Evaluating the sampling effort for the metabarcoding‐based detection of fish environmental DNA in the open ocean
Abstract Clarifying the effect of the sampling protocol on the detection of environmental DNA (eDNA) is essential for appropriately designing biodiversity research. However, technical issues influencing eDNA detection in the open ocean, which consists of water masses with varying environmental condi...
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Online Access: | http://dx.doi.org/10.1002/ece3.9921 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.9921 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ece3.9921 |
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crwiley:10.1002/ece3.9921 2024-03-24T09:00:12+00:00 Evaluating the sampling effort for the metabarcoding‐based detection of fish environmental DNA in the open ocean Kawakami, Tatsuya Yamazaki, Aya Asami, Maki Goto, Yuko Yamanaka, Hiroki Hyodo, Susumu Ueno, Hiromichi Kasai, Akihide Japan Society for the Promotion of Science Ministry of Education, Culture, Sports, Science and Technology University of Tokyo 2023 http://dx.doi.org/10.1002/ece3.9921 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.9921 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ece3.9921 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Ecology and Evolution volume 13, issue 3 ISSN 2045-7758 2045-7758 Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics journal-article 2023 crwiley https://doi.org/10.1002/ece3.9921 2024-02-28T02:15:39Z Abstract Clarifying the effect of the sampling protocol on the detection of environmental DNA (eDNA) is essential for appropriately designing biodiversity research. However, technical issues influencing eDNA detection in the open ocean, which consists of water masses with varying environmental conditions, have not been thoroughly investigated. This study evaluated the sampling effort for the metabarcoding‐based detection of fish eDNA using replicate sampling with filters of different pore sizes (0.22 and 0.45 μm) in the subtropical and subarctic northwestern Pacific Ocean and Arctic Chukchi Sea. The asymptotic analysis predicted that the accumulation curves for detected taxa did not saturate in most cases, indicating that our sampling effort (7 or 8 replicates, corresponding to 10.5–40 L of filtration in total) was insufficient to fully assess the species diversity in the open ocean and that tens of replicates or a substantial filtration volume were required. The Jaccard dissimilarities between filtration replicates were comparable with those between the filter types at any site. In subtropical and subarctic sites, turnover dominated the dissimilarity, suggesting that the filter pore size had a negligible effect. In contrast, nestedness dominated the dissimilarity in the Chukchi Sea, implying that the 0.22 μm filter could collect a broader range of eDNA than the 0.45 μm filter. Therefore, the effect of filter selection on the collection of fish eDNA likely varies depending on the region. These findings highlight the highly stochastic nature of fish eDNA collection in the open ocean and the difficulty of standardizing the sampling protocol across various water masses. Article in Journal/Newspaper Arctic Chukchi Chukchi Sea Subarctic Wiley Online Library Arctic Chukchi Sea Pacific Ecology and Evolution 13 3 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics Kawakami, Tatsuya Yamazaki, Aya Asami, Maki Goto, Yuko Yamanaka, Hiroki Hyodo, Susumu Ueno, Hiromichi Kasai, Akihide Evaluating the sampling effort for the metabarcoding‐based detection of fish environmental DNA in the open ocean |
topic_facet |
Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics |
description |
Abstract Clarifying the effect of the sampling protocol on the detection of environmental DNA (eDNA) is essential for appropriately designing biodiversity research. However, technical issues influencing eDNA detection in the open ocean, which consists of water masses with varying environmental conditions, have not been thoroughly investigated. This study evaluated the sampling effort for the metabarcoding‐based detection of fish eDNA using replicate sampling with filters of different pore sizes (0.22 and 0.45 μm) in the subtropical and subarctic northwestern Pacific Ocean and Arctic Chukchi Sea. The asymptotic analysis predicted that the accumulation curves for detected taxa did not saturate in most cases, indicating that our sampling effort (7 or 8 replicates, corresponding to 10.5–40 L of filtration in total) was insufficient to fully assess the species diversity in the open ocean and that tens of replicates or a substantial filtration volume were required. The Jaccard dissimilarities between filtration replicates were comparable with those between the filter types at any site. In subtropical and subarctic sites, turnover dominated the dissimilarity, suggesting that the filter pore size had a negligible effect. In contrast, nestedness dominated the dissimilarity in the Chukchi Sea, implying that the 0.22 μm filter could collect a broader range of eDNA than the 0.45 μm filter. Therefore, the effect of filter selection on the collection of fish eDNA likely varies depending on the region. These findings highlight the highly stochastic nature of fish eDNA collection in the open ocean and the difficulty of standardizing the sampling protocol across various water masses. |
author2 |
Japan Society for the Promotion of Science Ministry of Education, Culture, Sports, Science and Technology University of Tokyo |
format |
Article in Journal/Newspaper |
author |
Kawakami, Tatsuya Yamazaki, Aya Asami, Maki Goto, Yuko Yamanaka, Hiroki Hyodo, Susumu Ueno, Hiromichi Kasai, Akihide |
author_facet |
Kawakami, Tatsuya Yamazaki, Aya Asami, Maki Goto, Yuko Yamanaka, Hiroki Hyodo, Susumu Ueno, Hiromichi Kasai, Akihide |
author_sort |
Kawakami, Tatsuya |
title |
Evaluating the sampling effort for the metabarcoding‐based detection of fish environmental DNA in the open ocean |
title_short |
Evaluating the sampling effort for the metabarcoding‐based detection of fish environmental DNA in the open ocean |
title_full |
Evaluating the sampling effort for the metabarcoding‐based detection of fish environmental DNA in the open ocean |
title_fullStr |
Evaluating the sampling effort for the metabarcoding‐based detection of fish environmental DNA in the open ocean |
title_full_unstemmed |
Evaluating the sampling effort for the metabarcoding‐based detection of fish environmental DNA in the open ocean |
title_sort |
evaluating the sampling effort for the metabarcoding‐based detection of fish environmental dna in the open ocean |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1002/ece3.9921 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.9921 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ece3.9921 |
geographic |
Arctic Chukchi Sea Pacific |
geographic_facet |
Arctic Chukchi Sea Pacific |
genre |
Arctic Chukchi Chukchi Sea Subarctic |
genre_facet |
Arctic Chukchi Chukchi Sea Subarctic |
op_source |
Ecology and Evolution volume 13, issue 3 ISSN 2045-7758 2045-7758 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/ece3.9921 |
container_title |
Ecology and Evolution |
container_volume |
13 |
container_issue |
3 |
_version_ |
1794400033787871232 |