Muscle bioenergetics of speeding fish: In vivo 31 P‐NMR studies in a 4.7 T MR scanner with an integrated swim tunnel
Abstract Energetic studies on exercising animals are usually limited to oxygen consumption measurements in respirometers followed by invasive tissue sampling and analysis of metabolites. Noninvasive studies of exercising animals like through the use of 31 P NMR are typically restricted to “stop and...
Published in: | Concepts in Magnetic Resonance Part B: Magnetic Resonance Engineering |
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crwiley:10.1002/cmr.b.20105 2024-06-02T08:03:13+00:00 Muscle bioenergetics of speeding fish: In vivo 31 P‐NMR studies in a 4.7 T MR scanner with an integrated swim tunnel Bock, Christian Lurman, Glenn J. Wittig, Rolf‐M. Webber, Dale M. Pörtner, Hans‐O. 2008 http://dx.doi.org/10.1002/cmr.b.20105 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcmr.b.20105 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cmr.b.20105 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Concepts in Magnetic Resonance Part B: Magnetic Resonance Engineering volume 33B, issue 1, page 62-73 ISSN 1552-5031 1552-504X journal-article 2008 crwiley https://doi.org/10.1002/cmr.b.20105 2024-05-03T11:31:51Z Abstract Energetic studies on exercising animals are usually limited to oxygen consumption measurements in respirometers followed by invasive tissue sampling and analysis of metabolites. Noninvasive studies of exercising animals like through the use of 31 P NMR are typically restricted to “stop and go” measurements. Furthermore, magnetic resonance studies of marine animals are hampered by sea water, a highly electric conductive and dielectric medium, resulting in heavy loading and strong RF loss. In this work, we present a set‐up for online determination of muscle bioenergetics in swimming marine fish, Atlantic cod (Gadus morhua), using in vivo 31 P NMR spectroscopy, which overcome these limitations. Special hardware and RF coils were developed for this purpose. A birdcage resonator adapted to high loadings was used for signal excitation. An insulated inductive coil (2 cm diameter) was fixed onto the surface of the fish tail and placed opposite a watertight, passively decoupled 9 × 6 cm 2 elliptic and curved surface coil for signal recordings. This arrangement led to enhanced penetration of the RF signal and an almost 10‐fold increase in S/N ratio compared to the exclusive use of the elliptic surface coil. Monitoring of tail beat allowed to set trigger values resulted in an improved quality of in vivo 31 P NMR spectra of swimming fish. We report the first successful NMR experiments recording simultaneously tissue energetic and acid–base parameters on swimming cod depending on tail beat frequency and amplitude to determine critical swimming speeds. © 2008 Wiley Periodicals, Inc. Concepts Magn Reson Part B (Magn Reson Engineering) 33B: 62–73, 2008 Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library Concepts in Magnetic Resonance Part B: Magnetic Resonance Engineering 33B 1 62 73 |
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English |
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Abstract Energetic studies on exercising animals are usually limited to oxygen consumption measurements in respirometers followed by invasive tissue sampling and analysis of metabolites. Noninvasive studies of exercising animals like through the use of 31 P NMR are typically restricted to “stop and go” measurements. Furthermore, magnetic resonance studies of marine animals are hampered by sea water, a highly electric conductive and dielectric medium, resulting in heavy loading and strong RF loss. In this work, we present a set‐up for online determination of muscle bioenergetics in swimming marine fish, Atlantic cod (Gadus morhua), using in vivo 31 P NMR spectroscopy, which overcome these limitations. Special hardware and RF coils were developed for this purpose. A birdcage resonator adapted to high loadings was used for signal excitation. An insulated inductive coil (2 cm diameter) was fixed onto the surface of the fish tail and placed opposite a watertight, passively decoupled 9 × 6 cm 2 elliptic and curved surface coil for signal recordings. This arrangement led to enhanced penetration of the RF signal and an almost 10‐fold increase in S/N ratio compared to the exclusive use of the elliptic surface coil. Monitoring of tail beat allowed to set trigger values resulted in an improved quality of in vivo 31 P NMR spectra of swimming fish. We report the first successful NMR experiments recording simultaneously tissue energetic and acid–base parameters on swimming cod depending on tail beat frequency and amplitude to determine critical swimming speeds. © 2008 Wiley Periodicals, Inc. Concepts Magn Reson Part B (Magn Reson Engineering) 33B: 62–73, 2008 |
format |
Article in Journal/Newspaper |
author |
Bock, Christian Lurman, Glenn J. Wittig, Rolf‐M. Webber, Dale M. Pörtner, Hans‐O. |
spellingShingle |
Bock, Christian Lurman, Glenn J. Wittig, Rolf‐M. Webber, Dale M. Pörtner, Hans‐O. Muscle bioenergetics of speeding fish: In vivo 31 P‐NMR studies in a 4.7 T MR scanner with an integrated swim tunnel |
author_facet |
Bock, Christian Lurman, Glenn J. Wittig, Rolf‐M. Webber, Dale M. Pörtner, Hans‐O. |
author_sort |
Bock, Christian |
title |
Muscle bioenergetics of speeding fish: In vivo 31 P‐NMR studies in a 4.7 T MR scanner with an integrated swim tunnel |
title_short |
Muscle bioenergetics of speeding fish: In vivo 31 P‐NMR studies in a 4.7 T MR scanner with an integrated swim tunnel |
title_full |
Muscle bioenergetics of speeding fish: In vivo 31 P‐NMR studies in a 4.7 T MR scanner with an integrated swim tunnel |
title_fullStr |
Muscle bioenergetics of speeding fish: In vivo 31 P‐NMR studies in a 4.7 T MR scanner with an integrated swim tunnel |
title_full_unstemmed |
Muscle bioenergetics of speeding fish: In vivo 31 P‐NMR studies in a 4.7 T MR scanner with an integrated swim tunnel |
title_sort |
muscle bioenergetics of speeding fish: in vivo 31 p‐nmr studies in a 4.7 t mr scanner with an integrated swim tunnel |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1002/cmr.b.20105 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcmr.b.20105 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cmr.b.20105 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Concepts in Magnetic Resonance Part B: Magnetic Resonance Engineering volume 33B, issue 1, page 62-73 ISSN 1552-5031 1552-504X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cmr.b.20105 |
container_title |
Concepts in Magnetic Resonance Part B: Magnetic Resonance Engineering |
container_volume |
33B |
container_issue |
1 |
container_start_page |
62 |
op_container_end_page |
73 |
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1800747697224810496 |