Mapping key neuropeptides involved in the melanocortin system in Atlantic salmon ( Salmo salar ) brain
Abstract The melanocortin system is a key regulator of appetite and food intake in vertebrates. This system includes the neuropeptides neuropeptide y (NPY), agouti‐related peptide (AGRP), cocaine‐ and amphetamine‐regulated transcript (CART), and pro‐opiomelanocortin (POMC). An important center for a...
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crwiley:10.1002/cne.25415 2024-04-28T08:13:21+00:00 Mapping key neuropeptides involved in the melanocortin system in Atlantic salmon ( Salmo salar ) brain Norland, Sissel Eilertsen, Mariann Rønnestad, Ivar Helvik, Jon Vidar Gomes, Ana S. Norges Forskningsråd 2022 http://dx.doi.org/10.1002/cne.25415 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cne.25415 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cne.25415 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Journal of Comparative Neurology volume 531, issue 1, page 89-115 ISSN 0021-9967 1096-9861 General Neuroscience journal-article 2022 crwiley https://doi.org/10.1002/cne.25415 2024-04-02T08:43:52Z Abstract The melanocortin system is a key regulator of appetite and food intake in vertebrates. This system includes the neuropeptides neuropeptide y (NPY), agouti‐related peptide (AGRP), cocaine‐ and amphetamine‐regulated transcript (CART), and pro‐opiomelanocortin (POMC). An important center for appetite control in mammals is the hypothalamic arcuate nucleus, with neurons that coexpress either the orexigenic NPY/AGRP or the anorexigenic CART/POMC neuropeptides. In ray‐finned fishes, such a center is less characterized. The Atlantic salmon ( Salmo salar ) has multiple genes of these neuropeptides due to whole‐genome duplication events. To better understand the potential involvement of the melanocortin system in appetite and food intake control, we have mapped the mRNA expression of npy , agrp , cart , and pomc in the brain of Atlantic salmon parr using in situ hybridization. After identifying hypothalamic mRNA expression, we investigated the possible intracellular coexpression of npy/agrp and cart/pomc in the tuberal hypothalamus by fluorescent in situ hybridization. The results showed that the neuropeptides were widely distributed, especially in sensory and neuroendocrine brain regions. In the hypothalamic lateral tuberal nucleus, the putative homolog to the mammalian arcuate nucleus, npya , agrp1 , cart2b , and pomca were predominantly localized in distinct neurons; however, some neurons coexpressed cart2b/pomca . This is the first demonstration of coexpression of cart2b/pomca in the tuberal hypothalamus of a teleost. Collectively, our data suggest that the lateral tuberal nucleus is the center for appetite control in salmon, similar to that of mammals. Extrahypothalamic brain regions might also be involved in regulating food intake, including the olfactory bulb, telencephalon, midbrain, and hindbrain. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Comparative Neurology 531 1 89 115 |
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Wiley Online Library |
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language |
English |
topic |
General Neuroscience |
spellingShingle |
General Neuroscience Norland, Sissel Eilertsen, Mariann Rønnestad, Ivar Helvik, Jon Vidar Gomes, Ana S. Mapping key neuropeptides involved in the melanocortin system in Atlantic salmon ( Salmo salar ) brain |
topic_facet |
General Neuroscience |
description |
Abstract The melanocortin system is a key regulator of appetite and food intake in vertebrates. This system includes the neuropeptides neuropeptide y (NPY), agouti‐related peptide (AGRP), cocaine‐ and amphetamine‐regulated transcript (CART), and pro‐opiomelanocortin (POMC). An important center for appetite control in mammals is the hypothalamic arcuate nucleus, with neurons that coexpress either the orexigenic NPY/AGRP or the anorexigenic CART/POMC neuropeptides. In ray‐finned fishes, such a center is less characterized. The Atlantic salmon ( Salmo salar ) has multiple genes of these neuropeptides due to whole‐genome duplication events. To better understand the potential involvement of the melanocortin system in appetite and food intake control, we have mapped the mRNA expression of npy , agrp , cart , and pomc in the brain of Atlantic salmon parr using in situ hybridization. After identifying hypothalamic mRNA expression, we investigated the possible intracellular coexpression of npy/agrp and cart/pomc in the tuberal hypothalamus by fluorescent in situ hybridization. The results showed that the neuropeptides were widely distributed, especially in sensory and neuroendocrine brain regions. In the hypothalamic lateral tuberal nucleus, the putative homolog to the mammalian arcuate nucleus, npya , agrp1 , cart2b , and pomca were predominantly localized in distinct neurons; however, some neurons coexpressed cart2b/pomca . This is the first demonstration of coexpression of cart2b/pomca in the tuberal hypothalamus of a teleost. Collectively, our data suggest that the lateral tuberal nucleus is the center for appetite control in salmon, similar to that of mammals. Extrahypothalamic brain regions might also be involved in regulating food intake, including the olfactory bulb, telencephalon, midbrain, and hindbrain. |
author2 |
Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Norland, Sissel Eilertsen, Mariann Rønnestad, Ivar Helvik, Jon Vidar Gomes, Ana S. |
author_facet |
Norland, Sissel Eilertsen, Mariann Rønnestad, Ivar Helvik, Jon Vidar Gomes, Ana S. |
author_sort |
Norland, Sissel |
title |
Mapping key neuropeptides involved in the melanocortin system in Atlantic salmon ( Salmo salar ) brain |
title_short |
Mapping key neuropeptides involved in the melanocortin system in Atlantic salmon ( Salmo salar ) brain |
title_full |
Mapping key neuropeptides involved in the melanocortin system in Atlantic salmon ( Salmo salar ) brain |
title_fullStr |
Mapping key neuropeptides involved in the melanocortin system in Atlantic salmon ( Salmo salar ) brain |
title_full_unstemmed |
Mapping key neuropeptides involved in the melanocortin system in Atlantic salmon ( Salmo salar ) brain |
title_sort |
mapping key neuropeptides involved in the melanocortin system in atlantic salmon ( salmo salar ) brain |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1002/cne.25415 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cne.25415 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cne.25415 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Comparative Neurology volume 531, issue 1, page 89-115 ISSN 0021-9967 1096-9861 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/cne.25415 |
container_title |
Journal of Comparative Neurology |
container_volume |
531 |
container_issue |
1 |
container_start_page |
89 |
op_container_end_page |
115 |
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1797579891603931136 |