Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media
Abstract Literature reports biocatalytically active static emulsions (BASE) as promising systems for the preparation of biocatalysts designed for synthetic use in organic media. Their excellent catalytic performance is attributed to the numerous micropools of dissolved enzymes independently disperse...
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crwiley:10.1002/cctc.201100085 2024-09-09T19:03:00+00:00 Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media Wu, Changzhu Kraume, Matthias Ansorge‐Schumacher, Marion B. 2011 http://dx.doi.org/10.1002/cctc.201100085 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcctc.201100085 http://onlinelibrary.wiley.com/wol1/doi/10.1002/cctc.201100085/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor ChemCatChem volume 3, issue 8, page 1314-1319 ISSN 1867-3880 1867-3899 journal-article 2011 crwiley https://doi.org/10.1002/cctc.201100085 2024-06-18T04:17:03Z Abstract Literature reports biocatalytically active static emulsions (BASE) as promising systems for the preparation of biocatalysts designed for synthetic use in organic media. Their excellent catalytic performance is attributed to the numerous micropools of dissolved enzymes independently dispersed in silicone beads. Here, a systematic study of the structure and morphology of BASE and optimization in terms of bead size distribution and overall catalytic performance is presented. The study relies on beads obtained by using a novel preparation method that enables a considerably improved reproducibility of the particle size and catalytic activity in separate batches. A large interfacial area of 0.023 m 2 g −1 material was calculated. The adjustment of bead composition increased the apparent catalytic activity of entrapped lipase A from Candida antarctica (CalA) to 0.71 U g BASE −1 , which is almost twofold higher than that previously reported. The specific activity remained in the range of prototype BASE (0.21 U mg protein −1 ), which nevertheless is about 53 times higher than that reported for CalA entrapped in a sol–gel. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library ChemCatChem 3 8 1314 1319 |
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English |
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Abstract Literature reports biocatalytically active static emulsions (BASE) as promising systems for the preparation of biocatalysts designed for synthetic use in organic media. Their excellent catalytic performance is attributed to the numerous micropools of dissolved enzymes independently dispersed in silicone beads. Here, a systematic study of the structure and morphology of BASE and optimization in terms of bead size distribution and overall catalytic performance is presented. The study relies on beads obtained by using a novel preparation method that enables a considerably improved reproducibility of the particle size and catalytic activity in separate batches. A large interfacial area of 0.023 m 2 g −1 material was calculated. The adjustment of bead composition increased the apparent catalytic activity of entrapped lipase A from Candida antarctica (CalA) to 0.71 U g BASE −1 , which is almost twofold higher than that previously reported. The specific activity remained in the range of prototype BASE (0.21 U mg protein −1 ), which nevertheless is about 53 times higher than that reported for CalA entrapped in a sol–gel. |
format |
Article in Journal/Newspaper |
author |
Wu, Changzhu Kraume, Matthias Ansorge‐Schumacher, Marion B. |
spellingShingle |
Wu, Changzhu Kraume, Matthias Ansorge‐Schumacher, Marion B. Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media |
author_facet |
Wu, Changzhu Kraume, Matthias Ansorge‐Schumacher, Marion B. |
author_sort |
Wu, Changzhu |
title |
Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media |
title_short |
Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media |
title_full |
Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media |
title_fullStr |
Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media |
title_full_unstemmed |
Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media |
title_sort |
optimized biocatalytically active static emulsions for organic synthesis in nonaqueous media |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1002/cctc.201100085 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcctc.201100085 http://onlinelibrary.wiley.com/wol1/doi/10.1002/cctc.201100085/fullpdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ChemCatChem volume 3, issue 8, page 1314-1319 ISSN 1867-3880 1867-3899 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cctc.201100085 |
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ChemCatChem |
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1314 |
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1319 |
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1809817011160612864 |