar L.) |
title_full_unstemmed |
Effects of piperonyl butoxide and β‐naphthoflavone on cytochrome P4501A expression and activity in Atlantic salmon ( Salmo salar L.) |
title_sort |
effects of piperonyl butoxide and β‐naphthoflavone on cytochrome p4501a expression and activity in atlantic salmon ( salmo salar l.) |
publisher |
Wiley |
publishDate |
1997 |
url |
http://dx.doi.org/10.1002/etc.5620160304 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.5620160304 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5620160304 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Environmental Toxicology and Chemistry volume 16, issue 3, page 415-423 ISSN 0730-7268 1552-8618 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/etc.5620160304 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
16 |
container_issue |
3 |
container_start_page |
415 |
op_container_end_page |
423 |
_version_ |
1800748192860471296 |