Substrate Conformations Set the Rate of Enzymatic Acrylation by Lipases

• Published in: ChemBioChem
• Main Authors: Syrén, Per‐Olof, Hult, Karl
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2010
• Subjects: Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.1002/cbic.200900758; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbic.200900758; http://onlinelibrary.wiley.com/wol1/doi/10.1002/cbic.200900758/fullpdf

Abstract Acrylates represent a class of α,β‐unsaturated compounds of high industrial importance. We investigated the influence of substrate conformations on the experimentally determined reaction rates of the enzyme‐catalysed transacylation of methyl acrylate and derivatives by ab initio DFT B3LYP calculations and molecular dynamics simulations. The results supported a least‐motion mechanism upon the sp 2 to sp 3 substrate transition to reach the transition state in the enzyme active site. This was in accordance with our hypothesis that acrylates form productive transition states from their low‐energy s‐ sis /s‐ trans conformations. Apparent k cat values were measured for Candida antarctica lipase B (CALB), Humicola insolens cutinase and Rhizomucor miehei lipase and were compared to results from computer simulations. More potent enzymes for acryltransfer, such as the CALB mutant V190A and acrylates with higher turnover numbers, showed elevated populations of productive transition states.