Options for biochemical production of 4‐hydroxybutyrate and its lactone as a substitute for petrochemical production
Abstract Options are discussed for biochemical production of 4‐hydroxybutyrate (4‐HB) and its lactone, gamma‐butyrolactone (GBL), from renewable sources. In the first part of the study, the thermodynamic feasibility of four potential metabolic pathways from glucose to 4‐HB are analyzed. The calculat...
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crwiley:10.1002/bit.21709 2024-09-09T19:10:10+00:00 Options for biochemical production of 4‐hydroxybutyrate and its lactone as a substitute for petrochemical production Efe, C. Straathof, Adrie J.J. van der Wielen, Luuk A.M. 2007 http://dx.doi.org/10.1002/bit.21709 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.21709 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.21709 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology and Bioengineering volume 99, issue 6, page 1392-1406 ISSN 0006-3592 1097-0290 journal-article 2007 crwiley https://doi.org/10.1002/bit.21709 2024-08-27T04:31:54Z Abstract Options are discussed for biochemical production of 4‐hydroxybutyrate (4‐HB) and its lactone, gamma‐butyrolactone (GBL), from renewable sources. In the first part of the study, the thermodynamic feasibility of four potential metabolic pathways from glucose to 4‐HB are analyzed. The calculations reveal that when the pathways are NAD + dependent the intermediate succinate semialdehyde (SSA) accumulates leading to low 4‐HB yields at equilibrium. For NADP + dependent pathways the calculated yield of 4‐HB improves, up to almost 100%. In the second part of this study, continuous removal of 4‐HB from the solution is considered to shift SSA conversion into 4‐HB so that SSA accumulation is minimized. One option is the enzymatic production of GBL from 4‐HB. Candida antarctica Lipase B shows good lactonization rates at pH 4, but unfortunately this conversion cannot be performed in‐vivo during 4‐HB production because of the neutral intracellular pH. Biotechnol. Bioeng. 2008;99: 1392–1406. © 2007 Wiley Periodicals, Inc. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Biotechnology and Bioengineering 99 6 1392 1406 |
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English |
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Abstract Options are discussed for biochemical production of 4‐hydroxybutyrate (4‐HB) and its lactone, gamma‐butyrolactone (GBL), from renewable sources. In the first part of the study, the thermodynamic feasibility of four potential metabolic pathways from glucose to 4‐HB are analyzed. The calculations reveal that when the pathways are NAD + dependent the intermediate succinate semialdehyde (SSA) accumulates leading to low 4‐HB yields at equilibrium. For NADP + dependent pathways the calculated yield of 4‐HB improves, up to almost 100%. In the second part of this study, continuous removal of 4‐HB from the solution is considered to shift SSA conversion into 4‐HB so that SSA accumulation is minimized. One option is the enzymatic production of GBL from 4‐HB. Candida antarctica Lipase B shows good lactonization rates at pH 4, but unfortunately this conversion cannot be performed in‐vivo during 4‐HB production because of the neutral intracellular pH. Biotechnol. Bioeng. 2008;99: 1392–1406. © 2007 Wiley Periodicals, Inc. |
format |
Article in Journal/Newspaper |
author |
Efe, C. Straathof, Adrie J.J. van der Wielen, Luuk A.M. |
spellingShingle |
Efe, C. Straathof, Adrie J.J. van der Wielen, Luuk A.M. Options for biochemical production of 4‐hydroxybutyrate and its lactone as a substitute for petrochemical production |
author_facet |
Efe, C. Straathof, Adrie J.J. van der Wielen, Luuk A.M. |
author_sort |
Efe, C. |
title |
Options for biochemical production of 4‐hydroxybutyrate and its lactone as a substitute for petrochemical production |
title_short |
Options for biochemical production of 4‐hydroxybutyrate and its lactone as a substitute for petrochemical production |
title_full |
Options for biochemical production of 4‐hydroxybutyrate and its lactone as a substitute for petrochemical production |
title_fullStr |
Options for biochemical production of 4‐hydroxybutyrate and its lactone as a substitute for petrochemical production |
title_full_unstemmed |
Options for biochemical production of 4‐hydroxybutyrate and its lactone as a substitute for petrochemical production |
title_sort |
options for biochemical production of 4‐hydroxybutyrate and its lactone as a substitute for petrochemical production |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1002/bit.21709 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.21709 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.21709 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Bioengineering volume 99, issue 6, page 1392-1406 ISSN 0006-3592 1097-0290 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bit.21709 |
container_title |
Biotechnology and Bioengineering |
container_volume |
99 |
container_issue |
6 |
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1392 |
op_container_end_page |
1406 |
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1809824754944704512 |