A proteomic analysis of Psychrobacter articus 273‐4 adaptation to low temperature and salinity using a 2‐D liquid mapping approach
Abstract Psychrobacter 273‐4 was isolated from a 20 000–40 000–year‐old Siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. To explore how 273‐4 survives in the permafrost environment, proteins in four 273‐4 samples cultured at 4 and 22°C in me...
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crwiley:10.1002/elps.200600173 2024-09-09T20:03:10+00:00 A proteomic analysis of Psychrobacter articus 273‐4 adaptation to low temperature and salinity using a 2‐D liquid mapping approach Zheng, Suping Ponder, Monica A. Shih, Janice Yang Jen Tiedje, James M. Thomashow, Michael F. Lubman, David M. 2007 http://dx.doi.org/10.1002/elps.200600173 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Felps.200600173 https://onlinelibrary.wiley.com/doi/full/10.1002/elps.200600173 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor ELECTROPHORESIS volume 28, issue 3, page 467-488 ISSN 0173-0835 1522-2683 journal-article 2007 crwiley https://doi.org/10.1002/elps.200600173 2024-08-13T04:18:44Z Abstract Psychrobacter 273‐4 was isolated from a 20 000–40 000–year‐old Siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. To explore how 273‐4 survives in the permafrost environment, proteins in four 273‐4 samples cultured at 4 and 22°C in media with and without 5% sodium chloride were profiled and comparatively studied using 2‐D HPLC and MS. The method used herein involved fractionation via a pH gradient using chromatofocusing followed by nonporous silica (NPS) RP‐HPLC and on‐line electrospray mass mapping. It was observed that 33 proteins were involved in the adaptation to low temperature in the cells grown in the nonsaline media while there were only 14 proteins involved in the saline media. There were 45 proteins observed differentially expressed in response to salt at 22°C while there were 22 proteins at 4°C. In addition, 5% NaCl and 4°C showed a combination effect on protein expression. A total of 56 proteins involved in the adaptation to low temperature and salt were identified using MS and database searching. The differentially expressed proteins were classified into different functional categories where the response of the regulation system to stress appears to be very elaborate. The evidence shows that the adaptation of 273‐4 is based primarily on the control of translation and transcription, the synthesis of proteins (chaperones) to facilitate RNA and protein folding, and the regulation of metabolic pathways. Article in Journal/Newspaper permafrost Wiley Online Library ELECTROPHORESIS 28 3 467 488 |
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Wiley Online Library |
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English |
description |
Abstract Psychrobacter 273‐4 was isolated from a 20 000–40 000–year‐old Siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. To explore how 273‐4 survives in the permafrost environment, proteins in four 273‐4 samples cultured at 4 and 22°C in media with and without 5% sodium chloride were profiled and comparatively studied using 2‐D HPLC and MS. The method used herein involved fractionation via a pH gradient using chromatofocusing followed by nonporous silica (NPS) RP‐HPLC and on‐line electrospray mass mapping. It was observed that 33 proteins were involved in the adaptation to low temperature in the cells grown in the nonsaline media while there were only 14 proteins involved in the saline media. There were 45 proteins observed differentially expressed in response to salt at 22°C while there were 22 proteins at 4°C. In addition, 5% NaCl and 4°C showed a combination effect on protein expression. A total of 56 proteins involved in the adaptation to low temperature and salt were identified using MS and database searching. The differentially expressed proteins were classified into different functional categories where the response of the regulation system to stress appears to be very elaborate. The evidence shows that the adaptation of 273‐4 is based primarily on the control of translation and transcription, the synthesis of proteins (chaperones) to facilitate RNA and protein folding, and the regulation of metabolic pathways. |
format |
Article in Journal/Newspaper |
author |
Zheng, Suping Ponder, Monica A. Shih, Janice Yang Jen Tiedje, James M. Thomashow, Michael F. Lubman, David M. |
spellingShingle |
Zheng, Suping Ponder, Monica A. Shih, Janice Yang Jen Tiedje, James M. Thomashow, Michael F. Lubman, David M. A proteomic analysis of Psychrobacter articus 273‐4 adaptation to low temperature and salinity using a 2‐D liquid mapping approach |
author_facet |
Zheng, Suping Ponder, Monica A. Shih, Janice Yang Jen Tiedje, James M. Thomashow, Michael F. Lubman, David M. |
author_sort |
Zheng, Suping |
title |
A proteomic analysis of Psychrobacter articus 273‐4 adaptation to low temperature and salinity using a 2‐D liquid mapping approach |
title_short |
A proteomic analysis of Psychrobacter articus 273‐4 adaptation to low temperature and salinity using a 2‐D liquid mapping approach |
title_full |
A proteomic analysis of Psychrobacter articus 273‐4 adaptation to low temperature and salinity using a 2‐D liquid mapping approach |
title_fullStr |
A proteomic analysis of Psychrobacter articus 273‐4 adaptation to low temperature and salinity using a 2‐D liquid mapping approach |
title_full_unstemmed |
A proteomic analysis of Psychrobacter articus 273‐4 adaptation to low temperature and salinity using a 2‐D liquid mapping approach |
title_sort |
proteomic analysis of psychrobacter articus 273‐4 adaptation to low temperature and salinity using a 2‐d liquid mapping approach |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1002/elps.200600173 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Felps.200600173 https://onlinelibrary.wiley.com/doi/full/10.1002/elps.200600173 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
ELECTROPHORESIS volume 28, issue 3, page 467-488 ISSN 0173-0835 1522-2683 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/elps.200600173 |
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ELECTROPHORESIS |
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28 |
container_issue |
3 |
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467 |
op_container_end_page |
488 |
_version_ |
1809935129847529472 |