Efficient enzymatic synthesis of L‐ascorbyl palmitate using Candida antarctica lipase B‐embedded metal‐organic framework
Abstract The Candida antarctica lipase B (CALB) was embedded in the metal‐organic framework, zeolitic imidazolate framework‐8 (ZIF‐8), and applied in the enzymatic synthesis of L ‐ascorbic acid palmitate (ASP) for the first time. The obtained CALB@ZIF‐8 achieved the enzyme loading of 80 mg g −1 with...
Published in: | Biotechnology Progress |
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Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2021
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Subjects: | |
Online Access: | http://dx.doi.org/10.1002/btpr.3218 https://onlinelibrary.wiley.com/doi/pdf/10.1002/btpr.3218 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/btpr.3218 |
Summary: | Abstract The Candida antarctica lipase B (CALB) was embedded in the metal‐organic framework, zeolitic imidazolate framework‐8 (ZIF‐8), and applied in the enzymatic synthesis of L ‐ascorbic acid palmitate (ASP) for the first time. The obtained CALB@ZIF‐8 achieved the enzyme loading of 80 mg g −1 with 11.3 U g −1 (dry weight) unit activity, 59.8% activity recovery, and 92.7% immobilization yield. Under the optimal condition, ASP was synthesized with over 75.9% conversion of L ‐ascorbic acid in a 10‐batch reaction. Continuous synthesis of ASP was subsequently performed in a packed bed bioreactor with an outstanding average space–time yield of 58.1 g L −1 h −1 , which was higher than ever reported continuous ASP biosynthesis reactions. |
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