Immunoglobulin heavy chain cDNA from the teleost Atlantic cod ( Gadus morhua L.): nucleotide sequences of secretory and membrane form show an unusual splicing pattern
Abstract Rabbit antibodies to Atlantic cod ( Gadus morhua L.) immunoglobulin were affinity purified and used to screen cDNA libraries from spleen and head kidney mRNA. cDNA clones for both the secretory and membrane‐bound heavy (H) chain were isolated, the nucleotide and deduced amino acid sequences...
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crwiley:10.1002/eji.1830211219 2024-10-13T14:05:55+00:00 Immunoglobulin heavy chain cDNA from the teleost Atlantic cod ( Gadus morhua L.): nucleotide sequences of secretory and membrane form show an unusual splicing pattern Bengtén, Eva Leanderson, Tomas Pilström, Lars 1991 http://dx.doi.org/10.1002/eji.1830211219 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Feji.1830211219 https://onlinelibrary.wiley.com/doi/pdf/10.1002/eji.1830211219 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor European Journal of Immunology volume 21, issue 12, page 3027-3033 ISSN 0014-2980 1521-4141 journal-article 1991 crwiley https://doi.org/10.1002/eji.1830211219 2024-09-17T04:47:14Z Abstract Rabbit antibodies to Atlantic cod ( Gadus morhua L.) immunoglobulin were affinity purified and used to screen cDNA libraries from spleen and head kidney mRNA. cDNA clones for both the secretory and membrane‐bound heavy (H) chain were isolated, the nucleotide and deduced amino acid sequences of which are reported here. Comparisons of the cod secretory H chain amino acid sequence show 24%, 27%, 30% identity to the μ chain of Mus, Xenopus and Ictalurus , respectively. The highest degree of identity was observed in the C H 4 domain. The cDNA encoding the transmembrane form shows a novel splicing pattern where the TM1 exon is spliced directly onto the C H 3 domain and not to the C H 4 domain as in other animal groups. Southern blot analyses with V H and C probes on genomic DNA from cod erythrocytes indicate that there is a unique C gene but several V genes in the cod immunoglobulin H chain locus. Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library European Journal of Immunology 21 12 3027 3033 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Rabbit antibodies to Atlantic cod ( Gadus morhua L.) immunoglobulin were affinity purified and used to screen cDNA libraries from spleen and head kidney mRNA. cDNA clones for both the secretory and membrane‐bound heavy (H) chain were isolated, the nucleotide and deduced amino acid sequences of which are reported here. Comparisons of the cod secretory H chain amino acid sequence show 24%, 27%, 30% identity to the μ chain of Mus, Xenopus and Ictalurus , respectively. The highest degree of identity was observed in the C H 4 domain. The cDNA encoding the transmembrane form shows a novel splicing pattern where the TM1 exon is spliced directly onto the C H 3 domain and not to the C H 4 domain as in other animal groups. Southern blot analyses with V H and C probes on genomic DNA from cod erythrocytes indicate that there is a unique C gene but several V genes in the cod immunoglobulin H chain locus. |
format |
Article in Journal/Newspaper |
author |
Bengtén, Eva Leanderson, Tomas Pilström, Lars |
spellingShingle |
Bengtén, Eva Leanderson, Tomas Pilström, Lars Immunoglobulin heavy chain cDNA from the teleost Atlantic cod ( Gadus morhua L.): nucleotide sequences of secretory and membrane form show an unusual splicing pattern |
author_facet |
Bengtén, Eva Leanderson, Tomas Pilström, Lars |
author_sort |
Bengtén, Eva |
title |
Immunoglobulin heavy chain cDNA from the teleost Atlantic cod ( Gadus morhua L.): nucleotide sequences of secretory and membrane form show an unusual splicing pattern |
title_short |
Immunoglobulin heavy chain cDNA from the teleost Atlantic cod ( Gadus morhua L.): nucleotide sequences of secretory and membrane form show an unusual splicing pattern |
title_full |
Immunoglobulin heavy chain cDNA from the teleost Atlantic cod ( Gadus morhua L.): nucleotide sequences of secretory and membrane form show an unusual splicing pattern |
title_fullStr |
Immunoglobulin heavy chain cDNA from the teleost Atlantic cod ( Gadus morhua L.): nucleotide sequences of secretory and membrane form show an unusual splicing pattern |
title_full_unstemmed |
Immunoglobulin heavy chain cDNA from the teleost Atlantic cod ( Gadus morhua L.): nucleotide sequences of secretory and membrane form show an unusual splicing pattern |
title_sort |
immunoglobulin heavy chain cdna from the teleost atlantic cod ( gadus morhua l.): nucleotide sequences of secretory and membrane form show an unusual splicing pattern |
publisher |
Wiley |
publishDate |
1991 |
url |
http://dx.doi.org/10.1002/eji.1830211219 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Feji.1830211219 https://onlinelibrary.wiley.com/doi/pdf/10.1002/eji.1830211219 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
European Journal of Immunology volume 21, issue 12, page 3027-3033 ISSN 0014-2980 1521-4141 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/eji.1830211219 |
container_title |
European Journal of Immunology |
container_volume |
21 |
container_issue |
12 |
container_start_page |
3027 |
op_container_end_page |
3033 |
_version_ |
1812811954371166208 |