Trapping and imaging of micron‐sized embryos using dielectrophoresis

Abstract Development of dielectrophoretic (DEP) arrays for real‐time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi . Ellipsoid single‐shell model was also applied to study t...

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Bibliographic Details
Published in:ELECTROPHORESIS
Main Authors: Khoshmanesh, Khashayar, Kiss, Nimrod, Nahavandi, Saeid, Evans, Clive W., Cooper, Jonathan M., Williams, David E., Wlodkowic, Donald
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2011
Subjects:
Antarctic
Antarc*
Online Access:http://dx.doi.org/10.1002/elps.201100160
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Felps.201100160
Description
Summary:Abstract Development of dielectrophoretic (DEP) arrays for real‐time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi . Ellipsoid single‐shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof‐of‐concept application of chip‐based technologies for the analysis of individual embryos trapped under DEP force.

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