Improved triglyceride transesterification by circular permuted Candida antarctica lipase B
Abstract Lipases represent a versatile class of biocatalysts with numerous potential applications in industry including the production of biodiesel via enzyme‐catalyzed transesterification. In this article, we have investigated the performance of cp283, a variant of Candida antarctica lipase B (CALB...
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crwiley:10.1002/bit.22471 2024-09-15T17:48:12+00:00 Improved triglyceride transesterification by circular permuted Candida antarctica lipase B Yu, Ying Lutz, Stefan 2009 http://dx.doi.org/10.1002/bit.22471 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.22471 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.22471 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology and Bioengineering volume 105, issue 1, page 44-50 ISSN 0006-3592 1097-0290 journal-article 2009 crwiley https://doi.org/10.1002/bit.22471 2024-07-25T04:18:52Z Abstract Lipases represent a versatile class of biocatalysts with numerous potential applications in industry including the production of biodiesel via enzyme‐catalyzed transesterification. In this article, we have investigated the performance of cp283, a variant of Candida antarctica lipase B (CALB) engineered by circular permutation, with a series of esters, as well as pure and complex triglycerides. In comparison with wild‐type CALB, the permutated enzyme showed consistently higher catalytic activity (2.6‐ to 9‐fold) for trans and interesterification of the different substrates with 1‐butanol and ethyl acetate as acyl acceptors. Differences in the observed rates for wild‐type CALB and cp283 are believe to be related to changes in the rate‐determining step of the catalytic cycle as a result of circular permutation. Biotechnol. Bioeng. 2010;105: 44–50. © 2009 Wiley Periodicals, Inc. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Biotechnology and Bioengineering 105 1 44 50 |
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Wiley Online Library |
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language |
English |
description |
Abstract Lipases represent a versatile class of biocatalysts with numerous potential applications in industry including the production of biodiesel via enzyme‐catalyzed transesterification. In this article, we have investigated the performance of cp283, a variant of Candida antarctica lipase B (CALB) engineered by circular permutation, with a series of esters, as well as pure and complex triglycerides. In comparison with wild‐type CALB, the permutated enzyme showed consistently higher catalytic activity (2.6‐ to 9‐fold) for trans and interesterification of the different substrates with 1‐butanol and ethyl acetate as acyl acceptors. Differences in the observed rates for wild‐type CALB and cp283 are believe to be related to changes in the rate‐determining step of the catalytic cycle as a result of circular permutation. Biotechnol. Bioeng. 2010;105: 44–50. © 2009 Wiley Periodicals, Inc. |
format |
Article in Journal/Newspaper |
author |
Yu, Ying Lutz, Stefan |
spellingShingle |
Yu, Ying Lutz, Stefan Improved triglyceride transesterification by circular permuted Candida antarctica lipase B |
author_facet |
Yu, Ying Lutz, Stefan |
author_sort |
Yu, Ying |
title |
Improved triglyceride transesterification by circular permuted Candida antarctica lipase B |
title_short |
Improved triglyceride transesterification by circular permuted Candida antarctica lipase B |
title_full |
Improved triglyceride transesterification by circular permuted Candida antarctica lipase B |
title_fullStr |
Improved triglyceride transesterification by circular permuted Candida antarctica lipase B |
title_full_unstemmed |
Improved triglyceride transesterification by circular permuted Candida antarctica lipase B |
title_sort |
improved triglyceride transesterification by circular permuted candida antarctica lipase b |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1002/bit.22471 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.22471 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.22471 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Bioengineering volume 105, issue 1, page 44-50 ISSN 0006-3592 1097-0290 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bit.22471 |
container_title |
Biotechnology and Bioengineering |
container_volume |
105 |
container_issue |
1 |
container_start_page |
44 |
op_container_end_page |
50 |
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1810289348589912064 |