Transgenesis in Xenopus using the Sleeping Beauty transposon system
Abstract Transposon‐based integration systems have been widely used for genetic manipulation of invertebrate and plant model systems. In the past decade, these powerful tools have begun to be used in vertebrates for transgenesis, insertional mutagenesis, and gene therapy applications. Sleeping Beaut...
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crwiley:10.1002/dvdy.21994 2024-06-02T08:03:39+00:00 Transgenesis in Xenopus using the Sleeping Beauty transposon system Yergeau, Donald A. Johnson Hamlet, Michelle R. Kuliyev, Emin Zhu, Haiqing Doherty, Joanne R. Archer, Taylor D. Subhawong, Andrea P. Valentine, Marc B. Kelley, Clair M. Mead, Paul E. 2009 http://dx.doi.org/10.1002/dvdy.21994 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fdvdy.21994 https://onlinelibrary.wiley.com/doi/full/10.1002/dvdy.21994 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Developmental Dynamics volume 238, issue 7, page 1727-1743 ISSN 1058-8388 1097-0177 journal-article 2009 crwiley https://doi.org/10.1002/dvdy.21994 2024-05-03T10:40:41Z Abstract Transposon‐based integration systems have been widely used for genetic manipulation of invertebrate and plant model systems. In the past decade, these powerful tools have begun to be used in vertebrates for transgenesis, insertional mutagenesis, and gene therapy applications. Sleeping Beauty ( SB ) is a member of Tc1/mariner class of transposases and is derived from an inactive form of the gene isolated from Atlantic salmon. SB has been used extensively in human cell lines and in whole animal vertebrate model systems such as the mouse, rat, and zebrafish. In this study, we describe the use of SB in the diploid frog Xenopus tropicalis to generate stable transgenic lines. SB transposon transgenes integrate into the X. tropicalis genome by a noncanonical process and are passed through the germline. We compare the activity of SB in this model organism with that of Tol2 , a hAT (hobo, Ac1, TAM) ‐like transposon system. Developmental Dynamics 238:1727–1743, 2009. © 2009 Wiley‐Liss, Inc. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Developmental Dynamics 238 7 1727 1743 |
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Wiley Online Library |
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English |
description |
Abstract Transposon‐based integration systems have been widely used for genetic manipulation of invertebrate and plant model systems. In the past decade, these powerful tools have begun to be used in vertebrates for transgenesis, insertional mutagenesis, and gene therapy applications. Sleeping Beauty ( SB ) is a member of Tc1/mariner class of transposases and is derived from an inactive form of the gene isolated from Atlantic salmon. SB has been used extensively in human cell lines and in whole animal vertebrate model systems such as the mouse, rat, and zebrafish. In this study, we describe the use of SB in the diploid frog Xenopus tropicalis to generate stable transgenic lines. SB transposon transgenes integrate into the X. tropicalis genome by a noncanonical process and are passed through the germline. We compare the activity of SB in this model organism with that of Tol2 , a hAT (hobo, Ac1, TAM) ‐like transposon system. Developmental Dynamics 238:1727–1743, 2009. © 2009 Wiley‐Liss, Inc. |
format |
Article in Journal/Newspaper |
author |
Yergeau, Donald A. Johnson Hamlet, Michelle R. Kuliyev, Emin Zhu, Haiqing Doherty, Joanne R. Archer, Taylor D. Subhawong, Andrea P. Valentine, Marc B. Kelley, Clair M. Mead, Paul E. |
spellingShingle |
Yergeau, Donald A. Johnson Hamlet, Michelle R. Kuliyev, Emin Zhu, Haiqing Doherty, Joanne R. Archer, Taylor D. Subhawong, Andrea P. Valentine, Marc B. Kelley, Clair M. Mead, Paul E. Transgenesis in Xenopus using the Sleeping Beauty transposon system |
author_facet |
Yergeau, Donald A. Johnson Hamlet, Michelle R. Kuliyev, Emin Zhu, Haiqing Doherty, Joanne R. Archer, Taylor D. Subhawong, Andrea P. Valentine, Marc B. Kelley, Clair M. Mead, Paul E. |
author_sort |
Yergeau, Donald A. |
title |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_short |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_full |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_fullStr |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_full_unstemmed |
Transgenesis in Xenopus using the Sleeping Beauty transposon system |
title_sort |
transgenesis in xenopus using the sleeping beauty transposon system |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1002/dvdy.21994 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fdvdy.21994 https://onlinelibrary.wiley.com/doi/full/10.1002/dvdy.21994 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Developmental Dynamics volume 238, issue 7, page 1727-1743 ISSN 1058-8388 1097-0177 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/dvdy.21994 |
container_title |
Developmental Dynamics |
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238 |
container_issue |
7 |
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1727 |
op_container_end_page |
1743 |
_version_ |
1800748246043197440 |