Genetic stock assessment of spawning Arctic cisco ( Coregonus autumnalis) populations by flow cytometric determination of DNA content
Abstract Intraspecific variation in cellular DNA content was measured in five Coregonus autumnalis spawning populations from the Mackenzie River drainage, Canada, using flow cytometry. The rivers assayed were the Peel, Arctic Red, Mountain, Carcajou, and Liard rivers. DNA content was determined from...
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crwiley:10.1002/cyto.990120309 2024-06-02T08:00:07+00:00 Genetic stock assessment of spawning Arctic cisco ( Coregonus autumnalis) populations by flow cytometric determination of DNA content Lockwood, Samuel F. Bickham, John W. 1991 http://dx.doi.org/10.1002/cyto.990120309 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcyto.990120309 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990120309 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Cytometry volume 12, issue 3, page 260-267 ISSN 0196-4763 1097-0320 journal-article 1991 crwiley https://doi.org/10.1002/cyto.990120309 2024-05-03T11:51:51Z Abstract Intraspecific variation in cellular DNA content was measured in five Coregonus autumnalis spawning populations from the Mackenzie River drainage, Canada, using flow cytometry. The rivers assayed were the Peel, Arctic Red, Mountain, Carcajou, and Liard rivers. DNA content was determined from whole blood preparations of fish from all rivers except the Carcajou, for which kidney tissue was used. DNA content measurements of kidney and blood preparations of the same fish from the Mountain River revealed statistically indistinguishable results. Mosaicism was found in blood preparations from the Peel, Arctic Red, Mountain, and Liard rivers, but was not observed in kidney tissue preparations from the Mountain or Carcajou rivers. The Liard River sample had significantly elevated mean DNA content relative to the other four samples; all other samples were statistically indistinguishable. Significant differences in mean DNA content among spawning stocks of a single species reinforces the need for adequate sample sizes of both individuals and populations when reporting āCā values for a particular species. Article in Journal/Newspaper Arctic cisco Arctic Coregonus autumnalis Liard River Mackenzie river Wiley Online Library Arctic Mackenzie River Canada Liard ENVELOPE(-67.417,-67.417,-66.850,-66.850) Carcajou ENVELOPE(-117.040,-117.040,57.767,57.767) Cytometry 12 3 260 267 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Intraspecific variation in cellular DNA content was measured in five Coregonus autumnalis spawning populations from the Mackenzie River drainage, Canada, using flow cytometry. The rivers assayed were the Peel, Arctic Red, Mountain, Carcajou, and Liard rivers. DNA content was determined from whole blood preparations of fish from all rivers except the Carcajou, for which kidney tissue was used. DNA content measurements of kidney and blood preparations of the same fish from the Mountain River revealed statistically indistinguishable results. Mosaicism was found in blood preparations from the Peel, Arctic Red, Mountain, and Liard rivers, but was not observed in kidney tissue preparations from the Mountain or Carcajou rivers. The Liard River sample had significantly elevated mean DNA content relative to the other four samples; all other samples were statistically indistinguishable. Significant differences in mean DNA content among spawning stocks of a single species reinforces the need for adequate sample sizes of both individuals and populations when reporting āCā values for a particular species. |
format |
Article in Journal/Newspaper |
author |
Lockwood, Samuel F. Bickham, John W. |
spellingShingle |
Lockwood, Samuel F. Bickham, John W. Genetic stock assessment of spawning Arctic cisco ( Coregonus autumnalis) populations by flow cytometric determination of DNA content |
author_facet |
Lockwood, Samuel F. Bickham, John W. |
author_sort |
Lockwood, Samuel F. |
title |
Genetic stock assessment of spawning Arctic cisco ( Coregonus autumnalis) populations by flow cytometric determination of DNA content |
title_short |
Genetic stock assessment of spawning Arctic cisco ( Coregonus autumnalis) populations by flow cytometric determination of DNA content |
title_full |
Genetic stock assessment of spawning Arctic cisco ( Coregonus autumnalis) populations by flow cytometric determination of DNA content |
title_fullStr |
Genetic stock assessment of spawning Arctic cisco ( Coregonus autumnalis) populations by flow cytometric determination of DNA content |
title_full_unstemmed |
Genetic stock assessment of spawning Arctic cisco ( Coregonus autumnalis) populations by flow cytometric determination of DNA content |
title_sort |
genetic stock assessment of spawning arctic cisco ( coregonus autumnalis) populations by flow cytometric determination of dna content |
publisher |
Wiley |
publishDate |
1991 |
url |
http://dx.doi.org/10.1002/cyto.990120309 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcyto.990120309 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990120309 |
long_lat |
ENVELOPE(-67.417,-67.417,-66.850,-66.850) ENVELOPE(-117.040,-117.040,57.767,57.767) |
geographic |
Arctic Mackenzie River Canada Liard Carcajou |
geographic_facet |
Arctic Mackenzie River Canada Liard Carcajou |
genre |
Arctic cisco Arctic Coregonus autumnalis Liard River Mackenzie river |
genre_facet |
Arctic cisco Arctic Coregonus autumnalis Liard River Mackenzie river |
op_source |
Cytometry volume 12, issue 3, page 260-267 ISSN 0196-4763 1097-0320 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cyto.990120309 |
container_title |
Cytometry |
container_volume |
12 |
container_issue |
3 |
container_start_page |
260 |
op_container_end_page |
267 |
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1800744124393979904 |