Economically pertinent continuous amide formation by direct lipase‐catalyzed amidation with ammonia
Abstract An economically pertinent process for the lipase‐catalyzed synthesis of amides was developed. A continuous plug flow reactor was used. The model reaction was the production of oleamide, a lubricant and anti‐slip agent, via direct Candida antarctica lipase B‐catalyzed amidation of oleic acid...
Published in: | Biotechnology and Bioengineering |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2003
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/bit.10613 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.10613 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10613 |
id |
crwiley:10.1002/bit.10613 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/bit.10613 2024-06-23T07:47:27+00:00 Economically pertinent continuous amide formation by direct lipase‐catalyzed amidation with ammonia Slotema, Wouter F. Sandoval, Georgina Guieysse, David Straathof, Adrie J.J. Marty, Alain 2003 http://dx.doi.org/10.1002/bit.10613 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.10613 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10613 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology and Bioengineering volume 82, issue 6, page 664-669 ISSN 0006-3592 1097-0290 journal-article 2003 crwiley https://doi.org/10.1002/bit.10613 2024-06-13T04:24:08Z Abstract An economically pertinent process for the lipase‐catalyzed synthesis of amides was developed. A continuous plug flow reactor was used. The model reaction was the production of oleamide, a lubricant and anti‐slip agent, via direct Candida antarctica lipase B‐catalyzed amidation of oleic acid with ammonia. Of all solvents tested, 2‐methyl‐2‐butanol was found to respond optimally to the demands formulated in our specifications. A continuous conversion of oleic acid into oleamide of 85% was obtained. A productivity of 4.5 tons oleamide per kg of enzyme per year was calculated, indicating a contribution of enzyme to the product price of only 4%. The volumetric productivity, 100 g · L −1 · h −1 , is 4 to 100 times higher than in literature procedures. A simple crystallization procedure leads to 99% purity. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 664–669, 2003. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Biotechnology and Bioengineering 82 6 664 669 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract An economically pertinent process for the lipase‐catalyzed synthesis of amides was developed. A continuous plug flow reactor was used. The model reaction was the production of oleamide, a lubricant and anti‐slip agent, via direct Candida antarctica lipase B‐catalyzed amidation of oleic acid with ammonia. Of all solvents tested, 2‐methyl‐2‐butanol was found to respond optimally to the demands formulated in our specifications. A continuous conversion of oleic acid into oleamide of 85% was obtained. A productivity of 4.5 tons oleamide per kg of enzyme per year was calculated, indicating a contribution of enzyme to the product price of only 4%. The volumetric productivity, 100 g · L −1 · h −1 , is 4 to 100 times higher than in literature procedures. A simple crystallization procedure leads to 99% purity. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 664–669, 2003. |
format |
Article in Journal/Newspaper |
author |
Slotema, Wouter F. Sandoval, Georgina Guieysse, David Straathof, Adrie J.J. Marty, Alain |
spellingShingle |
Slotema, Wouter F. Sandoval, Georgina Guieysse, David Straathof, Adrie J.J. Marty, Alain Economically pertinent continuous amide formation by direct lipase‐catalyzed amidation with ammonia |
author_facet |
Slotema, Wouter F. Sandoval, Georgina Guieysse, David Straathof, Adrie J.J. Marty, Alain |
author_sort |
Slotema, Wouter F. |
title |
Economically pertinent continuous amide formation by direct lipase‐catalyzed amidation with ammonia |
title_short |
Economically pertinent continuous amide formation by direct lipase‐catalyzed amidation with ammonia |
title_full |
Economically pertinent continuous amide formation by direct lipase‐catalyzed amidation with ammonia |
title_fullStr |
Economically pertinent continuous amide formation by direct lipase‐catalyzed amidation with ammonia |
title_full_unstemmed |
Economically pertinent continuous amide formation by direct lipase‐catalyzed amidation with ammonia |
title_sort |
economically pertinent continuous amide formation by direct lipase‐catalyzed amidation with ammonia |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1002/bit.10613 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.10613 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10613 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Bioengineering volume 82, issue 6, page 664-669 ISSN 0006-3592 1097-0290 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bit.10613 |
container_title |
Biotechnology and Bioengineering |
container_volume |
82 |
container_issue |
6 |
container_start_page |
664 |
op_container_end_page |
669 |
_version_ |
1802651557825609728 |