New highly robust divinyl benzene/acrylate polymer for immobilization of lipase CALB
Abstract Candida antartica lipase B (CALB) is the most versatile biocatalyst and is, therefore, widely used in industry. Its advantages range from thermostability, stereoselectivity, and activity in organic solvents to the acceptance of nucleophiles other than water. The actual standard for industri...
Published in: | European Journal of Lipid Science and Technology |
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crwiley:10.1002/ejlt.201200419 2024-06-23T07:48:04+00:00 New highly robust divinyl benzene/acrylate polymer for immobilization of lipase CALB Basso, Alessandra Froment, Luc Hesseler, Martin Serban, Simona 2013 http://dx.doi.org/10.1002/ejlt.201200419 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fejlt.201200419 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ejlt.201200419 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ejlt.201200419 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor European Journal of Lipid Science and Technology volume 115, issue 4, page 468-472 ISSN 1438-7697 1438-9312 journal-article 2013 crwiley https://doi.org/10.1002/ejlt.201200419 2024-06-04T06:45:01Z Abstract Candida antartica lipase B (CALB) is the most versatile biocatalyst and is, therefore, widely used in industry. Its advantages range from thermostability, stereoselectivity, and activity in organic solvents to the acceptance of nucleophiles other than water. The actual standard for industrial applications of CALB is the immobilized enzyme on Lewatit VP OC 1600 and is marketed under the name Novozym® 435. A new acrylic enzyme carrier (ECR1030) for the immobilization by adsorption of CALB and for use in batch or column reactors has been developed. The resin shows an activity of >9000 PLU/g Dry , which is similar to Novozym® 435. Protein loading was 24.4 mg/g Wet, and immobilizations yields higher than 90% were obtained. Unlike the resin used for the production of Novozym® 435, ECR1030 possesses high mechanical stability and is therefore more suitable for applications that expose the polymers to high mechanical stress, such as multiple reuses in stirred reactors. Practical applications: Immobilized CALB is the biocatalyst used in the widest amount of industrial applications due to its broad selectivity and stability. It can be used for the manufacture of specialty chemicals such as surfactants, amides, chiral amines, alcohols, biodiesel, and polyesters. Its industrial use is in stirred tanks or packed columns, so high mechanical stability is needed together with high activity and sustainable costs. Article in Journal/Newspaper antartic* Wiley Online Library European Journal of Lipid Science and Technology 115 4 468 472 |
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Wiley Online Library |
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English |
description |
Abstract Candida antartica lipase B (CALB) is the most versatile biocatalyst and is, therefore, widely used in industry. Its advantages range from thermostability, stereoselectivity, and activity in organic solvents to the acceptance of nucleophiles other than water. The actual standard for industrial applications of CALB is the immobilized enzyme on Lewatit VP OC 1600 and is marketed under the name Novozym® 435. A new acrylic enzyme carrier (ECR1030) for the immobilization by adsorption of CALB and for use in batch or column reactors has been developed. The resin shows an activity of >9000 PLU/g Dry , which is similar to Novozym® 435. Protein loading was 24.4 mg/g Wet, and immobilizations yields higher than 90% were obtained. Unlike the resin used for the production of Novozym® 435, ECR1030 possesses high mechanical stability and is therefore more suitable for applications that expose the polymers to high mechanical stress, such as multiple reuses in stirred reactors. Practical applications: Immobilized CALB is the biocatalyst used in the widest amount of industrial applications due to its broad selectivity and stability. It can be used for the manufacture of specialty chemicals such as surfactants, amides, chiral amines, alcohols, biodiesel, and polyesters. Its industrial use is in stirred tanks or packed columns, so high mechanical stability is needed together with high activity and sustainable costs. |
format |
Article in Journal/Newspaper |
author |
Basso, Alessandra Froment, Luc Hesseler, Martin Serban, Simona |
spellingShingle |
Basso, Alessandra Froment, Luc Hesseler, Martin Serban, Simona New highly robust divinyl benzene/acrylate polymer for immobilization of lipase CALB |
author_facet |
Basso, Alessandra Froment, Luc Hesseler, Martin Serban, Simona |
author_sort |
Basso, Alessandra |
title |
New highly robust divinyl benzene/acrylate polymer for immobilization of lipase CALB |
title_short |
New highly robust divinyl benzene/acrylate polymer for immobilization of lipase CALB |
title_full |
New highly robust divinyl benzene/acrylate polymer for immobilization of lipase CALB |
title_fullStr |
New highly robust divinyl benzene/acrylate polymer for immobilization of lipase CALB |
title_full_unstemmed |
New highly robust divinyl benzene/acrylate polymer for immobilization of lipase CALB |
title_sort |
new highly robust divinyl benzene/acrylate polymer for immobilization of lipase calb |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1002/ejlt.201200419 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fejlt.201200419 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ejlt.201200419 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ejlt.201200419 |
genre |
antartic* |
genre_facet |
antartic* |
op_source |
European Journal of Lipid Science and Technology volume 115, issue 4, page 468-472 ISSN 1438-7697 1438-9312 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/ejlt.201200419 |
container_title |
European Journal of Lipid Science and Technology |
container_volume |
115 |
container_issue |
4 |
container_start_page |
468 |
op_container_end_page |
472 |
_version_ |
1802638483965083648 |