No support for cryptic choice by ovarian fluid in an external fertilizer
Abstract Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior—the Arctic charr ( Salvelinus alpinus ). We physically separa...
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Online Access: | http://dx.doi.org/10.1002/ece3.4628 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.4628 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.4628 |
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crwiley:10.1002/ece3.4628 2024-06-02T08:00:06+00:00 No support for cryptic choice by ovarian fluid in an external fertilizer Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar Nansenfondet 2018 http://dx.doi.org/10.1002/ece3.4628 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.4628 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.4628 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Ecology and Evolution volume 8, issue 23, page 11763-11774 ISSN 2045-7758 2045-7758 journal-article 2018 crwiley https://doi.org/10.1002/ece3.4628 2024-05-03T10:53:22Z Abstract Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior—the Arctic charr ( Salvelinus alpinus ). We physically separated the OF from the eggs of reproductively active females and reintroduced either their own OF or fluid from another female to the eggs. The eggs were then fertilized in vitro in a replicated split‐brood design with sperm from two males under synchronized sperm competition trials, while also measuring sperm velocity of the individual males in the individual OFs. We found large effects of males, but no effect of females (i.e., eggs) on paternity, determined from microsatellites. More important, we found no effect of OF treatments on the relative paternity of the two competing males in each pair. This experimental setup does not provide support for the hypothesis that OF plays an important role as medium for cryptic female choice in charr. Power analyses revealed that our sample size is large enough to detect medium‐sized changes in relative paternity (medium‐sized effect sizes), but not large enough to detect small changes in relative paternity. More studies are needed before a conclusion can be drawn about OF's potential influence on paternity under sperm competition—even in charr. Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus Wiley Online Library Arctic Ecology and Evolution 8 23 11763 11774 |
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Wiley Online Library |
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English |
description |
Abstract Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior—the Arctic charr ( Salvelinus alpinus ). We physically separated the OF from the eggs of reproductively active females and reintroduced either their own OF or fluid from another female to the eggs. The eggs were then fertilized in vitro in a replicated split‐brood design with sperm from two males under synchronized sperm competition trials, while also measuring sperm velocity of the individual males in the individual OFs. We found large effects of males, but no effect of females (i.e., eggs) on paternity, determined from microsatellites. More important, we found no effect of OF treatments on the relative paternity of the two competing males in each pair. This experimental setup does not provide support for the hypothesis that OF plays an important role as medium for cryptic female choice in charr. Power analyses revealed that our sample size is large enough to detect medium‐sized changes in relative paternity (medium‐sized effect sizes), but not large enough to detect small changes in relative paternity. More studies are needed before a conclusion can be drawn about OF's potential influence on paternity under sperm competition—even in charr. |
author2 |
Nansenfondet |
format |
Article in Journal/Newspaper |
author |
Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar |
spellingShingle |
Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar No support for cryptic choice by ovarian fluid in an external fertilizer |
author_facet |
Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar |
author_sort |
Kleppe, Snøfrid April |
title |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_short |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_full |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_fullStr |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_full_unstemmed |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_sort |
no support for cryptic choice by ovarian fluid in an external fertilizer |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/ece3.4628 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.4628 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.4628 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
Ecology and Evolution volume 8, issue 23, page 11763-11774 ISSN 2045-7758 2045-7758 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/ece3.4628 |
container_title |
Ecology and Evolution |
container_volume |
8 |
container_issue |
23 |
container_start_page |
11763 |
op_container_end_page |
11774 |
_version_ |
1800744116700577792 |