Genetic differentiation and asymmetric gene flow among Carpathian brown bear ( Ursus arctos) populations—Implications for conservation of transboundary populations
Abstract The abundance and distribution of large carnivores in Europe have been historically reduced. Their recovery requires multilevel coordination, especially regarding transboundary populations. Here, we apply nuclear and mitochondrial genetic markers to test for admixture level and its impact o...
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Online Access: | http://dx.doi.org/10.1002/ece3.4872 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.4872 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.4872 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ece3.4872 |
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crwiley:10.1002/ece3.4872 2024-06-02T08:15:36+00:00 Genetic differentiation and asymmetric gene flow among Carpathian brown bear ( Ursus arctos) populations—Implications for conservation of transboundary populations Matosiuk, Maciej Śmietana, Wojciech Czajkowska, Magdalena Paule, Ladislav Štofik, Jozef Krajmerová, Diana Bashta, Andriy‐Taras Jakimiuk, Stefan Ratkiewicz, Mirosław WWF Poland; EEA grants and Norway grants University of Bialystok 2019 http://dx.doi.org/10.1002/ece3.4872 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.4872 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.4872 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ece3.4872 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Ecology and Evolution volume 9, issue 3, page 1501-1511 ISSN 2045-7758 2045-7758 journal-article 2019 crwiley https://doi.org/10.1002/ece3.4872 2024-05-03T11:56:09Z Abstract The abundance and distribution of large carnivores in Europe have been historically reduced. Their recovery requires multilevel coordination, especially regarding transboundary populations. Here, we apply nuclear and mitochondrial genetic markers to test for admixture level and its impact on population genetic structure of contemporary brown bears ( Ursus arctos ) from the Eastern, Southern, and Western Carpathians. Carpathian Mountains (Europe). Nearly 400 noninvasive brown bear DNA samples from the Western (Poland) and Eastern Carpathians (Bieszczady Mountains in Poland, Slovakia, Ukraine) were collected. Together with DNA isolates from Slovakia and Romania, they were analyzed using the set of eight microsatellite loci and two mtDNA regions (control region and cytochrome b ). A set of 113 individuals with complete genotypes was used to investigate genetic differentiation across national boundaries, genetic structuring within and between populations, and movement between populations. Transboundary brown bear subpopulations (Slovakia and Poland) did not show significant internal genetic structure, and thus were treated as cohesive units. All brown bears from the Western Carpathians carried mitochondrial haplotypes from the Eastern lineage, while the Western lineage prevailed in the brown bears from the Bieszczady Mountains. Despite similar levels of microsatellite variability, we documented significant differentiation among the studied populations for nuclear markers and mtDNA. We also detected male‐biased and asymmetrical movement into the Bieszczady Mountains population from the Western Carpathians. Our findings suggest initial colonization of the Western Carpathians by brown bears possessing mtDNA from the Eastern lineage. Genetic structuring among populations at microsatellite loci could be a result of human‐mediated alterations. Detected asymmetric gene flow suggests ongoing expansion from more abundant populations into the Bieszczady Mountains and thus supports a metapopulation model. The ... Article in Journal/Newspaper Ursus arctos Wiley Online Library Ecology and Evolution 9 3 1501 1511 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The abundance and distribution of large carnivores in Europe have been historically reduced. Their recovery requires multilevel coordination, especially regarding transboundary populations. Here, we apply nuclear and mitochondrial genetic markers to test for admixture level and its impact on population genetic structure of contemporary brown bears ( Ursus arctos ) from the Eastern, Southern, and Western Carpathians. Carpathian Mountains (Europe). Nearly 400 noninvasive brown bear DNA samples from the Western (Poland) and Eastern Carpathians (Bieszczady Mountains in Poland, Slovakia, Ukraine) were collected. Together with DNA isolates from Slovakia and Romania, they were analyzed using the set of eight microsatellite loci and two mtDNA regions (control region and cytochrome b ). A set of 113 individuals with complete genotypes was used to investigate genetic differentiation across national boundaries, genetic structuring within and between populations, and movement between populations. Transboundary brown bear subpopulations (Slovakia and Poland) did not show significant internal genetic structure, and thus were treated as cohesive units. All brown bears from the Western Carpathians carried mitochondrial haplotypes from the Eastern lineage, while the Western lineage prevailed in the brown bears from the Bieszczady Mountains. Despite similar levels of microsatellite variability, we documented significant differentiation among the studied populations for nuclear markers and mtDNA. We also detected male‐biased and asymmetrical movement into the Bieszczady Mountains population from the Western Carpathians. Our findings suggest initial colonization of the Western Carpathians by brown bears possessing mtDNA from the Eastern lineage. Genetic structuring among populations at microsatellite loci could be a result of human‐mediated alterations. Detected asymmetric gene flow suggests ongoing expansion from more abundant populations into the Bieszczady Mountains and thus supports a metapopulation model. The ... |
author2 |
WWF Poland; EEA grants and Norway grants University of Bialystok |
format |
Article in Journal/Newspaper |
author |
Matosiuk, Maciej Śmietana, Wojciech Czajkowska, Magdalena Paule, Ladislav Štofik, Jozef Krajmerová, Diana Bashta, Andriy‐Taras Jakimiuk, Stefan Ratkiewicz, Mirosław |
spellingShingle |
Matosiuk, Maciej Śmietana, Wojciech Czajkowska, Magdalena Paule, Ladislav Štofik, Jozef Krajmerová, Diana Bashta, Andriy‐Taras Jakimiuk, Stefan Ratkiewicz, Mirosław Genetic differentiation and asymmetric gene flow among Carpathian brown bear ( Ursus arctos) populations—Implications for conservation of transboundary populations |
author_facet |
Matosiuk, Maciej Śmietana, Wojciech Czajkowska, Magdalena Paule, Ladislav Štofik, Jozef Krajmerová, Diana Bashta, Andriy‐Taras Jakimiuk, Stefan Ratkiewicz, Mirosław |
author_sort |
Matosiuk, Maciej |
title |
Genetic differentiation and asymmetric gene flow among Carpathian brown bear ( Ursus arctos) populations—Implications for conservation of transboundary populations |
title_short |
Genetic differentiation and asymmetric gene flow among Carpathian brown bear ( Ursus arctos) populations—Implications for conservation of transboundary populations |
title_full |
Genetic differentiation and asymmetric gene flow among Carpathian brown bear ( Ursus arctos) populations—Implications for conservation of transboundary populations |
title_fullStr |
Genetic differentiation and asymmetric gene flow among Carpathian brown bear ( Ursus arctos) populations—Implications for conservation of transboundary populations |
title_full_unstemmed |
Genetic differentiation and asymmetric gene flow among Carpathian brown bear ( Ursus arctos) populations—Implications for conservation of transboundary populations |
title_sort |
genetic differentiation and asymmetric gene flow among carpathian brown bear ( ursus arctos) populations—implications for conservation of transboundary populations |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1002/ece3.4872 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.4872 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.4872 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ece3.4872 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Ecology and Evolution volume 9, issue 3, page 1501-1511 ISSN 2045-7758 2045-7758 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/ece3.4872 |
container_title |
Ecology and Evolution |
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9 |
container_issue |
3 |
container_start_page |
1501 |
op_container_end_page |
1511 |
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1800739829041856512 |