Tissue distribution of selected micro RNA in Atlantic salmon
microRNAs (miRNAs) are small non‐coding RNAs that regulate numerous fundamental biological functions. So far miRNAs have only been identified in few commercially important fish species. Similarly to messenger RNA (mRNA) expression analysis, miRNA qPCR analysis requires thoroughly investigated endoge...
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crwiley:10.1002/ejlt.201200428 2024-06-02T08:03:25+00:00 Tissue distribution of selected micro RNA in Atlantic salmon Trattner, Sofia Vestergren, AnnaLotta Schiller 2013 http://dx.doi.org/10.1002/ejlt.201200428 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fejlt.201200428 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ejlt.201200428 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ejlt.201200428 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor European Journal of Lipid Science and Technology volume 115, issue 12, page 1348-1356 ISSN 1438-7697 1438-9312 journal-article 2013 crwiley https://doi.org/10.1002/ejlt.201200428 2024-05-03T10:37:03Z microRNAs (miRNAs) are small non‐coding RNAs that regulate numerous fundamental biological functions. So far miRNAs have only been identified in few commercially important fish species. Similarly to messenger RNA (mRNA) expression analysis, miRNA qPCR analysis requires thoroughly investigated endogenous controls. In this paper, we have in a pilot study investigated miRNAs in Atlantic salmon (liver, heart, brain, kidney, spleen, intestine, gill, white, and red muscle) by qPCR analysis. The expression of seven putative endogenous control genes (ssa‐let‐7a, ssa‐miR‐16a, ssa‐miR‐16b, ssa‐miR‐194a, ssa‐miR‐22a, ssa‐miR22b, and ssa‐miR‐27c) was examined with regard to their tissue distribution and use as endogenous controls microRNA expression studies. The candidates for endogenous controls were selected as they had been reported by others to be evenly expressed among tissues. The most suitable endogenous control was miR‐27c in this study. Further the expression of ssa‐miR 26b, ssa‐miR‐92a‐1, ssa‐miR‐122, ssa‐miR‐722, ssa‐miR‐21‐1, and ssa‐miR‐143‐1 were investigated and tissue specific expression of all miRNAs except ssa‐miR194a were found. The expression of sss‐miR‐122 and ssa‐miR‐722 were specific for liver, ssa‐miR‐26b mostly expressed in brain and ssa‐miR143‐1 mostly expressed in gill and kidney. This study has provided information of endogenous control genes and has also identified tissue specific expression of miRNAs in Atlantic salmon. Further investigation of endogenous control genes target genes in response to treatment would increase the knowledge of the role of miRNAs in Atlantic salmon. Article in Journal/Newspaper Atlantic salmon Wiley Online Library European Journal of Lipid Science and Technology 115 12 1348 1356 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
microRNAs (miRNAs) are small non‐coding RNAs that regulate numerous fundamental biological functions. So far miRNAs have only been identified in few commercially important fish species. Similarly to messenger RNA (mRNA) expression analysis, miRNA qPCR analysis requires thoroughly investigated endogenous controls. In this paper, we have in a pilot study investigated miRNAs in Atlantic salmon (liver, heart, brain, kidney, spleen, intestine, gill, white, and red muscle) by qPCR analysis. The expression of seven putative endogenous control genes (ssa‐let‐7a, ssa‐miR‐16a, ssa‐miR‐16b, ssa‐miR‐194a, ssa‐miR‐22a, ssa‐miR22b, and ssa‐miR‐27c) was examined with regard to their tissue distribution and use as endogenous controls microRNA expression studies. The candidates for endogenous controls were selected as they had been reported by others to be evenly expressed among tissues. The most suitable endogenous control was miR‐27c in this study. Further the expression of ssa‐miR 26b, ssa‐miR‐92a‐1, ssa‐miR‐122, ssa‐miR‐722, ssa‐miR‐21‐1, and ssa‐miR‐143‐1 were investigated and tissue specific expression of all miRNAs except ssa‐miR194a were found. The expression of sss‐miR‐122 and ssa‐miR‐722 were specific for liver, ssa‐miR‐26b mostly expressed in brain and ssa‐miR143‐1 mostly expressed in gill and kidney. This study has provided information of endogenous control genes and has also identified tissue specific expression of miRNAs in Atlantic salmon. Further investigation of endogenous control genes target genes in response to treatment would increase the knowledge of the role of miRNAs in Atlantic salmon. |
format |
Article in Journal/Newspaper |
author |
Trattner, Sofia Vestergren, AnnaLotta Schiller |
spellingShingle |
Trattner, Sofia Vestergren, AnnaLotta Schiller Tissue distribution of selected micro RNA in Atlantic salmon |
author_facet |
Trattner, Sofia Vestergren, AnnaLotta Schiller |
author_sort |
Trattner, Sofia |
title |
Tissue distribution of selected micro RNA in Atlantic salmon |
title_short |
Tissue distribution of selected micro RNA in Atlantic salmon |
title_full |
Tissue distribution of selected micro RNA in Atlantic salmon |
title_fullStr |
Tissue distribution of selected micro RNA in Atlantic salmon |
title_full_unstemmed |
Tissue distribution of selected micro RNA in Atlantic salmon |
title_sort |
tissue distribution of selected micro rna in atlantic salmon |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1002/ejlt.201200428 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fejlt.201200428 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ejlt.201200428 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ejlt.201200428 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
European Journal of Lipid Science and Technology volume 115, issue 12, page 1348-1356 ISSN 1438-7697 1438-9312 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/ejlt.201200428 |
container_title |
European Journal of Lipid Science and Technology |
container_volume |
115 |
container_issue |
12 |
container_start_page |
1348 |
op_container_end_page |
1356 |
_version_ |
1800747944166555648 |