Estimating number of European eel ( Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers
Abstract Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approach...
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crwiley:10.1002/ece3.9785 2024-06-02T07:55:09+00:00 Estimating number of European eel ( Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers Halvorsen, Silje Korslund, Lars Mattingsdal, Morten Slettan, Audun 2023 http://dx.doi.org/10.1002/ece3.9785 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.9785 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ece3.9785 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Ecology and Evolution volume 13, issue 2 ISSN 2045-7758 2045-7758 journal-article 2023 crwiley https://doi.org/10.1002/ece3.9785 2024-05-03T10:42:39Z Abstract Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer a way to sample genetic material noninvasively. In attempts to estimate population size of aquatic species using eDNA, researchers have found positive correlations between biomass and eDNA concentrations, but the approach is debated because of variations in the production and degrading of DNA in water. Recently, a more accurate eDNA‐approach has emerged, focusing on the genomic differences between individuals. In this study, we used eDNA from water samples to estimate the number of European eel ( Anguilla anguilla ) individuals by examining haplotypes in the mitochondrial D‐loop region, both in a closed aquatic environment with 10 eels of known haplotypes and in three rivers. The results revealed that it was possible to find every eel haplotype in the eDNA sample collected from the closed environment. We also found 13 unique haplotypes in the eDNA samples from the three rivers, which probably represent 13 eel individuals. This means that it is possible to obtain genomic information from European eel eDNA in water; however, more research is needed to develop the approach into a possible future tool for population quantification. Article in Journal/Newspaper Anguilla anguilla European eel Wiley Online Library Ecology and Evolution 13 2 |
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Abstract Knowledge about population genetic data is important for effective conservation management. Genetic research traditionally requires sampling directly from the organism, for example tissue, which can be challenging, time‐consuming, and harmful to the animal. Environmental DNA (eDNA) approaches offer a way to sample genetic material noninvasively. In attempts to estimate population size of aquatic species using eDNA, researchers have found positive correlations between biomass and eDNA concentrations, but the approach is debated because of variations in the production and degrading of DNA in water. Recently, a more accurate eDNA‐approach has emerged, focusing on the genomic differences between individuals. In this study, we used eDNA from water samples to estimate the number of European eel ( Anguilla anguilla ) individuals by examining haplotypes in the mitochondrial D‐loop region, both in a closed aquatic environment with 10 eels of known haplotypes and in three rivers. The results revealed that it was possible to find every eel haplotype in the eDNA sample collected from the closed environment. We also found 13 unique haplotypes in the eDNA samples from the three rivers, which probably represent 13 eel individuals. This means that it is possible to obtain genomic information from European eel eDNA in water; however, more research is needed to develop the approach into a possible future tool for population quantification. |
format |
Article in Journal/Newspaper |
author |
Halvorsen, Silje Korslund, Lars Mattingsdal, Morten Slettan, Audun |
spellingShingle |
Halvorsen, Silje Korslund, Lars Mattingsdal, Morten Slettan, Audun Estimating number of European eel ( Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
author_facet |
Halvorsen, Silje Korslund, Lars Mattingsdal, Morten Slettan, Audun |
author_sort |
Halvorsen, Silje |
title |
Estimating number of European eel ( Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_short |
Estimating number of European eel ( Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_full |
Estimating number of European eel ( Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_fullStr |
Estimating number of European eel ( Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_full_unstemmed |
Estimating number of European eel ( Anguilla anguilla) individuals using environmental DNA and haplotype count in small rivers |
title_sort |
estimating number of european eel ( anguilla anguilla) individuals using environmental dna and haplotype count in small rivers |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1002/ece3.9785 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.9785 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ece3.9785 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Ecology and Evolution volume 13, issue 2 ISSN 2045-7758 2045-7758 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/ece3.9785 |
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Ecology and Evolution |
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13 |
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2 |
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1800746268747628544 |