Environmental DNA detection of Arctic char ( Salvelinus alpinus) in Irish lakes: Development and application of a species‐specific molecular assay
Abstract Populations of Arctic char ( Salvelinus alpinus ) inhabiting Irish lakes are undergoing a rapid decline. In order to aid monitoring of this species in Irish lakes, a species‐specific qPCR assay was designed and subsequently tested for the detection of Arctic char's environmental DNA (e...
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crwiley:10.1002/edn3.60 2024-06-02T08:01:01+00:00 Environmental DNA detection of Arctic char ( Salvelinus alpinus) in Irish lakes: Development and application of a species‐specific molecular assay Mirimin, Luca Hickey, Aaron Barrett, Dylan DeFaoite, Fergus Boschetti, Simona Venkatesh, Shraveena Graham, Conor T. Department of Agriculture, Forestry and the Marine 2020 http://dx.doi.org/10.1002/edn3.60 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fedn3.60 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.60 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/edn3.60 en eng Wiley http://creativecommons.org/licenses/by-nc/4.0/ Environmental DNA volume 2, issue 2, page 221-233 ISSN 2637-4943 2637-4943 journal-article 2020 crwiley https://doi.org/10.1002/edn3.60 2024-05-03T11:21:30Z Abstract Populations of Arctic char ( Salvelinus alpinus ) inhabiting Irish lakes are undergoing a rapid decline. In order to aid monitoring of this species in Irish lakes, a species‐specific qPCR assay was designed and subsequently tested for the detection of Arctic char's environmental DNA (eDNA) in an indoor mesocosm and in natural habitats (six lakes). Specifically, the mesocosm experiment revealed that sample volume can have significant effects on the application of eDNA approaches, whereby probability of detection depends from proximity to the source organism and volume of water filtered. When tested in the wild, positive amplification of Arctic char eDNA was observed in four of the six lakes sampled, proving that this approach can be successfully implemented in natural conditions. However, findings from the present study also showed that suboptimal sampling strategies can lead to false‐negative results (e.g., failure to detect the species when it is present). This study corroborates findings from other research on eDNA detection of fish species, showing that despite its current limitations, this approach can be a fast, noninvasive, and effective tool in aid to the conservation and management of freshwater fish species. Article in Journal/Newspaper Arctic Salvelinus alpinus Wiley Online Library Arctic Environmental DNA 2 2 221 233 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Populations of Arctic char ( Salvelinus alpinus ) inhabiting Irish lakes are undergoing a rapid decline. In order to aid monitoring of this species in Irish lakes, a species‐specific qPCR assay was designed and subsequently tested for the detection of Arctic char's environmental DNA (eDNA) in an indoor mesocosm and in natural habitats (six lakes). Specifically, the mesocosm experiment revealed that sample volume can have significant effects on the application of eDNA approaches, whereby probability of detection depends from proximity to the source organism and volume of water filtered. When tested in the wild, positive amplification of Arctic char eDNA was observed in four of the six lakes sampled, proving that this approach can be successfully implemented in natural conditions. However, findings from the present study also showed that suboptimal sampling strategies can lead to false‐negative results (e.g., failure to detect the species when it is present). This study corroborates findings from other research on eDNA detection of fish species, showing that despite its current limitations, this approach can be a fast, noninvasive, and effective tool in aid to the conservation and management of freshwater fish species. |
author2 |
Department of Agriculture, Forestry and the Marine |
format |
Article in Journal/Newspaper |
author |
Mirimin, Luca Hickey, Aaron Barrett, Dylan DeFaoite, Fergus Boschetti, Simona Venkatesh, Shraveena Graham, Conor T. |
spellingShingle |
Mirimin, Luca Hickey, Aaron Barrett, Dylan DeFaoite, Fergus Boschetti, Simona Venkatesh, Shraveena Graham, Conor T. Environmental DNA detection of Arctic char ( Salvelinus alpinus) in Irish lakes: Development and application of a species‐specific molecular assay |
author_facet |
Mirimin, Luca Hickey, Aaron Barrett, Dylan DeFaoite, Fergus Boschetti, Simona Venkatesh, Shraveena Graham, Conor T. |
author_sort |
Mirimin, Luca |
title |
Environmental DNA detection of Arctic char ( Salvelinus alpinus) in Irish lakes: Development and application of a species‐specific molecular assay |
title_short |
Environmental DNA detection of Arctic char ( Salvelinus alpinus) in Irish lakes: Development and application of a species‐specific molecular assay |
title_full |
Environmental DNA detection of Arctic char ( Salvelinus alpinus) in Irish lakes: Development and application of a species‐specific molecular assay |
title_fullStr |
Environmental DNA detection of Arctic char ( Salvelinus alpinus) in Irish lakes: Development and application of a species‐specific molecular assay |
title_full_unstemmed |
Environmental DNA detection of Arctic char ( Salvelinus alpinus) in Irish lakes: Development and application of a species‐specific molecular assay |
title_sort |
environmental dna detection of arctic char ( salvelinus alpinus) in irish lakes: development and application of a species‐specific molecular assay |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1002/edn3.60 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fedn3.60 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.60 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/edn3.60 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Salvelinus alpinus |
genre_facet |
Arctic Salvelinus alpinus |
op_source |
Environmental DNA volume 2, issue 2, page 221-233 ISSN 2637-4943 2637-4943 |
op_rights |
http://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.1002/edn3.60 |
container_title |
Environmental DNA |
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2 |
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2 |
container_start_page |
221 |
op_container_end_page |
233 |
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1800745258211868672 |