Removing the Active‐Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation
Abstract A mobile region is proposed to be a flap that covers the active site of Candida antarctica lipase A. Removal of the mobile region retains the functional properties of the enzyme. Interestingly interfacial activation, required for the wild‐type enzyme, was not observed for the truncated vari...
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crwiley:10.1002/cbic.201500471 2024-09-15T17:45:11+00:00 Removing the Active‐Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation Wikmark, Ylva Engelmark Cassimjee, Karim Lihammar, Richard Bäckvall, Jan‐E. 2015 http://dx.doi.org/10.1002/cbic.201500471 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbic.201500471 http://onlinelibrary.wiley.com/wol1/doi/10.1002/cbic.201500471/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor ChemBioChem volume 17, issue 2, page 141-145 ISSN 1439-4227 1439-7633 journal-article 2015 crwiley https://doi.org/10.1002/cbic.201500471 2024-08-20T04:14:42Z Abstract A mobile region is proposed to be a flap that covers the active site of Candida antarctica lipase A. Removal of the mobile region retains the functional properties of the enzyme. Interestingly interfacial activation, required for the wild‐type enzyme, was not observed for the truncated variant, although stability, activity, and stereoselectivity were very similar for the wild‐type and variant enzymes. The variant followed classical Michaelis–Menten kinetics, unlike the wild type. Both gave the same relative specificity in the transacylation of a primary and a secondary alcohol in organic solvent. Furthermore, both showed the same enantioselectivity in transacylation of alcohols and the hydrolysis of alcohol esters, as well as in the hydrolysis of esters chiral at the acid part. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library ChemBioChem 17 2 141 145 |
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Wiley Online Library |
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English |
description |
Abstract A mobile region is proposed to be a flap that covers the active site of Candida antarctica lipase A. Removal of the mobile region retains the functional properties of the enzyme. Interestingly interfacial activation, required for the wild‐type enzyme, was not observed for the truncated variant, although stability, activity, and stereoselectivity were very similar for the wild‐type and variant enzymes. The variant followed classical Michaelis–Menten kinetics, unlike the wild type. Both gave the same relative specificity in the transacylation of a primary and a secondary alcohol in organic solvent. Furthermore, both showed the same enantioselectivity in transacylation of alcohols and the hydrolysis of alcohol esters, as well as in the hydrolysis of esters chiral at the acid part. |
format |
Article in Journal/Newspaper |
author |
Wikmark, Ylva Engelmark Cassimjee, Karim Lihammar, Richard Bäckvall, Jan‐E. |
spellingShingle |
Wikmark, Ylva Engelmark Cassimjee, Karim Lihammar, Richard Bäckvall, Jan‐E. Removing the Active‐Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation |
author_facet |
Wikmark, Ylva Engelmark Cassimjee, Karim Lihammar, Richard Bäckvall, Jan‐E. |
author_sort |
Wikmark, Ylva |
title |
Removing the Active‐Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation |
title_short |
Removing the Active‐Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation |
title_full |
Removing the Active‐Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation |
title_fullStr |
Removing the Active‐Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation |
title_full_unstemmed |
Removing the Active‐Site Flap in Lipase A from Candida antarctica Produces a Functional Enzyme without Interfacial Activation |
title_sort |
removing the active‐site flap in lipase a from candida antarctica produces a functional enzyme without interfacial activation |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1002/cbic.201500471 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbic.201500471 http://onlinelibrary.wiley.com/wol1/doi/10.1002/cbic.201500471/fullpdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ChemBioChem volume 17, issue 2, page 141-145 ISSN 1439-4227 1439-7633 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cbic.201500471 |
container_title |
ChemBioChem |
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17 |
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2 |
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141 |
op_container_end_page |
145 |
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1810492922249871360 |