DNA Double‐Strand Breaks in Arctic Char ( Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic
Abstract High levels of organochlorine contaminants (OCs) have been found in arctic char ( Salvelinus alpinus ) from Lake Ellasjøen, Bjørnøya (Norwegian Arctic). The aim of the present study was to investigate the potential genotoxic effect of environmental organochlorine contaminant exposure in arc...
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crwiley:10.1002/etc.4546 2024-06-02T08:00:19+00:00 DNA Double‐Strand Breaks in Arctic Char ( Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic Neerland, Eirik D. Bytingsvik, Jenny Nikiforov, Vladimir A. Evenset, Anita Krøkje, Åse 2019 http://dx.doi.org/10.1002/etc.4546 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.4546 https://onlinelibrary.wiley.com/doi/pdf/10.1002/etc.4546 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/etc.4546 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.4546 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental Toxicology and Chemistry volume 38, issue 11, page 2405-2413 ISSN 0730-7268 1552-8618 journal-article 2019 crwiley https://doi.org/10.1002/etc.4546 2024-05-03T11:53:33Z Abstract High levels of organochlorine contaminants (OCs) have been found in arctic char ( Salvelinus alpinus ) from Lake Ellasjøen, Bjørnøya (Norwegian Arctic). The aim of the present study was to investigate the potential genotoxic effect of environmental organochlorine contaminant exposure in arctic char from Ellasjøen compared with arctic char from the low‐contaminated Lake Laksvatn nearby. Blood was analyzed using agarose gel electrophoresis and image data analysis to quantify the fraction of total DNA that migrated into the gel (DNA‐FTM) as a relative measure of DNA double‐strand breaks (DSBs). Analysis by GC‐MS of muscle samples showed an average 43 times higher concentration of ΣOCs in arctic char from Ellasjøen ( n = 18) compared with Laksvatn char ( n = 21). Char from Lake Ellasjøen had a much higher frequency of DSBs, as measured by DNA‐FTM, than char from Lake Laksvatn. Principal component analysis and multiple linear regressions show that there was a significant positive relationship between DSBs and levels of organochlorine contaminants in the char. In addition, DSBs were less frequent in reproductively mature char than in immature char. The results suggest that organochlorine contaminants are genotoxic to arctic char. Environ Toxicol Chem 2019;38:2405–2413. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC Article in Journal/Newspaper Arctic Bjørnøya Bjørnøya Salvelinus alpinus Wiley Online Library Arctic Bjørnøya ENVELOPE(-67.250,-67.250,-68.151,-68.151) Ellasjøen ENVELOPE(19.031,19.031,74.388,74.388) Laksvatn ENVELOPE(19.360,19.360,69.361,69.361) Environmental Toxicology and Chemistry 38 11 2405 2413 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract High levels of organochlorine contaminants (OCs) have been found in arctic char ( Salvelinus alpinus ) from Lake Ellasjøen, Bjørnøya (Norwegian Arctic). The aim of the present study was to investigate the potential genotoxic effect of environmental organochlorine contaminant exposure in arctic char from Ellasjøen compared with arctic char from the low‐contaminated Lake Laksvatn nearby. Blood was analyzed using agarose gel electrophoresis and image data analysis to quantify the fraction of total DNA that migrated into the gel (DNA‐FTM) as a relative measure of DNA double‐strand breaks (DSBs). Analysis by GC‐MS of muscle samples showed an average 43 times higher concentration of ΣOCs in arctic char from Ellasjøen ( n = 18) compared with Laksvatn char ( n = 21). Char from Lake Ellasjøen had a much higher frequency of DSBs, as measured by DNA‐FTM, than char from Lake Laksvatn. Principal component analysis and multiple linear regressions show that there was a significant positive relationship between DSBs and levels of organochlorine contaminants in the char. In addition, DSBs were less frequent in reproductively mature char than in immature char. The results suggest that organochlorine contaminants are genotoxic to arctic char. Environ Toxicol Chem 2019;38:2405–2413. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC |
format |
Article in Journal/Newspaper |
author |
Neerland, Eirik D. Bytingsvik, Jenny Nikiforov, Vladimir A. Evenset, Anita Krøkje, Åse |
spellingShingle |
Neerland, Eirik D. Bytingsvik, Jenny Nikiforov, Vladimir A. Evenset, Anita Krøkje, Åse DNA Double‐Strand Breaks in Arctic Char ( Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
author_facet |
Neerland, Eirik D. Bytingsvik, Jenny Nikiforov, Vladimir A. Evenset, Anita Krøkje, Åse |
author_sort |
Neerland, Eirik D. |
title |
DNA Double‐Strand Breaks in Arctic Char ( Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_short |
DNA Double‐Strand Breaks in Arctic Char ( Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_full |
DNA Double‐Strand Breaks in Arctic Char ( Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_fullStr |
DNA Double‐Strand Breaks in Arctic Char ( Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_full_unstemmed |
DNA Double‐Strand Breaks in Arctic Char ( Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_sort |
dna double‐strand breaks in arctic char ( salvelinus alpinus) from bjørnøya in the norwegian arctic |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1002/etc.4546 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fetc.4546 https://onlinelibrary.wiley.com/doi/pdf/10.1002/etc.4546 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/etc.4546 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1002/etc.4546 |
long_lat |
ENVELOPE(-67.250,-67.250,-68.151,-68.151) ENVELOPE(19.031,19.031,74.388,74.388) ENVELOPE(19.360,19.360,69.361,69.361) |
geographic |
Arctic Bjørnøya Ellasjøen Laksvatn |
geographic_facet |
Arctic Bjørnøya Ellasjøen Laksvatn |
genre |
Arctic Bjørnøya Bjørnøya Salvelinus alpinus |
genre_facet |
Arctic Bjørnøya Bjørnøya Salvelinus alpinus |
op_source |
Environmental Toxicology and Chemistry volume 38, issue 11, page 2405-2413 ISSN 0730-7268 1552-8618 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/etc.4546 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
38 |
container_issue |
11 |
container_start_page |
2405 |
op_container_end_page |
2413 |
_version_ |
1800744313239371776 |