Tailoring Particle‐Enzyme Nanoconjugates for Biocatalysis at the Organic‐Organic Interface
Abstract Nonaqueous Pickering emulsions (PEs) are a powerful platform for catalysis design, offering both a large interface contact and a preferable environment for water‐sensitive synthesis. However, up to now, little progress has been made to incorporate insoluble enzymes into the nonaqueous syste...
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crwiley:10.1002/cssc.202002121 2024-05-19T07:32:31+00:00 Tailoring Particle‐Enzyme Nanoconjugates for Biocatalysis at the Organic‐Organic Interface Sun, Zhiyong Cai, Meng Hübner, René Ansorge‐Schumacher, Marion B. Wu, Changzhu Deutsche Forschungsgemeinschaft 2020 http://dx.doi.org/10.1002/cssc.202002121 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cssc.202002121 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cssc.202002121 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor ChemSusChem volume 13, issue 24, page 6523-6527 ISSN 1864-5631 1864-564X journal-article 2020 crwiley https://doi.org/10.1002/cssc.202002121 2024-04-25T08:26:00Z Abstract Nonaqueous Pickering emulsions (PEs) are a powerful platform for catalysis design, offering both a large interface contact and a preferable environment for water‐sensitive synthesis. However, up to now, little progress has been made to incorporate insoluble enzymes into the nonaqueous system for biotransformation. Herein, we present biocatalytically active nonaqueous PEs, stabilized by particle‐enzyme nanoconjugates, for the fast transesterification and esterification, and eventually for biodiesel synthesis. Our nanoconjugates are the hybrid biocatalysts tailor‐made by loading hydrophilic Candida antarctica lipase B onto hydrophobic silica nanoparticles, resulting in not only catalytically active but highly amphiphilic particles for stabilization of a methanol‐decane emulsion. The enzyme activity in these PEs is significantly enhanced, ca. 375‐fold higher than in the nonaqueous biphasic control. Moreover, the PEs can be multiply reused without significant loss of enzyme performance. With this proof‐of‐concept, this system can be expanded for many advanced syntheses using different enzymes in the future. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library ChemSusChem 13 24 6523 6527 |
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Wiley Online Library |
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English |
description |
Abstract Nonaqueous Pickering emulsions (PEs) are a powerful platform for catalysis design, offering both a large interface contact and a preferable environment for water‐sensitive synthesis. However, up to now, little progress has been made to incorporate insoluble enzymes into the nonaqueous system for biotransformation. Herein, we present biocatalytically active nonaqueous PEs, stabilized by particle‐enzyme nanoconjugates, for the fast transesterification and esterification, and eventually for biodiesel synthesis. Our nanoconjugates are the hybrid biocatalysts tailor‐made by loading hydrophilic Candida antarctica lipase B onto hydrophobic silica nanoparticles, resulting in not only catalytically active but highly amphiphilic particles for stabilization of a methanol‐decane emulsion. The enzyme activity in these PEs is significantly enhanced, ca. 375‐fold higher than in the nonaqueous biphasic control. Moreover, the PEs can be multiply reused without significant loss of enzyme performance. With this proof‐of‐concept, this system can be expanded for many advanced syntheses using different enzymes in the future. |
author2 |
Deutsche Forschungsgemeinschaft |
format |
Article in Journal/Newspaper |
author |
Sun, Zhiyong Cai, Meng Hübner, René Ansorge‐Schumacher, Marion B. Wu, Changzhu |
spellingShingle |
Sun, Zhiyong Cai, Meng Hübner, René Ansorge‐Schumacher, Marion B. Wu, Changzhu Tailoring Particle‐Enzyme Nanoconjugates for Biocatalysis at the Organic‐Organic Interface |
author_facet |
Sun, Zhiyong Cai, Meng Hübner, René Ansorge‐Schumacher, Marion B. Wu, Changzhu |
author_sort |
Sun, Zhiyong |
title |
Tailoring Particle‐Enzyme Nanoconjugates for Biocatalysis at the Organic‐Organic Interface |
title_short |
Tailoring Particle‐Enzyme Nanoconjugates for Biocatalysis at the Organic‐Organic Interface |
title_full |
Tailoring Particle‐Enzyme Nanoconjugates for Biocatalysis at the Organic‐Organic Interface |
title_fullStr |
Tailoring Particle‐Enzyme Nanoconjugates for Biocatalysis at the Organic‐Organic Interface |
title_full_unstemmed |
Tailoring Particle‐Enzyme Nanoconjugates for Biocatalysis at the Organic‐Organic Interface |
title_sort |
tailoring particle‐enzyme nanoconjugates for biocatalysis at the organic‐organic interface |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1002/cssc.202002121 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cssc.202002121 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cssc.202002121 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ChemSusChem volume 13, issue 24, page 6523-6527 ISSN 1864-5631 1864-564X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cssc.202002121 |
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ChemSusChem |
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13 |
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24 |
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6527 |
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1799470585924812800 |