Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout
Abstract The decapeptide gonadotropin‐releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. The distribution of salmon GnRH (sGnRH), which is the major form in salmonids, has been studied in different fish species by immunocytochemistry. Discrepancies in data concerni...
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crwiley:10.1002/cne.903470310 2024-10-13T14:06:00+00:00 Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout Bailhache, Thierry Arazam, Aïcha Klungland, Helge Aleström, Peter Breton, Bernard Jego, Patrick 1994 http://dx.doi.org/10.1002/cne.903470310 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcne.903470310 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cne.903470310 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Comparative Neurology volume 347, issue 3, page 444-454 ISSN 0021-9967 1096-9861 journal-article 1994 crwiley https://doi.org/10.1002/cne.903470310 2024-09-17T04:47:07Z Abstract The decapeptide gonadotropin‐releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. The distribution of salmon GnRH (sGnRH), which is the major form in salmonids, has been studied in different fish species by immunocytochemistry. Discrepancies in data concerning the distribution of sGnRH perikarya led us to investigate this problem in two species, the Atlantic salmon and the rainbow trout, with in situ hybridization of sGnRH messenger, a highly specific molecular tool. By Northern blot analysis, the rainbow trout sGnRH messenger appears to be about 500 bases in length, which is close to those isolated from Atlantic salmon or masu salmon and characterized previously, In situ hybridization with riboprobes generated with Atlantic salmon sGnRH cDNA demonstrated that sGnRH perikarya are restricted to the ventral part of olfactory bulbs, telencephalon, and preoptic area. They are distributed on a nearly continuous line extending from the olfactory bulbs to the preoptic area in both salmonid species studied. Despite the presence of GnRH‐like immunoreactivity in the preoptic magnocellular nucleus (NPOm) and in the tegmentum of the midbrain (MT), the sGnRH mRNA is not present in these two structures. Stained cells in NPOm could be target cells for GnRH and immunoreactive neurons in MT are likely to be chicken GnRH‐II containing cells. Our study not only gives a precise distribution of the sGnRH system in two salmonids, Atlantic salmon and rainbow trout, but also clarifies the ambiguous data published up to now in rainbow trout. © 1994 Wiley‐Liss, Inc. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Journal of Comparative Neurology 347 3 444 454 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The decapeptide gonadotropin‐releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. The distribution of salmon GnRH (sGnRH), which is the major form in salmonids, has been studied in different fish species by immunocytochemistry. Discrepancies in data concerning the distribution of sGnRH perikarya led us to investigate this problem in two species, the Atlantic salmon and the rainbow trout, with in situ hybridization of sGnRH messenger, a highly specific molecular tool. By Northern blot analysis, the rainbow trout sGnRH messenger appears to be about 500 bases in length, which is close to those isolated from Atlantic salmon or masu salmon and characterized previously, In situ hybridization with riboprobes generated with Atlantic salmon sGnRH cDNA demonstrated that sGnRH perikarya are restricted to the ventral part of olfactory bulbs, telencephalon, and preoptic area. They are distributed on a nearly continuous line extending from the olfactory bulbs to the preoptic area in both salmonid species studied. Despite the presence of GnRH‐like immunoreactivity in the preoptic magnocellular nucleus (NPOm) and in the tegmentum of the midbrain (MT), the sGnRH mRNA is not present in these two structures. Stained cells in NPOm could be target cells for GnRH and immunoreactive neurons in MT are likely to be chicken GnRH‐II containing cells. Our study not only gives a precise distribution of the sGnRH system in two salmonids, Atlantic salmon and rainbow trout, but also clarifies the ambiguous data published up to now in rainbow trout. © 1994 Wiley‐Liss, Inc. |
format |
Article in Journal/Newspaper |
author |
Bailhache, Thierry Arazam, Aïcha Klungland, Helge Aleström, Peter Breton, Bernard Jego, Patrick |
spellingShingle |
Bailhache, Thierry Arazam, Aïcha Klungland, Helge Aleström, Peter Breton, Bernard Jego, Patrick Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout |
author_facet |
Bailhache, Thierry Arazam, Aïcha Klungland, Helge Aleström, Peter Breton, Bernard Jego, Patrick |
author_sort |
Bailhache, Thierry |
title |
Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout |
title_short |
Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout |
title_full |
Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout |
title_fullStr |
Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout |
title_full_unstemmed |
Localization of salmon gonadotropin‐releasing hormone mRNA and peptide in the brain of atlantic salmon and rainbow trout |
title_sort |
localization of salmon gonadotropin‐releasing hormone mrna and peptide in the brain of atlantic salmon and rainbow trout |
publisher |
Wiley |
publishDate |
1994 |
url |
http://dx.doi.org/10.1002/cne.903470310 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcne.903470310 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cne.903470310 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Comparative Neurology volume 347, issue 3, page 444-454 ISSN 0021-9967 1096-9861 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cne.903470310 |
container_title |
Journal of Comparative Neurology |
container_volume |
347 |
container_issue |
3 |
container_start_page |
444 |
op_container_end_page |
454 |
_version_ |
1812812034961571840 |