Trapping and imaging of micron‐sized embryos using dielectrophoresis

• Published in: ELECTROPHORESIS
• Main Authors: Khoshmanesh, Khashayar, Kiss, Nimrod, Nahavandi, Saeid, Evans, Clive W., Cooper, Jonathan M., Williams, David E., Wlodkowic, Donald
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2011
• Subjects: Antarctic; Antarc*
• Online Access: http://dx.doi.org/10.1002/elps.201100160; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Felps.201100160

Abstract Development of dielectrophoretic (DEP) arrays for real‐time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi . Ellipsoid single‐shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof‐of‐concept application of chip‐based technologies for the analysis of individual embryos trapped under DEP force.