Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge eDNA surveys
Abstract The Ross Sea, Antarctica, while largely pristine, is experiencing increased anthropogenic pressures, necessitating enhanced biomonitoring efforts for conservation purposes. Environmental DNA (eDNA) extracted from marine sponges provides a promising approach for biodiversity monitoring in re...
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Online Access: | http://dx.doi.org/10.1002/edn3.500 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.500 |
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crwiley:10.1002/edn3.500 2024-06-02T07:58:43+00:00 Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge eDNA surveys Jeunen, Gert‐Jan Lamare, Miles Cummings, Vonda Treece, Jackson Ferreira, Sara Massuger, Jack Pryor Rodgers, Lily Tait, Leigh Lust, Bobby Wilkinson, Shaun Mariani, Stefano Mills, Sadie Gemmell, Neil Marsden Fund Ministry of Business, Innovation and Employment 2023 http://dx.doi.org/10.1002/edn3.500 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.500 en eng Wiley http://creativecommons.org/licenses/by-nc/4.0/ Environmental DNA volume 5, issue 6, page 1780-1792 ISSN 2637-4943 2637-4943 journal-article 2023 crwiley https://doi.org/10.1002/edn3.500 2024-05-06T07:04:44Z Abstract The Ross Sea, Antarctica, while largely pristine, is experiencing increased anthropogenic pressures, necessitating enhanced biomonitoring efforts for conservation purposes. Environmental DNA (eDNA) extracted from marine sponges provides a promising approach for biodiversity monitoring in remote areas by circumventing the need for time‐consuming water filtration. Investigations into the efficacy of eDNA signal detection across the tree of life from marine sponges have yet to be fully explored. Here, we conducted a seawater and sponge eDNA metabarcoding survey at seven coastal locations in the Ross Sea to assess spatial eukaryote biodiversity patterns and investigate eDNA signal differences between both substrates. In total, we detected 1450 operational taxonomic units (OTUs) across 30 phyla. Significant differences in water and sponge eDNA signal richness and composition were observed, with a partial overlap in OTU detection between both substrates and, thereby, underscoring the crucial role of substrate selection in eDNA metabarcoding surveys. Furthermore, alpha and beta diversity analyses revealed distinct eDNA signals among sampling locations, which were corroborated by known species distributions. However, only 135 OTUs (9%) could be successfully assigned to species level, and 574 OTUs (40%) were unable to be taxonomically classified, due to limitations in the reference database. Our results provide evidence for the potential of eDNA monitoring in remote areas, demonstrate the need to consider more sophisticated sampling strategies whereby multiple eDNA substrates are incorporated, and highlight the importance of complete reference databases for robust taxonomy assignment of eDNA signals. Article in Journal/Newspaper Antarc* Antarctica Ross Sea Wiley Online Library Ross Sea Environmental DNA 5 6 1780 1792 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The Ross Sea, Antarctica, while largely pristine, is experiencing increased anthropogenic pressures, necessitating enhanced biomonitoring efforts for conservation purposes. Environmental DNA (eDNA) extracted from marine sponges provides a promising approach for biodiversity monitoring in remote areas by circumventing the need for time‐consuming water filtration. Investigations into the efficacy of eDNA signal detection across the tree of life from marine sponges have yet to be fully explored. Here, we conducted a seawater and sponge eDNA metabarcoding survey at seven coastal locations in the Ross Sea to assess spatial eukaryote biodiversity patterns and investigate eDNA signal differences between both substrates. In total, we detected 1450 operational taxonomic units (OTUs) across 30 phyla. Significant differences in water and sponge eDNA signal richness and composition were observed, with a partial overlap in OTU detection between both substrates and, thereby, underscoring the crucial role of substrate selection in eDNA metabarcoding surveys. Furthermore, alpha and beta diversity analyses revealed distinct eDNA signals among sampling locations, which were corroborated by known species distributions. However, only 135 OTUs (9%) could be successfully assigned to species level, and 574 OTUs (40%) were unable to be taxonomically classified, due to limitations in the reference database. Our results provide evidence for the potential of eDNA monitoring in remote areas, demonstrate the need to consider more sophisticated sampling strategies whereby multiple eDNA substrates are incorporated, and highlight the importance of complete reference databases for robust taxonomy assignment of eDNA signals. |
author2 |
Marsden Fund Ministry of Business, Innovation and Employment |
format |
Article in Journal/Newspaper |
author |
Jeunen, Gert‐Jan Lamare, Miles Cummings, Vonda Treece, Jackson Ferreira, Sara Massuger, Jack Pryor Rodgers, Lily Tait, Leigh Lust, Bobby Wilkinson, Shaun Mariani, Stefano Mills, Sadie Gemmell, Neil |
spellingShingle |
Jeunen, Gert‐Jan Lamare, Miles Cummings, Vonda Treece, Jackson Ferreira, Sara Massuger, Jack Pryor Rodgers, Lily Tait, Leigh Lust, Bobby Wilkinson, Shaun Mariani, Stefano Mills, Sadie Gemmell, Neil Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge eDNA surveys |
author_facet |
Jeunen, Gert‐Jan Lamare, Miles Cummings, Vonda Treece, Jackson Ferreira, Sara Massuger, Jack Pryor Rodgers, Lily Tait, Leigh Lust, Bobby Wilkinson, Shaun Mariani, Stefano Mills, Sadie Gemmell, Neil |
author_sort |
Jeunen, Gert‐Jan |
title |
Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge eDNA surveys |
title_short |
Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge eDNA surveys |
title_full |
Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge eDNA surveys |
title_fullStr |
Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge eDNA surveys |
title_full_unstemmed |
Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge eDNA surveys |
title_sort |
unveiling the hidden diversity of marine eukaryotes in the ross sea: a comparative analysis of seawater and sponge edna surveys |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1002/edn3.500 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.500 |
geographic |
Ross Sea |
geographic_facet |
Ross Sea |
genre |
Antarc* Antarctica Ross Sea |
genre_facet |
Antarc* Antarctica Ross Sea |
op_source |
Environmental DNA volume 5, issue 6, page 1780-1792 ISSN 2637-4943 2637-4943 |
op_rights |
http://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.1002/edn3.500 |
container_title |
Environmental DNA |
container_volume |
5 |
container_issue |
6 |
container_start_page |
1780 |
op_container_end_page |
1792 |
_version_ |
1800742216577056768 |