Detecting the invisible through DNA metabarcoding: The role of gelatinous taxa in the diet of two demersal Antarctic key stone fish species (Notothenioidei)
Abstract Gelatinous zooplankton (GZP), i.e., ctenophores, cnidarian medusae, chaetognaths, appendicularians and salps, are considered climate change winners. This becomes particularly obvious in the Southern Ocean, which has undergone a significant shift from a krill‐based to a salp‐based ecosystem...
Published in: | Environmental DNA |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2024
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/edn3.561 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.561 |
id |
crwiley:10.1002/edn3.561 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/edn3.561 2024-09-09T19:05:21+00:00 Detecting the invisible through DNA metabarcoding: The role of gelatinous taxa in the diet of two demersal Antarctic key stone fish species (Notothenioidei) Ruiz, Micaela B. Moreira, Eugenia Novillo, Manuel Neuhaus, Stefan Leese, Florian Havermans, Charlotte Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research Deutsche Forschungsgemeinschaft Fondo para la Investigación Científica y Tecnológica 2024 http://dx.doi.org/10.1002/edn3.561 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.561 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental DNA volume 6, issue 3 ISSN 2637-4943 2637-4943 journal-article 2024 crwiley https://doi.org/10.1002/edn3.561 2024-07-02T04:12:44Z Abstract Gelatinous zooplankton (GZP), i.e., ctenophores, cnidarian medusae, chaetognaths, appendicularians and salps, are considered climate change winners. This becomes particularly obvious in the Southern Ocean, which has undergone a significant shift from a krill‐based to a salp‐based ecosystem over the last decades. A better knowledge on the role of gelatinous invertebrates as prey is needed to predict the impact of such a gelatinous shift. Until recently, GZP was considered as a “trophic dead end”. However, their true importance in diets has remained unresolved due to the rapid digestion of their watery and soft tissues in predators' stomachs. In this study, we want to validate the paradigm shift from GZP being considered as “survival food” to be considered a “regular” prey item for two demersal fish species ( Notothenia rossii and N. coriiceps ) of Potter Cove, South Shetland Islands, using a multimarker (COI and 18S) metabarcoding approach. We found that GZP taxa commonly occurred in the diets of both species, represented by pelagic tunicates (appendicularians, salps), cnidarians, chaetognaths and ctenophores. Salps were the most abundant prey group, preyed upon by each individual of both species, reaching 98.7% relative read abundance for 18S. We recovered a wide range of different taxa in their diets, from primary producers to highly abundant invertebrates, thus the two nototheniid species can be regarded as “natural samplers” of the ecosystem in study. Finally, we want to point out the importance of multimarker metabarcoding approaches for broad ecological assessments, given the differential amplification and sequencing success of different markers for specific groups and the unequal taxonomic coverage of the reference databases. The output of each marker was highly complementary, since an important prey item such as salps, was only detected with 18S, while other taxa (e.g., Arthropoda) were represented with a higher taxonomic resolution with COI. Article in Journal/Newspaper Antarc* Antarctic Notothenia rossii South Shetland Islands Southern Ocean Wiley Online Library Antarctic Potter Cove South Shetland Islands Southern Ocean Environmental DNA 6 3 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Gelatinous zooplankton (GZP), i.e., ctenophores, cnidarian medusae, chaetognaths, appendicularians and salps, are considered climate change winners. This becomes particularly obvious in the Southern Ocean, which has undergone a significant shift from a krill‐based to a salp‐based ecosystem over the last decades. A better knowledge on the role of gelatinous invertebrates as prey is needed to predict the impact of such a gelatinous shift. Until recently, GZP was considered as a “trophic dead end”. However, their true importance in diets has remained unresolved due to the rapid digestion of their watery and soft tissues in predators' stomachs. In this study, we want to validate the paradigm shift from GZP being considered as “survival food” to be considered a “regular” prey item for two demersal fish species ( Notothenia rossii and N. coriiceps ) of Potter Cove, South Shetland Islands, using a multimarker (COI and 18S) metabarcoding approach. We found that GZP taxa commonly occurred in the diets of both species, represented by pelagic tunicates (appendicularians, salps), cnidarians, chaetognaths and ctenophores. Salps were the most abundant prey group, preyed upon by each individual of both species, reaching 98.7% relative read abundance for 18S. We recovered a wide range of different taxa in their diets, from primary producers to highly abundant invertebrates, thus the two nototheniid species can be regarded as “natural samplers” of the ecosystem in study. Finally, we want to point out the importance of multimarker metabarcoding approaches for broad ecological assessments, given the differential amplification and sequencing success of different markers for specific groups and the unequal taxonomic coverage of the reference databases. The output of each marker was highly complementary, since an important prey item such as salps, was only detected with 18S, while other taxa (e.g., Arthropoda) were represented with a higher taxonomic resolution with COI. |
author2 |
Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research Deutsche Forschungsgemeinschaft Fondo para la Investigación Científica y Tecnológica |
format |
Article in Journal/Newspaper |
author |
Ruiz, Micaela B. Moreira, Eugenia Novillo, Manuel Neuhaus, Stefan Leese, Florian Havermans, Charlotte |
spellingShingle |
Ruiz, Micaela B. Moreira, Eugenia Novillo, Manuel Neuhaus, Stefan Leese, Florian Havermans, Charlotte Detecting the invisible through DNA metabarcoding: The role of gelatinous taxa in the diet of two demersal Antarctic key stone fish species (Notothenioidei) |
author_facet |
Ruiz, Micaela B. Moreira, Eugenia Novillo, Manuel Neuhaus, Stefan Leese, Florian Havermans, Charlotte |
author_sort |
Ruiz, Micaela B. |
title |
Detecting the invisible through DNA metabarcoding: The role of gelatinous taxa in the diet of two demersal Antarctic key stone fish species (Notothenioidei) |
title_short |
Detecting the invisible through DNA metabarcoding: The role of gelatinous taxa in the diet of two demersal Antarctic key stone fish species (Notothenioidei) |
title_full |
Detecting the invisible through DNA metabarcoding: The role of gelatinous taxa in the diet of two demersal Antarctic key stone fish species (Notothenioidei) |
title_fullStr |
Detecting the invisible through DNA metabarcoding: The role of gelatinous taxa in the diet of two demersal Antarctic key stone fish species (Notothenioidei) |
title_full_unstemmed |
Detecting the invisible through DNA metabarcoding: The role of gelatinous taxa in the diet of two demersal Antarctic key stone fish species (Notothenioidei) |
title_sort |
detecting the invisible through dna metabarcoding: the role of gelatinous taxa in the diet of two demersal antarctic key stone fish species (notothenioidei) |
publisher |
Wiley |
publishDate |
2024 |
url |
http://dx.doi.org/10.1002/edn3.561 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.561 |
geographic |
Antarctic Potter Cove South Shetland Islands Southern Ocean |
geographic_facet |
Antarctic Potter Cove South Shetland Islands Southern Ocean |
genre |
Antarc* Antarctic Notothenia rossii South Shetland Islands Southern Ocean |
genre_facet |
Antarc* Antarctic Notothenia rossii South Shetland Islands Southern Ocean |
op_source |
Environmental DNA volume 6, issue 3 ISSN 2637-4943 2637-4943 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/edn3.561 |
container_title |
Environmental DNA |
container_volume |
6 |
container_issue |
3 |
_version_ |
1809819362339586048 |