Cross‐reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro
Abstract Cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogen...
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crwiley:10.1002/cbf.942 2024-06-02T08:12:51+00:00 Cross‐reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro Lyons‐Alcantara, Maria Lambkin, Helen A. Nordmo, Rolf Lyng, Fiona Mothersill, Carmel 2002 http://dx.doi.org/10.1002/cbf.942 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbf.942 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbf.942 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Cell Biochemistry and Function volume 20, issue 3, page 247-256 ISSN 0263-6484 1099-0844 journal-article 2002 crwiley https://doi.org/10.1002/cbf.942 2024-05-03T10:46:58Z Abstract Cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and proliferation antigens, developed against mammalian antigens, were tested in paraffin sections of the crustacean Pandalus borealis tissues. Several tissues were analysed: hepatopancreas, gills, ovaries, epithelium under the cuticle and abdominal muscle. Specific antibodies to crustacean proteins are not commercially available. The immunocytochemical results show that antibodies to human epitopes cross‐react with antigens in the crustacean Pandalus borealis indicating that some cellular proteins are highly conserved in evolution. Cytokeratin, proliferating cell nuclear antigen, ras and p‐glycoprotein were detected by immunocytochemistry in Pandalus borealis . No immunoreactivity for Ki‐67 and metallothionein was observed. This system can help in validation and characterization of invertebrate cultures. Copyright © 2002 John Wiley & Sons, Ltd. Article in Journal/Newspaper Pandalus borealis Wiley Online Library Cell Biochemistry and Function 20 3 247 256 |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract Cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and proliferation antigens, developed against mammalian antigens, were tested in paraffin sections of the crustacean Pandalus borealis tissues. Several tissues were analysed: hepatopancreas, gills, ovaries, epithelium under the cuticle and abdominal muscle. Specific antibodies to crustacean proteins are not commercially available. The immunocytochemical results show that antibodies to human epitopes cross‐react with antigens in the crustacean Pandalus borealis indicating that some cellular proteins are highly conserved in evolution. Cytokeratin, proliferating cell nuclear antigen, ras and p‐glycoprotein were detected by immunocytochemistry in Pandalus borealis . No immunoreactivity for Ki‐67 and metallothionein was observed. This system can help in validation and characterization of invertebrate cultures. Copyright © 2002 John Wiley & Sons, Ltd. |
format |
Article in Journal/Newspaper |
author |
Lyons‐Alcantara, Maria Lambkin, Helen A. Nordmo, Rolf Lyng, Fiona Mothersill, Carmel |
spellingShingle |
Lyons‐Alcantara, Maria Lambkin, Helen A. Nordmo, Rolf Lyng, Fiona Mothersill, Carmel Cross‐reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro |
author_facet |
Lyons‐Alcantara, Maria Lambkin, Helen A. Nordmo, Rolf Lyng, Fiona Mothersill, Carmel |
author_sort |
Lyons‐Alcantara, Maria |
title |
Cross‐reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro |
title_short |
Cross‐reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro |
title_full |
Cross‐reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro |
title_fullStr |
Cross‐reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro |
title_full_unstemmed |
Cross‐reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro |
title_sort |
cross‐reactivity of some antibodies to human epitopes with shrimp pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1002/cbf.942 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbf.942 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbf.942 |
genre |
Pandalus borealis |
genre_facet |
Pandalus borealis |
op_source |
Cell Biochemistry and Function volume 20, issue 3, page 247-256 ISSN 0263-6484 1099-0844 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/cbf.942 |
container_title |
Cell Biochemistry and Function |
container_volume |
20 |
container_issue |
3 |
container_start_page |
247 |
op_container_end_page |
256 |
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1800759408189243392 |