Kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high‐resolution flow cytometry
Abstract The two primary kinetic constants for describing the concentration dependency of nutrient uptake by microorganisms are shown to be maximal rate of substrate uptake and, rather than the Michaelis constant for transport, specific affinity. Of the two, the specific affinity is more important f...
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crwiley:10.1002/cyto.990100511 2024-06-02T08:02:15+00:00 Kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high‐resolution flow cytometry Button, D. K. Robertson, B. R. 1989 http://dx.doi.org/10.1002/cyto.990100511 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcyto.990100511 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990100511 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Cytometry volume 10, issue 5, page 558-563 ISSN 0196-4763 1097-0320 journal-article 1989 crwiley https://doi.org/10.1002/cyto.990100511 2024-05-03T12:06:46Z Abstract The two primary kinetic constants for describing the concentration dependency of nutrient uptake by microorganisms are shown to be maximal rate of substrate uptake and, rather than the Michaelis constant for transport, specific affinity. Of the two, the specific affinity is more important for describing natural aquatic microbial processes because it can be used independently at small substrate concentrations. Flow cytometry was used to evaluate specific affinities in natural populations of aquatic bacteria because it gives a convenient measure of biomass, which is an essential measurement in the specific‐affinity approach to microbial kinetics. Total biomass, biomass in various filter fractions, and the specific affinity of the bacteria in each fraction were determined in samples from a near‐arctic lake. The partial growth rate of the pelagic bacteria from the 25 μg/liter of dissolved amino acids present (growth rate from the amino acid fraction alone) was determined to be 0.78 per day. By measuring activity in screened and whole‐system populations, the biomass of the bacteria associated with particles was computed to be 427 μg/liter. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Cytometry 10 5 558 563 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The two primary kinetic constants for describing the concentration dependency of nutrient uptake by microorganisms are shown to be maximal rate of substrate uptake and, rather than the Michaelis constant for transport, specific affinity. Of the two, the specific affinity is more important for describing natural aquatic microbial processes because it can be used independently at small substrate concentrations. Flow cytometry was used to evaluate specific affinities in natural populations of aquatic bacteria because it gives a convenient measure of biomass, which is an essential measurement in the specific‐affinity approach to microbial kinetics. Total biomass, biomass in various filter fractions, and the specific affinity of the bacteria in each fraction were determined in samples from a near‐arctic lake. The partial growth rate of the pelagic bacteria from the 25 μg/liter of dissolved amino acids present (growth rate from the amino acid fraction alone) was determined to be 0.78 per day. By measuring activity in screened and whole‐system populations, the biomass of the bacteria associated with particles was computed to be 427 μg/liter. |
format |
Article in Journal/Newspaper |
author |
Button, D. K. Robertson, B. R. |
spellingShingle |
Button, D. K. Robertson, B. R. Kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high‐resolution flow cytometry |
author_facet |
Button, D. K. Robertson, B. R. |
author_sort |
Button, D. K. |
title |
Kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high‐resolution flow cytometry |
title_short |
Kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high‐resolution flow cytometry |
title_full |
Kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high‐resolution flow cytometry |
title_fullStr |
Kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high‐resolution flow cytometry |
title_full_unstemmed |
Kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high‐resolution flow cytometry |
title_sort |
kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high‐resolution flow cytometry |
publisher |
Wiley |
publishDate |
1989 |
url |
http://dx.doi.org/10.1002/cyto.990100511 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcyto.990100511 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990100511 |
long_lat |
ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Arctic Arctic Lake |
geographic_facet |
Arctic Arctic Lake |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Cytometry volume 10, issue 5, page 558-563 ISSN 0196-4763 1097-0320 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cyto.990100511 |
container_title |
Cytometry |
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10 |
container_issue |
5 |
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558 |
op_container_end_page |
563 |
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1800746763645091840 |