Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas
Abstract Understanding the environmental responses of an invasive species is critical in predicting how ecosystem composition may be transformed in the future, especially under climate change. In this study, C rassostrea gigas , a species well adapted to the highly variable intertidal environment, w...
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crwiley:10.1002/ece3.719 2024-06-23T07:52:17+00:00 Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas Clark, Melody S. Thorne, Michael A. S. Amaral, Ana Vieira, Florbela Batista, Frederico M. Reis, João Power, Deborah M. 2013 http://dx.doi.org/10.1002/ece3.719 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.719 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.719 en eng Wiley http://creativecommons.org/licenses/by/3.0/ Ecology and Evolution volume 3, issue 10, page 3283-3297 ISSN 2045-7758 2045-7758 journal-article 2013 crwiley https://doi.org/10.1002/ece3.719 2024-06-13T04:25:15Z Abstract Understanding the environmental responses of an invasive species is critical in predicting how ecosystem composition may be transformed in the future, especially under climate change. In this study, C rassostrea gigas , a species well adapted to the highly variable intertidal environment, was exposed to the chronic environmental challenges of temperature (19 and 24°C) and pH (ambient seawater and a reduction of 0.4 pH units) in an extended 3‐month laboratory‐based study. Physiological parameters were measured (condition index, shell growth, respiration, excretion rates, O:N ratios, and ability to repair shell damage) alongside molecular analyses. Temperature was by far the most important stressor, as demonstrated by reduced condition indexes and shell growth at 24°C, with relatively little effect detected for pH . Transcriptional profiling using candidate genes and SOLiD sequencing of mantle tissue revealed that classical “stress” genes, previously reported to be upregulated under acute temperature challenges, were not significantly expressed in any of the treatments, emphasizing the different response between acute and longer term chronic stress. The transcriptional profiling also elaborated on the cellular responses underpinning the physiological results, including the identification of the PI 3K/ AKT / mTOR pathway as a potentially novel marker for chronic environmental challenge. This study represents a first attempt to understand the energetic consequences of cumulative thermal stress on the intertidal C . gigas which could significantly impact on coastal ecosystem biodiversity and function in the future. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific Ecology and Evolution n/a n/a |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Understanding the environmental responses of an invasive species is critical in predicting how ecosystem composition may be transformed in the future, especially under climate change. In this study, C rassostrea gigas , a species well adapted to the highly variable intertidal environment, was exposed to the chronic environmental challenges of temperature (19 and 24°C) and pH (ambient seawater and a reduction of 0.4 pH units) in an extended 3‐month laboratory‐based study. Physiological parameters were measured (condition index, shell growth, respiration, excretion rates, O:N ratios, and ability to repair shell damage) alongside molecular analyses. Temperature was by far the most important stressor, as demonstrated by reduced condition indexes and shell growth at 24°C, with relatively little effect detected for pH . Transcriptional profiling using candidate genes and SOLiD sequencing of mantle tissue revealed that classical “stress” genes, previously reported to be upregulated under acute temperature challenges, were not significantly expressed in any of the treatments, emphasizing the different response between acute and longer term chronic stress. The transcriptional profiling also elaborated on the cellular responses underpinning the physiological results, including the identification of the PI 3K/ AKT / mTOR pathway as a potentially novel marker for chronic environmental challenge. This study represents a first attempt to understand the energetic consequences of cumulative thermal stress on the intertidal C . gigas which could significantly impact on coastal ecosystem biodiversity and function in the future. |
format |
Article in Journal/Newspaper |
author |
Clark, Melody S. Thorne, Michael A. S. Amaral, Ana Vieira, Florbela Batista, Frederico M. Reis, João Power, Deborah M. |
spellingShingle |
Clark, Melody S. Thorne, Michael A. S. Amaral, Ana Vieira, Florbela Batista, Frederico M. Reis, João Power, Deborah M. Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas |
author_facet |
Clark, Melody S. Thorne, Michael A. S. Amaral, Ana Vieira, Florbela Batista, Frederico M. Reis, João Power, Deborah M. |
author_sort |
Clark, Melody S. |
title |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas |
title_short |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas |
title_full |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas |
title_fullStr |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas |
title_sort |
identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the pacific oyster, crassostrea gigas |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1002/ece3.719 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.719 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.719 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Ecology and Evolution volume 3, issue 10, page 3283-3297 ISSN 2045-7758 2045-7758 |
op_rights |
http://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.1002/ece3.719 |
container_title |
Ecology and Evolution |
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n/a |
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_version_ |
1802643556565778432 |