Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden
Abstract Natural reservoirs of zoonotic pathogens generally seem to be capable of tolerating infections. Tolerance and its underlying mechanisms remain difficult to assess using experiments or wildlife surveys. High‐throughput sequencing technologies give the opportunity to investigate the genetic b...
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crwiley:10.1002/ece3.4603 2024-09-15T18:26:12+00:00 Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden Rohfritsch, Audrey Galan, Maxime Gautier, Mathieu Gharbi, Karim Olsson, Gert Gschloessl, Bernhard Zeimes, Caroline VanWambeke, Sophie Vitalis, Renaud Charbonnel, Nathalie 2018 http://dx.doi.org/10.1002/ece3.4603 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.4603 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.4603 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Ecology and Evolution volume 8, issue 22, page 11273-11292 ISSN 2045-7758 2045-7758 journal-article 2018 crwiley https://doi.org/10.1002/ece3.4603 2024-08-06T04:16:00Z Abstract Natural reservoirs of zoonotic pathogens generally seem to be capable of tolerating infections. Tolerance and its underlying mechanisms remain difficult to assess using experiments or wildlife surveys. High‐throughput sequencing technologies give the opportunity to investigate the genetic bases of tolerance, and the variability of its mechanisms in natural populations. In particular, population genomics may provide preliminary insights into the genes shaping tolerance and potentially influencing epidemiological dynamics. Here, we addressed these questions in the bank vole Myodes glareolus , the specific asymptomatic reservoir host of Puumala hantavirus (PUUV), which causes nephropathia epidemica (NE) in humans. Despite the continuous spatial distribution of M. glareolus in Sweden, NE is endemic to the northern part of the country. Northern bank vole populations in Sweden might exhibit tolerance strategies as a result of coadaptation with PUUV. This may favor the circulation and maintenance of PUUV and lead to high spatial risk of NE in northern Sweden. We performed a genome‐scan study to detect signatures of selection potentially correlated with spatial variations in tolerance to PUUV. We analyzed six bank vole populations from Sweden, sampled from northern NE‐endemic to southern NE‐free areas. We combined candidate gene analyses ( Tlr4 , Tlr7 , and Mx2 genes) and high‐throughput sequencing of restriction site‐associated DNA (RAD) markers. Outlier loci showed high levels of genetic differentiation and significant associations with environmental data including variations in the regional number of NE human cases. Among the 108 outliers that matched to mouse protein‐coding genes, 14 corresponded to immune‐related genes. The main biological pathways found to be significantly enriched corresponded to immune processes and responses to hantavirus, including the regulation of cytokine productions, TLR cascades, and IL‐7, VEGF, and JAK–STAT signaling. In the future, genome‐scan replicates and functional ... Article in Journal/Newspaper Northern Sweden Wiley Online Library Ecology and Evolution 8 22 11273 11292 |
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English |
description |
Abstract Natural reservoirs of zoonotic pathogens generally seem to be capable of tolerating infections. Tolerance and its underlying mechanisms remain difficult to assess using experiments or wildlife surveys. High‐throughput sequencing technologies give the opportunity to investigate the genetic bases of tolerance, and the variability of its mechanisms in natural populations. In particular, population genomics may provide preliminary insights into the genes shaping tolerance and potentially influencing epidemiological dynamics. Here, we addressed these questions in the bank vole Myodes glareolus , the specific asymptomatic reservoir host of Puumala hantavirus (PUUV), which causes nephropathia epidemica (NE) in humans. Despite the continuous spatial distribution of M. glareolus in Sweden, NE is endemic to the northern part of the country. Northern bank vole populations in Sweden might exhibit tolerance strategies as a result of coadaptation with PUUV. This may favor the circulation and maintenance of PUUV and lead to high spatial risk of NE in northern Sweden. We performed a genome‐scan study to detect signatures of selection potentially correlated with spatial variations in tolerance to PUUV. We analyzed six bank vole populations from Sweden, sampled from northern NE‐endemic to southern NE‐free areas. We combined candidate gene analyses ( Tlr4 , Tlr7 , and Mx2 genes) and high‐throughput sequencing of restriction site‐associated DNA (RAD) markers. Outlier loci showed high levels of genetic differentiation and significant associations with environmental data including variations in the regional number of NE human cases. Among the 108 outliers that matched to mouse protein‐coding genes, 14 corresponded to immune‐related genes. The main biological pathways found to be significantly enriched corresponded to immune processes and responses to hantavirus, including the regulation of cytokine productions, TLR cascades, and IL‐7, VEGF, and JAK–STAT signaling. In the future, genome‐scan replicates and functional ... |
format |
Article in Journal/Newspaper |
author |
Rohfritsch, Audrey Galan, Maxime Gautier, Mathieu Gharbi, Karim Olsson, Gert Gschloessl, Bernhard Zeimes, Caroline VanWambeke, Sophie Vitalis, Renaud Charbonnel, Nathalie |
spellingShingle |
Rohfritsch, Audrey Galan, Maxime Gautier, Mathieu Gharbi, Karim Olsson, Gert Gschloessl, Bernhard Zeimes, Caroline VanWambeke, Sophie Vitalis, Renaud Charbonnel, Nathalie Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden |
author_facet |
Rohfritsch, Audrey Galan, Maxime Gautier, Mathieu Gharbi, Karim Olsson, Gert Gschloessl, Bernhard Zeimes, Caroline VanWambeke, Sophie Vitalis, Renaud Charbonnel, Nathalie |
author_sort |
Rohfritsch, Audrey |
title |
Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden |
title_short |
Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden |
title_full |
Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden |
title_fullStr |
Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden |
title_full_unstemmed |
Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden |
title_sort |
preliminary insights into the genetics of bank vole tolerance to puumala hantavirus in sweden |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/ece3.4603 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.4603 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.4603 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Ecology and Evolution volume 8, issue 22, page 11273-11292 ISSN 2045-7758 2045-7758 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/ece3.4603 |
container_title |
Ecology and Evolution |
container_volume |
8 |
container_issue |
22 |
container_start_page |
11273 |
op_container_end_page |
11292 |
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1810466650435092480 |