Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale
Abstract Lipase type B from Candida antarctica was used to catalyze the esterification of propionic acid and 1‐butanol in a water/ n ‐decane two‐phase system on micro and on bench scale. The reaction was described by a Ping Pong Bi Bi mechanism with alcohol inhibition. The kinetic parameters on micr...
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crwiley:10.1002/bit.21650 2024-06-23T07:47:27+00:00 Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale Swarts, Jan W. Vossenberg, Petra Meerman, Marieke H. Janssen, Anja E.M. Boom, Remko M. 2008 http://dx.doi.org/10.1002/bit.21650 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.21650 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.21650 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology and Bioengineering volume 99, issue 4, page 855-861 ISSN 0006-3592 1097-0290 journal-article 2008 crwiley https://doi.org/10.1002/bit.21650 2024-05-31T08:10:20Z Abstract Lipase type B from Candida antarctica was used to catalyze the esterification of propionic acid and 1‐butanol in a water/ n ‐decane two‐phase system on micro and on bench scale. The reaction was described by a Ping Pong Bi Bi mechanism with alcohol inhibition. The kinetic parameters on micro and bench scale were compared; no significant differences were found. Furthermore, effects of temperature on activation and inactivation of the enzyme were found to be similar on micro and bench scale. Therefore, parameters found on either scale can be used for the other scale. Enzyme kinetic parameters can be determined on a micro scale, with very low consumption of reagents and catalyst, and then be applied to bench scale. This can reduce the cost of optimizing enzyme processes by downscaling. Biotechnol. Bioeng. 2008;99: 855–861. © 2007 Wiley Periodicals, Inc. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Biotechnology and Bioengineering 99 4 855 861 |
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Wiley Online Library |
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English |
description |
Abstract Lipase type B from Candida antarctica was used to catalyze the esterification of propionic acid and 1‐butanol in a water/ n ‐decane two‐phase system on micro and on bench scale. The reaction was described by a Ping Pong Bi Bi mechanism with alcohol inhibition. The kinetic parameters on micro and bench scale were compared; no significant differences were found. Furthermore, effects of temperature on activation and inactivation of the enzyme were found to be similar on micro and bench scale. Therefore, parameters found on either scale can be used for the other scale. Enzyme kinetic parameters can be determined on a micro scale, with very low consumption of reagents and catalyst, and then be applied to bench scale. This can reduce the cost of optimizing enzyme processes by downscaling. Biotechnol. Bioeng. 2008;99: 855–861. © 2007 Wiley Periodicals, Inc. |
format |
Article in Journal/Newspaper |
author |
Swarts, Jan W. Vossenberg, Petra Meerman, Marieke H. Janssen, Anja E.M. Boom, Remko M. |
spellingShingle |
Swarts, Jan W. Vossenberg, Petra Meerman, Marieke H. Janssen, Anja E.M. Boom, Remko M. Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale |
author_facet |
Swarts, Jan W. Vossenberg, Petra Meerman, Marieke H. Janssen, Anja E.M. Boom, Remko M. |
author_sort |
Swarts, Jan W. |
title |
Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale |
title_short |
Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale |
title_full |
Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale |
title_fullStr |
Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale |
title_full_unstemmed |
Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale |
title_sort |
comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1002/bit.21650 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.21650 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.21650 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Bioengineering volume 99, issue 4, page 855-861 ISSN 0006-3592 1097-0290 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bit.21650 |
container_title |
Biotechnology and Bioengineering |
container_volume |
99 |
container_issue |
4 |
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855 |
op_container_end_page |
861 |
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1802651557430296576 |