Cross‐Linked Enzyme‐Adhered Nanoparticles (CLEANs) for Continuous‐Flow Bioproduction
Abstract Nanostructured but micro‐sized biocatalysts were created by bottom‐up technology using multi‐functionalized silica nanoparticles (NPs) as nano‐sized building blocks to form cross‐linked enzyme‐adhered nanoparticles (CLEANs) as robust micro‐sized particles with beneficial internal structure...
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crwiley:10.1002/cssc.202102284 2024-09-15T17:40:14+00:00 Cross‐Linked Enzyme‐Adhered Nanoparticles (CLEANs) for Continuous‐Flow Bioproduction Nagy, Flóra Sánta‐Bell, Evelin Jipa, Monica Hornyánszky, Gábor Szilágyi, András László, Krisztina Katona, Gabriel Paizs, Csaba Poppe, László Balogh‐Weiser, Diána Budapesti Műszaki és Gazdaságtudományi Egyetem Magyar Tudományos Akadémia Emberi Eroforrások Minisztériuma 2021 http://dx.doi.org/10.1002/cssc.202102284 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cssc.202102284 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cssc.202102284 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor ChemSusChem volume 15, issue 2 ISSN 1864-5631 1864-564X journal-article 2021 crwiley https://doi.org/10.1002/cssc.202102284 2024-08-09T04:30:26Z Abstract Nanostructured but micro‐sized biocatalysts were created by bottom‐up technology using multi‐functionalized silica nanoparticles (NPs) as nano‐sized building blocks to form cross‐linked enzyme‐adhered nanoparticles (CLEANs) as robust micro‐sized particles with beneficial internal structure and good mechanical properties. Systematic surface modification of NPs with a grafting mixture consisting of organosilanes with reactive (aminopropyl) and inert (e. g., vinyl, propyl, phenyl, or octyl) functions resulted in functional NPs enabling cross‐linking agents, such as glutardialdehyde or bisepoxides (glycerol diglycidyl ether, neopentylglycol diglycidyl ether, and poly(propylene glycol) diglycidyl ether), to bind and cross‐link enzymes covalently and to form macroporous microparticles. These CLEANs were able to diminish several weaknesses of traditional cross‐linked enzyme aggregates as biocatalysts, such as poor mechanical resistance, difficult recovery, and storage, strengthening their use for packed‐bed enzyme reactors. Lipase B from Candida antarctica (CaLB) was selected as model enzyme for development of robust CLEANs, which were successfully tested for various industrially relevant applications including a kinetic resolution of a racemic alcohol and the production of various natural fragrance compounds under continuous‐flow conditions. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library ChemSusChem 15 2 |
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English |
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Abstract Nanostructured but micro‐sized biocatalysts were created by bottom‐up technology using multi‐functionalized silica nanoparticles (NPs) as nano‐sized building blocks to form cross‐linked enzyme‐adhered nanoparticles (CLEANs) as robust micro‐sized particles with beneficial internal structure and good mechanical properties. Systematic surface modification of NPs with a grafting mixture consisting of organosilanes with reactive (aminopropyl) and inert (e. g., vinyl, propyl, phenyl, or octyl) functions resulted in functional NPs enabling cross‐linking agents, such as glutardialdehyde or bisepoxides (glycerol diglycidyl ether, neopentylglycol diglycidyl ether, and poly(propylene glycol) diglycidyl ether), to bind and cross‐link enzymes covalently and to form macroporous microparticles. These CLEANs were able to diminish several weaknesses of traditional cross‐linked enzyme aggregates as biocatalysts, such as poor mechanical resistance, difficult recovery, and storage, strengthening their use for packed‐bed enzyme reactors. Lipase B from Candida antarctica (CaLB) was selected as model enzyme for development of robust CLEANs, which were successfully tested for various industrially relevant applications including a kinetic resolution of a racemic alcohol and the production of various natural fragrance compounds under continuous‐flow conditions. |
author2 |
Budapesti Műszaki és Gazdaságtudományi Egyetem Magyar Tudományos Akadémia Emberi Eroforrások Minisztériuma |
format |
Article in Journal/Newspaper |
author |
Nagy, Flóra Sánta‐Bell, Evelin Jipa, Monica Hornyánszky, Gábor Szilágyi, András László, Krisztina Katona, Gabriel Paizs, Csaba Poppe, László Balogh‐Weiser, Diána |
spellingShingle |
Nagy, Flóra Sánta‐Bell, Evelin Jipa, Monica Hornyánszky, Gábor Szilágyi, András László, Krisztina Katona, Gabriel Paizs, Csaba Poppe, László Balogh‐Weiser, Diána Cross‐Linked Enzyme‐Adhered Nanoparticles (CLEANs) for Continuous‐Flow Bioproduction |
author_facet |
Nagy, Flóra Sánta‐Bell, Evelin Jipa, Monica Hornyánszky, Gábor Szilágyi, András László, Krisztina Katona, Gabriel Paizs, Csaba Poppe, László Balogh‐Weiser, Diána |
author_sort |
Nagy, Flóra |
title |
Cross‐Linked Enzyme‐Adhered Nanoparticles (CLEANs) for Continuous‐Flow Bioproduction |
title_short |
Cross‐Linked Enzyme‐Adhered Nanoparticles (CLEANs) for Continuous‐Flow Bioproduction |
title_full |
Cross‐Linked Enzyme‐Adhered Nanoparticles (CLEANs) for Continuous‐Flow Bioproduction |
title_fullStr |
Cross‐Linked Enzyme‐Adhered Nanoparticles (CLEANs) for Continuous‐Flow Bioproduction |
title_full_unstemmed |
Cross‐Linked Enzyme‐Adhered Nanoparticles (CLEANs) for Continuous‐Flow Bioproduction |
title_sort |
cross‐linked enzyme‐adhered nanoparticles (cleans) for continuous‐flow bioproduction |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1002/cssc.202102284 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cssc.202102284 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cssc.202102284 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ChemSusChem volume 15, issue 2 ISSN 1864-5631 1864-564X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cssc.202102284 |
container_title |
ChemSusChem |
container_volume |
15 |
container_issue |
2 |
_version_ |
1810485847550590976 |