Synthesis of 2‐monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases
Abstract Commercial immobilized lipases were used for the synthesis of 2‐monoglycerides (2‐MG) by alcoholysis of palm and tuna oils with ethanol in organic solvents. Several parameters were studied, i.e. , the type of immobilized lipases, water activity, type of solvents and temperatures. The optimu...
Published in: | European Journal of Lipid Science and Technology |
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Online Access: | http://dx.doi.org/10.1002/ejlt.200390019 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ejlt.200390019 |
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crwiley:10.1002/ejlt.200390019 2024-06-02T07:58:41+00:00 Synthesis of 2‐monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases Wongsakul, Sirirung Prasertsan, Poonsuk Bornscheuer, Uwe T. H‐Kittikun, Aran 2003 http://dx.doi.org/10.1002/ejlt.200390019 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ejlt.200390019 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor European Journal of Lipid Science and Technology volume 105, issue 2, page 68-73 ISSN 1438-7697 1438-9312 journal-article 2003 crwiley https://doi.org/10.1002/ejlt.200390019 2024-05-03T11:03:21Z Abstract Commercial immobilized lipases were used for the synthesis of 2‐monoglycerides (2‐MG) by alcoholysis of palm and tuna oils with ethanol in organic solvents. Several parameters were studied, i.e. , the type of immobilized lipases, water activity, type of solvents and temperatures. The optimum conditions for alcoholysis of tuna oil were at a water activity of 0.43 and a temperature of 60 °C in methyl‐ tert ‐butyl ether for ∼12 h. Although immobilized lipase preparations from Pseudomonas sp. and Candida antarctica fraction B are not 1, 3‐regiospecific enzymes, they were considered to be more suitable for the production of 2‐MG by the alcoholysis of tuna oil than the 1, 3‐regiospecific lipases (Lipozyme RM IM from Rhizomucor miehei and lipase D from Rhizopus delemar ). With Pseudomonas sp. lipase a yield of up to 81% 2‐MG containing 80% PUFA (poly‐unsaturated fatty acids) from tuna oil was achieved. The optimum conditions for alcoholysis of palm oil were similar as these of tuna oil alcoholysis. However, lipase D immobilized on Accurel EP100 was used as catalyst at 40 °C with shorter reaction times (<12 h). This lead to a yield of ∼60% 2‐MG containing 55.0‐55.7% oleic acid and 18.7‐21.0% linoleic acid. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library European Journal of Lipid Science and Technology 105 2 68 73 |
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Wiley Online Library |
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English |
description |
Abstract Commercial immobilized lipases were used for the synthesis of 2‐monoglycerides (2‐MG) by alcoholysis of palm and tuna oils with ethanol in organic solvents. Several parameters were studied, i.e. , the type of immobilized lipases, water activity, type of solvents and temperatures. The optimum conditions for alcoholysis of tuna oil were at a water activity of 0.43 and a temperature of 60 °C in methyl‐ tert ‐butyl ether for ∼12 h. Although immobilized lipase preparations from Pseudomonas sp. and Candida antarctica fraction B are not 1, 3‐regiospecific enzymes, they were considered to be more suitable for the production of 2‐MG by the alcoholysis of tuna oil than the 1, 3‐regiospecific lipases (Lipozyme RM IM from Rhizomucor miehei and lipase D from Rhizopus delemar ). With Pseudomonas sp. lipase a yield of up to 81% 2‐MG containing 80% PUFA (poly‐unsaturated fatty acids) from tuna oil was achieved. The optimum conditions for alcoholysis of palm oil were similar as these of tuna oil alcoholysis. However, lipase D immobilized on Accurel EP100 was used as catalyst at 40 °C with shorter reaction times (<12 h). This lead to a yield of ∼60% 2‐MG containing 55.0‐55.7% oleic acid and 18.7‐21.0% linoleic acid. |
format |
Article in Journal/Newspaper |
author |
Wongsakul, Sirirung Prasertsan, Poonsuk Bornscheuer, Uwe T. H‐Kittikun, Aran |
spellingShingle |
Wongsakul, Sirirung Prasertsan, Poonsuk Bornscheuer, Uwe T. H‐Kittikun, Aran Synthesis of 2‐monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases |
author_facet |
Wongsakul, Sirirung Prasertsan, Poonsuk Bornscheuer, Uwe T. H‐Kittikun, Aran |
author_sort |
Wongsakul, Sirirung |
title |
Synthesis of 2‐monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases |
title_short |
Synthesis of 2‐monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases |
title_full |
Synthesis of 2‐monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases |
title_fullStr |
Synthesis of 2‐monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases |
title_full_unstemmed |
Synthesis of 2‐monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases |
title_sort |
synthesis of 2‐monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1002/ejlt.200390019 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ejlt.200390019 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
European Journal of Lipid Science and Technology volume 105, issue 2, page 68-73 ISSN 1438-7697 1438-9312 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/ejlt.200390019 |
container_title |
European Journal of Lipid Science and Technology |
container_volume |
105 |
container_issue |
2 |
container_start_page |
68 |
op_container_end_page |
73 |
_version_ |
1800742139502526464 |