DNA content in eurasian sturgeon species determined by flow cytometry
Abstract The nuclear DNA content in 10 species of chondrostean fishes was measured by flow cytometry. The sterlet Acipenser ruthenus blood cells were used as an internal standard. The sterlet DNA content was calculated on the basis of comparison with the Xenopus laevis blood cells, 2C = 6.30 pg. In...
Published in: | Cytometry |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1993
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/cyto.990140406 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcyto.990140406 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990140406 |
id |
crwiley:10.1002/cyto.990140406 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/cyto.990140406 2024-09-15T18:32:55+00:00 DNA content in eurasian sturgeon species determined by flow cytometry Birstein, Vadim J. Poletaev, Andrei I. Goncharov, Boris F. 1993 http://dx.doi.org/10.1002/cyto.990140406 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcyto.990140406 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990140406 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Cytometry volume 14, issue 4, page 377-383 ISSN 0196-4763 1097-0320 journal-article 1993 crwiley https://doi.org/10.1002/cyto.990140406 2024-07-04T04:30:35Z Abstract The nuclear DNA content in 10 species of chondrostean fishes was measured by flow cytometry. The sterlet Acipenser ruthenus blood cells were used as an internal standard. The sterlet DNA content was calculated on the basis of comparison with the Xenopus laevis blood cells, 2C = 6.30 pg. In the tetraploid A. ruthenus and A. stellatus the DNA content comprises 3.74 pg/nucleus and is practically invariant; in Huso dauricus it is almost the same, 3.74–3.81 pg; and in A. nudiventris it is a little higher, 3.88–4.04 pg. In the oldest chondrostean, Pseudoscaphirhynchus kaufmanni , the nuclear DNA content is slightly lower, 2C = 3.46–3.48 pg, and in the American paddlefish Polyodon spathula it is lower still, 3.17 pg. In two octoploid sturgeons, A. baeri and A. gueldenstaedti , the DNA content is twice as high as that of the sterlet, 8.29–8.31 and 7.86–7.88 pg, respectively; a very similar amount, 8.24–8.42 pg, was determined in the hybrid Huso huso × A. ruthenus . In the Sakhalin sturgeon, A. medirostris (= A. mikadoi ), the DNA content is two times higher than in the octoploids, 13.93–14.73 pg; therefore its ploidy may be 16n and the number of chromosomes could be 500. © 1993 Wiley‐Liss, Inc. Article in Journal/Newspaper Sakhalin Wiley Online Library Cytometry 14 4 377 383 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract The nuclear DNA content in 10 species of chondrostean fishes was measured by flow cytometry. The sterlet Acipenser ruthenus blood cells were used as an internal standard. The sterlet DNA content was calculated on the basis of comparison with the Xenopus laevis blood cells, 2C = 6.30 pg. In the tetraploid A. ruthenus and A. stellatus the DNA content comprises 3.74 pg/nucleus and is practically invariant; in Huso dauricus it is almost the same, 3.74–3.81 pg; and in A. nudiventris it is a little higher, 3.88–4.04 pg. In the oldest chondrostean, Pseudoscaphirhynchus kaufmanni , the nuclear DNA content is slightly lower, 2C = 3.46–3.48 pg, and in the American paddlefish Polyodon spathula it is lower still, 3.17 pg. In two octoploid sturgeons, A. baeri and A. gueldenstaedti , the DNA content is twice as high as that of the sterlet, 8.29–8.31 and 7.86–7.88 pg, respectively; a very similar amount, 8.24–8.42 pg, was determined in the hybrid Huso huso × A. ruthenus . In the Sakhalin sturgeon, A. medirostris (= A. mikadoi ), the DNA content is two times higher than in the octoploids, 13.93–14.73 pg; therefore its ploidy may be 16n and the number of chromosomes could be 500. © 1993 Wiley‐Liss, Inc. |
format |
Article in Journal/Newspaper |
author |
Birstein, Vadim J. Poletaev, Andrei I. Goncharov, Boris F. |
spellingShingle |
Birstein, Vadim J. Poletaev, Andrei I. Goncharov, Boris F. DNA content in eurasian sturgeon species determined by flow cytometry |
author_facet |
Birstein, Vadim J. Poletaev, Andrei I. Goncharov, Boris F. |
author_sort |
Birstein, Vadim J. |
title |
DNA content in eurasian sturgeon species determined by flow cytometry |
title_short |
DNA content in eurasian sturgeon species determined by flow cytometry |
title_full |
DNA content in eurasian sturgeon species determined by flow cytometry |
title_fullStr |
DNA content in eurasian sturgeon species determined by flow cytometry |
title_full_unstemmed |
DNA content in eurasian sturgeon species determined by flow cytometry |
title_sort |
dna content in eurasian sturgeon species determined by flow cytometry |
publisher |
Wiley |
publishDate |
1993 |
url |
http://dx.doi.org/10.1002/cyto.990140406 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcyto.990140406 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990140406 |
genre |
Sakhalin |
genre_facet |
Sakhalin |
op_source |
Cytometry volume 14, issue 4, page 377-383 ISSN 0196-4763 1097-0320 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cyto.990140406 |
container_title |
Cytometry |
container_volume |
14 |
container_issue |
4 |
container_start_page |
377 |
op_container_end_page |
383 |
_version_ |
1810474676101578752 |