Monitoring presence and abundance of two gyrodactylid ectoparasites and their salmonid hosts using environmental DNA
Abstract Background Invasive species represent a major challenge for the conservation of biodiversity. The invasive ectoparasitic fluke Gyrodactylus salaris is considered one of the major threats to the Atlantic salmon ( Salmo salar ), and the parasite has so far been detected in 50 rivers in Norway...
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crwiley:10.1002/edn3.45 2024-09-09T19:30:38+00:00 Monitoring presence and abundance of two gyrodactylid ectoparasites and their salmonid hosts using environmental DNA Fossøy, Frode Brandsegg, Hege Sivertsgård, Rolf Pettersen, Oskar Sandercock, Brett K. Solem, Øyvind Hindar, Kjetil Mo, Tor Atle Miljødirektoratet 2019 http://dx.doi.org/10.1002/edn3.45 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fedn3.45 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.45 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/edn3.45 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Environmental DNA volume 2, issue 1, page 53-62 ISSN 2637-4943 2637-4943 journal-article 2019 crwiley https://doi.org/10.1002/edn3.45 2024-08-13T04:18:33Z Abstract Background Invasive species represent a major challenge for the conservation of biodiversity. The invasive ectoparasitic fluke Gyrodactylus salaris is considered one of the major threats to the Atlantic salmon ( Salmo salar ), and the parasite has so far been detected in 50 rivers in Norway. Aims We investigate environmental DNA (eDNA) as a tool for detecting and assessing relative abundance of G. salaris and Atlantic salmon, upstream and downstream of a recently constructed artificial migration barrier in the River Driva in Norway. In addition, we also use eDNA to assess abundance of the less pathogenic G. derjavinoides and its main host, the brown trout ( S. trutta ). Material & Methods We filtered 1 L and 10 L of water through a 0.45 μm cellulose filter and a 2.0 μm glass fiber filter, respectively, at nine different localities along the river. Concentrations of eDNA were assessed using droplet digital PCR (ddPCR) and compared to parasite abundance based on conventional methodology using electrofishing and the counting of individual parasites on juvenile salmon. Results All four species could successfully be detected from water samples using two different protocols varying in sample volumes, filter types, and DNA‐isolation methods. However, eDNA‐occupancy modeling revealed that the probability of detecting the two gyrodactylid species was higher when filtering 10 L water through a 2.0 μm glass fiber filter ( p > .99) than when filtering 1 L water through a 0.45 μm cellulose filter ( p = .48–.78). The eDNA concentrations of the two fish species were markedly higher below the migration barrier, reflecting the expected higher biomass of fish. For the two gyrodactylid parasites, eDNA concentrations showed a peak upstream of the migration barrier and decreased below the migration barrier. The observed pattern was consistent with parasite abundance based on conventional methodology. Discussion Assessing abundance in rivers using eDNA is challenging and potentially influenced by downstream ... Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Driva ENVELOPE(9.633,9.633,62.533,62.533) Norway Environmental DNA 2 1 53 62 |
institution |
Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Background Invasive species represent a major challenge for the conservation of biodiversity. The invasive ectoparasitic fluke Gyrodactylus salaris is considered one of the major threats to the Atlantic salmon ( Salmo salar ), and the parasite has so far been detected in 50 rivers in Norway. Aims We investigate environmental DNA (eDNA) as a tool for detecting and assessing relative abundance of G. salaris and Atlantic salmon, upstream and downstream of a recently constructed artificial migration barrier in the River Driva in Norway. In addition, we also use eDNA to assess abundance of the less pathogenic G. derjavinoides and its main host, the brown trout ( S. trutta ). Material & Methods We filtered 1 L and 10 L of water through a 0.45 μm cellulose filter and a 2.0 μm glass fiber filter, respectively, at nine different localities along the river. Concentrations of eDNA were assessed using droplet digital PCR (ddPCR) and compared to parasite abundance based on conventional methodology using electrofishing and the counting of individual parasites on juvenile salmon. Results All four species could successfully be detected from water samples using two different protocols varying in sample volumes, filter types, and DNA‐isolation methods. However, eDNA‐occupancy modeling revealed that the probability of detecting the two gyrodactylid species was higher when filtering 10 L water through a 2.0 μm glass fiber filter ( p > .99) than when filtering 1 L water through a 0.45 μm cellulose filter ( p = .48–.78). The eDNA concentrations of the two fish species were markedly higher below the migration barrier, reflecting the expected higher biomass of fish. For the two gyrodactylid parasites, eDNA concentrations showed a peak upstream of the migration barrier and decreased below the migration barrier. The observed pattern was consistent with parasite abundance based on conventional methodology. Discussion Assessing abundance in rivers using eDNA is challenging and potentially influenced by downstream ... |
author2 |
Miljødirektoratet |
format |
Article in Journal/Newspaper |
author |
Fossøy, Frode Brandsegg, Hege Sivertsgård, Rolf Pettersen, Oskar Sandercock, Brett K. Solem, Øyvind Hindar, Kjetil Mo, Tor Atle |
spellingShingle |
Fossøy, Frode Brandsegg, Hege Sivertsgård, Rolf Pettersen, Oskar Sandercock, Brett K. Solem, Øyvind Hindar, Kjetil Mo, Tor Atle Monitoring presence and abundance of two gyrodactylid ectoparasites and their salmonid hosts using environmental DNA |
author_facet |
Fossøy, Frode Brandsegg, Hege Sivertsgård, Rolf Pettersen, Oskar Sandercock, Brett K. Solem, Øyvind Hindar, Kjetil Mo, Tor Atle |
author_sort |
Fossøy, Frode |
title |
Monitoring presence and abundance of two gyrodactylid ectoparasites and their salmonid hosts using environmental DNA |
title_short |
Monitoring presence and abundance of two gyrodactylid ectoparasites and their salmonid hosts using environmental DNA |
title_full |
Monitoring presence and abundance of two gyrodactylid ectoparasites and their salmonid hosts using environmental DNA |
title_fullStr |
Monitoring presence and abundance of two gyrodactylid ectoparasites and their salmonid hosts using environmental DNA |
title_full_unstemmed |
Monitoring presence and abundance of two gyrodactylid ectoparasites and their salmonid hosts using environmental DNA |
title_sort |
monitoring presence and abundance of two gyrodactylid ectoparasites and their salmonid hosts using environmental dna |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1002/edn3.45 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fedn3.45 https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.45 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/edn3.45 |
long_lat |
ENVELOPE(9.633,9.633,62.533,62.533) |
geographic |
Driva Norway |
geographic_facet |
Driva Norway |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Environmental DNA volume 2, issue 1, page 53-62 ISSN 2637-4943 2637-4943 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/edn3.45 |
container_title |
Environmental DNA |
container_volume |
2 |
container_issue |
1 |
container_start_page |
53 |
op_container_end_page |
62 |
_version_ |
1809899626896031744 |