Effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals
Abstract The effects of in vitro exposure of beluga whale splenocytes and thymocytes to different concentrations of mercury chloride (HgCl 2 ), cadmium chloride (CdCl 2 ), and lead chloride (PbCl 2 ) were evaluated. The cells were cultured for 66 h with either concanavalin A (Con‐A), phytohemaggluti...
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crwiley:10.1002/etc.5620150815 2024-04-21T07:58:26+00:00 Effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals Bernier, Jacques Fournier, Michel De Guise, Sylvain Martineau, Daniel Beland, Pierre 1996 http://dx.doi.org/10.1002/etc.5620150815 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.5620150815 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5620150815 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Toxicology and Chemistry volume 15, issue 8, page 1357-1364 ISSN 0730-7268 1552-8618 Health, Toxicology and Mutagenesis Environmental Chemistry journal-article 1996 crwiley https://doi.org/10.1002/etc.5620150815 2024-03-28T08:26:55Z Abstract The effects of in vitro exposure of beluga whale splenocytes and thymocytes to different concentrations of mercury chloride (HgCl 2 ), cadmium chloride (CdCl 2 ), and lead chloride (PbCl 2 ) were evaluated. The cells were cultured for 66 h with either concanavalin A (Con‐A), phytohemagglutinin‐P, or without mitogen, after which percentage of cell death and proliferation were evaluated. Increased percentage of cell death was observed in Con‐A‐stimulated thymocytes cultured with HgCl 2 , while the viability of splenocytes was not affected by exposure to metals. Decreased splenocyte and thymocyte proliferation was observed with the highest concentration of HgCl 2 and CdCl 2 (10 −5 M), while lower concentrations of these metals (10 −6 and 10 −7 M) as well as all the different concentrations of PbCl 2 (10 −4 , 10 −5 , and 10 −6 M) did not significantly influence cell proliferation. Concentrations of metals that were found to affect the proliferation of beluga lymphocytes are similar to those found in the liver of beluga whales from wild populations. Article in Journal/Newspaper Beluga Beluga whale Beluga* Wiley Online Library Environmental Toxicology and Chemistry 15 8 1357 1364 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Health, Toxicology and Mutagenesis Environmental Chemistry |
spellingShingle |
Health, Toxicology and Mutagenesis Environmental Chemistry Bernier, Jacques Fournier, Michel De Guise, Sylvain Martineau, Daniel Beland, Pierre Effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals |
topic_facet |
Health, Toxicology and Mutagenesis Environmental Chemistry |
description |
Abstract The effects of in vitro exposure of beluga whale splenocytes and thymocytes to different concentrations of mercury chloride (HgCl 2 ), cadmium chloride (CdCl 2 ), and lead chloride (PbCl 2 ) were evaluated. The cells were cultured for 66 h with either concanavalin A (Con‐A), phytohemagglutinin‐P, or without mitogen, after which percentage of cell death and proliferation were evaluated. Increased percentage of cell death was observed in Con‐A‐stimulated thymocytes cultured with HgCl 2 , while the viability of splenocytes was not affected by exposure to metals. Decreased splenocyte and thymocyte proliferation was observed with the highest concentration of HgCl 2 and CdCl 2 (10 −5 M), while lower concentrations of these metals (10 −6 and 10 −7 M) as well as all the different concentrations of PbCl 2 (10 −4 , 10 −5 , and 10 −6 M) did not significantly influence cell proliferation. Concentrations of metals that were found to affect the proliferation of beluga lymphocytes are similar to those found in the liver of beluga whales from wild populations. |
format |
Article in Journal/Newspaper |
author |
Bernier, Jacques Fournier, Michel De Guise, Sylvain Martineau, Daniel Beland, Pierre |
author_facet |
Bernier, Jacques Fournier, Michel De Guise, Sylvain Martineau, Daniel Beland, Pierre |
author_sort |
Bernier, Jacques |
title |
Effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals |
title_short |
Effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals |
title_full |
Effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals |
title_fullStr |
Effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals |
title_full_unstemmed |
Effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals |
title_sort |
effects of in vitro exposure of beluga whale splenocytes and thymocytes to heavy metals |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1002/etc.5620150815 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.5620150815 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.5620150815 |
genre |
Beluga Beluga whale Beluga* |
genre_facet |
Beluga Beluga whale Beluga* |
op_source |
Environmental Toxicology and Chemistry volume 15, issue 8, page 1357-1364 ISSN 0730-7268 1552-8618 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/etc.5620150815 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
15 |
container_issue |
8 |
container_start_page |
1357 |
op_container_end_page |
1364 |
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1796939584030900224 |