Antigen processing by endosomal proteases determines which sites of sperm‐whale myoglobin are eventually recognized by T cells

• Published in: European Journal of Immunology
• Main Authors: Van Noort, Johannes M., Boon, Jacqueline, Van der Drift, Alfons C. M., Wagenaar, Josée P. A., Boots, Annemieke M. H., Boog, Claire J. P.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1991
• Subjects: Sperm whale
• Online Access: http://dx.doi.org/10.1002/eji.1830210904; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Feji.1830210904; https://onlinelibrary.wiley.com/doi/pdf/10.1002/eji.1830210904

Abstract This study reports an identification of the major processing products of an exogenous protein antigen, viz. sperm‐whale myoglobin, as obtained after cell‐free processing with partially purified macrophage endosomes. It is demonstrated that such a system yields fragments that are indistinguishable by high performance liquid chromatography analysis from those generated after uptake of myoglobin inside live macrophages. The concerted action of the endosomal proteases cathepsin D and cathepsin B can account for nearly all cleavages observed. Cathepsin D appears to be mainly responsible for the initial cleavage of myoglobin, while cathepsin B catalyzes the C‐terminal trimming of initially released fragments. The fragments released by cathepsin D contain most, if not all, major epitopes for murine myoglobin‐specific helper T cells. Interestingly, each known T cell epitope of myoglobin is located at the very N terminus of a different myoglobin fragment released upon processing. In order to explain this correspondence, noted also in several other protein antigens, a structural relationship is proposed between antigen processing by cathepsin D and antigen recognition by major histocompatibility complex (MHC) class II products. As is demonstrated here, this relationship may be used as a predictive tool for the identification of MHC‐binding sequences as well as of T cell epitopes in their naturally occurring form.