Optimised Protocol for Drying Aqueous Enzyme Solutions in Organic Solvents – Comparison of Free and Immobilised Candida antarctica Lipase B
Abstract Here, we propose an optimised protocol for controlling the initial water activity (a w ) in organic reaction mixtures with soluble lipase to ensure reproducible and consistent enzyme activity measurements. Pre‐equilibration above saturated salt solutions was used to set a w of the reaction...
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Online Access: | http://dx.doi.org/10.1002/cctc.202201207 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cctc.202201207 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cctc.202201207 |
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crwiley:10.1002/cctc.202201207 2024-06-02T07:57:21+00:00 Optimised Protocol for Drying Aqueous Enzyme Solutions in Organic Solvents – Comparison of Free and Immobilised Candida antarctica Lipase B Tjørnelund, Helena D. Brask, Jesper Woodley, John M. Peters, Günther H. J. 2022 http://dx.doi.org/10.1002/cctc.202201207 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cctc.202201207 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cctc.202201207 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ ChemCatChem volume 15, issue 1 ISSN 1867-3880 1867-3899 journal-article 2022 crwiley https://doi.org/10.1002/cctc.202201207 2024-05-03T11:27:31Z Abstract Here, we propose an optimised protocol for controlling the initial water activity (a w ) in organic reaction mixtures with soluble lipase to ensure reproducible and consistent enzyme activity measurements. Pre‐equilibration above saturated salt solutions was used to set a w of the reaction media, where a w was tracked with a hygrometer. Consistent stirring of the suspension and volume correction to compensate for solvent evaporation were found to be critical. The protocol was tested on Candida antarctica lipase B (CALB) in soluble and immobilised forms in three organic solvents (acetonitrile, methyl tert‐ butyl ether, and hexane) at four different water activities ranging from 0.12 to 0.97. Soluble and immobilised CALB had similar a w ‐profiles and showed the highest enzyme activity in hexane. The optimised protocol expands the possibility to study enzyme kinetics from immobilised enzymes to soluble enzymes. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library ChemCatChem 15 1 |
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English |
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Abstract Here, we propose an optimised protocol for controlling the initial water activity (a w ) in organic reaction mixtures with soluble lipase to ensure reproducible and consistent enzyme activity measurements. Pre‐equilibration above saturated salt solutions was used to set a w of the reaction media, where a w was tracked with a hygrometer. Consistent stirring of the suspension and volume correction to compensate for solvent evaporation were found to be critical. The protocol was tested on Candida antarctica lipase B (CALB) in soluble and immobilised forms in three organic solvents (acetonitrile, methyl tert‐ butyl ether, and hexane) at four different water activities ranging from 0.12 to 0.97. Soluble and immobilised CALB had similar a w ‐profiles and showed the highest enzyme activity in hexane. The optimised protocol expands the possibility to study enzyme kinetics from immobilised enzymes to soluble enzymes. |
format |
Article in Journal/Newspaper |
author |
Tjørnelund, Helena D. Brask, Jesper Woodley, John M. Peters, Günther H. J. |
spellingShingle |
Tjørnelund, Helena D. Brask, Jesper Woodley, John M. Peters, Günther H. J. Optimised Protocol for Drying Aqueous Enzyme Solutions in Organic Solvents – Comparison of Free and Immobilised Candida antarctica Lipase B |
author_facet |
Tjørnelund, Helena D. Brask, Jesper Woodley, John M. Peters, Günther H. J. |
author_sort |
Tjørnelund, Helena D. |
title |
Optimised Protocol for Drying Aqueous Enzyme Solutions in Organic Solvents – Comparison of Free and Immobilised Candida antarctica Lipase B |
title_short |
Optimised Protocol for Drying Aqueous Enzyme Solutions in Organic Solvents – Comparison of Free and Immobilised Candida antarctica Lipase B |
title_full |
Optimised Protocol for Drying Aqueous Enzyme Solutions in Organic Solvents – Comparison of Free and Immobilised Candida antarctica Lipase B |
title_fullStr |
Optimised Protocol for Drying Aqueous Enzyme Solutions in Organic Solvents – Comparison of Free and Immobilised Candida antarctica Lipase B |
title_full_unstemmed |
Optimised Protocol for Drying Aqueous Enzyme Solutions in Organic Solvents – Comparison of Free and Immobilised Candida antarctica Lipase B |
title_sort |
optimised protocol for drying aqueous enzyme solutions in organic solvents – comparison of free and immobilised candida antarctica lipase b |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1002/cctc.202201207 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cctc.202201207 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cctc.202201207 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ChemCatChem volume 15, issue 1 ISSN 1867-3880 1867-3899 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1002/cctc.202201207 |
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ChemCatChem |
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15 |
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1 |
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1800740502871474176 |