Efficient enzymatic synthesis of L‐ascorbyl palmitate using Candida antarctica lipase B‐embedded metal‐organic framework

• Published in: Biotechnology Progress
• Main Authors: Zhang, Xiao‐Jian, Qi, Feng‐Yu, Qi, Jia‐Mei, Yang, Fei, Shen, Jiang‐Wei, Cai, Xue, Liu, Zhi‐Qiang, Zheng, Yu‐Guo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2021
• Subjects: Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.1002/btpr.3218; https://onlinelibrary.wiley.com/doi/pdf/10.1002/btpr.3218; https://onlinelibrary.wiley.com/doi/full-xml/10.1002/btpr.3218

Abstract The Candida antarctica lipase B (CALB) was embedded in the metal‐organic framework, zeolitic imidazolate framework‐8 (ZIF‐8), and applied in the enzymatic synthesis of L ‐ascorbic acid palmitate (ASP) for the first time. The obtained CALB@ZIF‐8 achieved the enzyme loading of 80 mg g −1 with 11.3 U g −1 (dry weight) unit activity, 59.8% activity recovery, and 92.7% immobilization yield. Under the optimal condition, ASP was synthesized with over 75.9% conversion of L ‐ascorbic acid in a 10‐batch reaction. Continuous synthesis of ASP was subsequently performed in a packed bed bioreactor with an outstanding average space–time yield of 58.1 g L −1 h −1 , which was higher than ever reported continuous ASP biosynthesis reactions.