Divergent neural β tubulin from the antarctic fish notothenia coriiceps neglecta: Potential sequence contributions to cold adaptation of microtubule assembly
Abstract The cytoplasmic microtubules of the cold‐adapted Antarctic fishes, unlike those of homeotherms and temperate poikilotherms, assemble and function at body temperatures in the range −1.8 to +2°C. To determine whether alterations to the primary sequence of β tubulin may contribute to enhanceme...
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crwiley:10.1002/cm.970240303 2024-06-02T07:57:47+00:00 Divergent neural β tubulin from the antarctic fish notothenia coriiceps neglecta: Potential sequence contributions to cold adaptation of microtubule assembly Detrich, H. William Parker, Sandra K. 1993 http://dx.doi.org/10.1002/cm.970240303 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcm.970240303 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cm.970240303 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Cell Motility volume 24, issue 3, page 156-166 ISSN 0886-1544 journal-article 1993 crwiley https://doi.org/10.1002/cm.970240303 2024-05-03T10:47:51Z Abstract The cytoplasmic microtubules of the cold‐adapted Antarctic fishes, unlike those of homeotherms and temperate poikilotherms, assemble and function at body temperatures in the range −1.8 to +2°C. To determine whether alterations to the primary sequence of β tubulin may contribute to enhancement of microtubule assembly at cold temperatures, we have cloned and sequenced a 1.8‐kilobase neural β‐chain cDNA, Ncnβ1, from an Antarctic rockcod, Notothenia coriiceps neglecta . Based on nucleotide sequence homology, Ncnβ1 probably corresponds to a class‐II β‐tubulin gene. The 446‐residue β chain encoded by Ncnβ1 is closely related (sequence homology ∼95%) both to the neural class‐I/II isotypes and to the neural/testicular class‐IV variants of higher vertebrates, but the sequence of its carboxy‐terminal isotype‐defining region (residues 431–446) has diverged markedly (≥ 25% change relative to the I/II/IV referents). Furthermore, the NcnβsZ1 polypeptide contains six unique amino‐acid substitutions (five conservative, one nonconservative) not found in other vertebrate brain isotypes, and the carboxyterminal region possesses a unique tyrosine inserted at position 442. We conclude that Ncnβ1 encodes a class‐II β tubulin that contains sequence modifications, located largely in its interdimer contact domain, that may contribute to cold adaptation of microtubule assembly. © 1993 Wiley‐Liss, Inc. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The Antarctic Cell Motility and the Cytoskeleton 24 3 156 166 |
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Wiley Online Library |
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English |
description |
Abstract The cytoplasmic microtubules of the cold‐adapted Antarctic fishes, unlike those of homeotherms and temperate poikilotherms, assemble and function at body temperatures in the range −1.8 to +2°C. To determine whether alterations to the primary sequence of β tubulin may contribute to enhancement of microtubule assembly at cold temperatures, we have cloned and sequenced a 1.8‐kilobase neural β‐chain cDNA, Ncnβ1, from an Antarctic rockcod, Notothenia coriiceps neglecta . Based on nucleotide sequence homology, Ncnβ1 probably corresponds to a class‐II β‐tubulin gene. The 446‐residue β chain encoded by Ncnβ1 is closely related (sequence homology ∼95%) both to the neural class‐I/II isotypes and to the neural/testicular class‐IV variants of higher vertebrates, but the sequence of its carboxy‐terminal isotype‐defining region (residues 431–446) has diverged markedly (≥ 25% change relative to the I/II/IV referents). Furthermore, the NcnβsZ1 polypeptide contains six unique amino‐acid substitutions (five conservative, one nonconservative) not found in other vertebrate brain isotypes, and the carboxyterminal region possesses a unique tyrosine inserted at position 442. We conclude that Ncnβ1 encodes a class‐II β tubulin that contains sequence modifications, located largely in its interdimer contact domain, that may contribute to cold adaptation of microtubule assembly. © 1993 Wiley‐Liss, Inc. |
format |
Article in Journal/Newspaper |
author |
Detrich, H. William Parker, Sandra K. |
spellingShingle |
Detrich, H. William Parker, Sandra K. Divergent neural β tubulin from the antarctic fish notothenia coriiceps neglecta: Potential sequence contributions to cold adaptation of microtubule assembly |
author_facet |
Detrich, H. William Parker, Sandra K. |
author_sort |
Detrich, H. William |
title |
Divergent neural β tubulin from the antarctic fish notothenia coriiceps neglecta: Potential sequence contributions to cold adaptation of microtubule assembly |
title_short |
Divergent neural β tubulin from the antarctic fish notothenia coriiceps neglecta: Potential sequence contributions to cold adaptation of microtubule assembly |
title_full |
Divergent neural β tubulin from the antarctic fish notothenia coriiceps neglecta: Potential sequence contributions to cold adaptation of microtubule assembly |
title_fullStr |
Divergent neural β tubulin from the antarctic fish notothenia coriiceps neglecta: Potential sequence contributions to cold adaptation of microtubule assembly |
title_full_unstemmed |
Divergent neural β tubulin from the antarctic fish notothenia coriiceps neglecta: Potential sequence contributions to cold adaptation of microtubule assembly |
title_sort |
divergent neural β tubulin from the antarctic fish notothenia coriiceps neglecta: potential sequence contributions to cold adaptation of microtubule assembly |
publisher |
Wiley |
publishDate |
1993 |
url |
http://dx.doi.org/10.1002/cm.970240303 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcm.970240303 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cm.970240303 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Cell Motility volume 24, issue 3, page 156-166 ISSN 0886-1544 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cm.970240303 |
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Cell Motility and the Cytoskeleton |
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24 |
container_issue |
3 |
container_start_page |
156 |
op_container_end_page |
166 |
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1800740982051831808 |