Effect of liposomes on lymphocytes: Induction of proliferation of B lymphocytes and potentiation of the cytotoxic response of T lymphocytes to alloantigens
Abstract Liposomes of certain lipid composition prepared by the detergent removal method (Brunner, J. et al., Biochim. Biophys. Acta 1976. 455 : 322) induced the proliferation of spleen cells from different mouse strains. Spleen cell populations enriched in B lymphocytes and those obtained from nude...
Published in: | European Journal of Immunology |
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Language: | English |
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1986
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Online Access: | http://dx.doi.org/10.1002/eji.1830160613 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Feji.1830160613 https://onlinelibrary.wiley.com/doi/pdf/10.1002/eji.1830160613 |
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crwiley:10.1002/eji.1830160613 2024-06-02T08:05:47+00:00 Effect of liposomes on lymphocytes: Induction of proliferation of B lymphocytes and potentiation of the cytotoxic response of T lymphocytes to alloantigens Grover, Amita Sundharadas, G. 1986 http://dx.doi.org/10.1002/eji.1830160613 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Feji.1830160613 https://onlinelibrary.wiley.com/doi/pdf/10.1002/eji.1830160613 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor European Journal of Immunology volume 16, issue 6, page 665-670 ISSN 0014-2980 1521-4141 journal-article 1986 crwiley https://doi.org/10.1002/eji.1830160613 2024-05-03T11:10:56Z Abstract Liposomes of certain lipid composition prepared by the detergent removal method (Brunner, J. et al., Biochim. Biophys. Acta 1976. 455 : 322) induced the proliferation of spleen cells from different mouse strains. Spleen cell populations enriched in B lymphocytes and those obtained from nude mice were induced to proliferate, whereas spleen cell fractions enriched in T lymphocytes and thymocytes were not. The mitogenic effect of liposomes resembled that of lipopolysaccharide (LPS) and it depended upon their lipid composition. Liposomes prepared from dimyristoyl lecithin (DML), 2:1 dimyristoyl lecithin:cholesterol (DML:C), 2:1 dioleoyl lecithin: cholesterol (DOL:C), and 2:1 egg yolk lecithin:cholesterol (EYL:C) were mitogenic, whereas liposomes prepared from egg yolk lecithin (EYL) alone were not mitogenic for spleen cells. The mitogenic effects of these liposome preparations were in the decreasing order DML > DOL: C ⩾ EYL: C > DML: C > EYL. The results suggest a correlation between the membrane fluidity of liposomes and their mitogenic effect. Although no proliferative response was induced on T lymphocytes, two of these liposomes, DML and EYL: C, had the ability to potentiate the cytotoxic response of T lymphocytes to alloantigens in mixed leukocyte culture. In responder‐stimulator combinations which differed for the H‐2K, H‐2D or the entire H‐2 region, these liposomes potentiated the cytotoxic response significantly. The results suggest that liposomes have an ability to modulate T lymphocyte response. Article in Journal/Newspaper DML Wiley Online Library European Journal of Immunology 16 6 665 670 |
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Abstract Liposomes of certain lipid composition prepared by the detergent removal method (Brunner, J. et al., Biochim. Biophys. Acta 1976. 455 : 322) induced the proliferation of spleen cells from different mouse strains. Spleen cell populations enriched in B lymphocytes and those obtained from nude mice were induced to proliferate, whereas spleen cell fractions enriched in T lymphocytes and thymocytes were not. The mitogenic effect of liposomes resembled that of lipopolysaccharide (LPS) and it depended upon their lipid composition. Liposomes prepared from dimyristoyl lecithin (DML), 2:1 dimyristoyl lecithin:cholesterol (DML:C), 2:1 dioleoyl lecithin: cholesterol (DOL:C), and 2:1 egg yolk lecithin:cholesterol (EYL:C) were mitogenic, whereas liposomes prepared from egg yolk lecithin (EYL) alone were not mitogenic for spleen cells. The mitogenic effects of these liposome preparations were in the decreasing order DML > DOL: C ⩾ EYL: C > DML: C > EYL. The results suggest a correlation between the membrane fluidity of liposomes and their mitogenic effect. Although no proliferative response was induced on T lymphocytes, two of these liposomes, DML and EYL: C, had the ability to potentiate the cytotoxic response of T lymphocytes to alloantigens in mixed leukocyte culture. In responder‐stimulator combinations which differed for the H‐2K, H‐2D or the entire H‐2 region, these liposomes potentiated the cytotoxic response significantly. The results suggest that liposomes have an ability to modulate T lymphocyte response. |
format |
Article in Journal/Newspaper |
author |
Grover, Amita Sundharadas, G. |
spellingShingle |
Grover, Amita Sundharadas, G. Effect of liposomes on lymphocytes: Induction of proliferation of B lymphocytes and potentiation of the cytotoxic response of T lymphocytes to alloantigens |
author_facet |
Grover, Amita Sundharadas, G. |
author_sort |
Grover, Amita |
title |
Effect of liposomes on lymphocytes: Induction of proliferation of B lymphocytes and potentiation of the cytotoxic response of T lymphocytes to alloantigens |
title_short |
Effect of liposomes on lymphocytes: Induction of proliferation of B lymphocytes and potentiation of the cytotoxic response of T lymphocytes to alloantigens |
title_full |
Effect of liposomes on lymphocytes: Induction of proliferation of B lymphocytes and potentiation of the cytotoxic response of T lymphocytes to alloantigens |
title_fullStr |
Effect of liposomes on lymphocytes: Induction of proliferation of B lymphocytes and potentiation of the cytotoxic response of T lymphocytes to alloantigens |
title_full_unstemmed |
Effect of liposomes on lymphocytes: Induction of proliferation of B lymphocytes and potentiation of the cytotoxic response of T lymphocytes to alloantigens |
title_sort |
effect of liposomes on lymphocytes: induction of proliferation of b lymphocytes and potentiation of the cytotoxic response of t lymphocytes to alloantigens |
publisher |
Wiley |
publishDate |
1986 |
url |
http://dx.doi.org/10.1002/eji.1830160613 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Feji.1830160613 https://onlinelibrary.wiley.com/doi/pdf/10.1002/eji.1830160613 |
genre |
DML |
genre_facet |
DML |
op_source |
European Journal of Immunology volume 16, issue 6, page 665-670 ISSN 0014-2980 1521-4141 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/eji.1830160613 |
container_title |
European Journal of Immunology |
container_volume |
16 |
container_issue |
6 |
container_start_page |
665 |
op_container_end_page |
670 |
_version_ |
1800750671474982912 |